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Effect of human endogenous retrovirus-K env gene knockout on proliferation of ovarian cancer cells
Ko Eun-Ji,Kim Eun Taeg,Kim Heungyeol,Lee Chul Min,Koh Suk Bong,Eo Wan Kyu,Kim Hongbae,Oh Young Lim,Ock Mee Sun,Kim Ki Hyung,Cha Hee-Jae 한국유전학회 2022 Genes & Genomics Vol.44 No.9
Background: Among various human endogenous retroviruses (HERVs), the HERV-K (HML-2) group has been reported to be highly related to cancer. In pancreatic cancer cells, shRNA-mediated downregulation of HERV-K env RNA decreases cell proliferation and tumor growth through the RAS-ERK-RSK pathway; in colorectal cancer, CRISPR-Cas9 knockout (KO) of the HERV-K env gene affects tumorigenic characteristics through the nupr-1 gene. Objective: The effect of HERV-K env KO has not been studied in ovarian cancer cell lines. In this study, we analyzed the tumorigenic characteristics of ovarian cancer cell lines, including cell proliferation, migration, and invasion, and the expression patterns of related proteins after CRISPR-Cas9 KO of the HERV-K env gene. Methods: The HERV-K env gene KO was achieved using the CRISPR-Cas9 system in ovarian cancer cell lines SKOV3 and OVCAR3. Tumorigenic characteristics including cell proliferation, migration, and invasion were analyzed, and related protein expression was investigated by western blot analysis. Results: The expression of the HERV-K env gene in KO cells was significantly reduced at RNA and protein levels, and tumorigenic characteristics including cell proliferation, migration, and invasion were significantly reduced. In HERV-K env KO SKOV3 cells, the expression of the RB protein was significantly up-regulated and the cyclin B1 protein level was significantly reduced. In contrast, in HERV-K env KO OVCAR3 cells, the level of phospho-RB protein was significantly reduced, but other protein levels were not changed. Conclusion: The results of this study showed that HERV-K env gene KO affects cell proliferation, invasion, and migration of ovarian cells through RB and Cyclin B1 proteins, but the specific regulation pattern can differ by cell line.
Kim Do-Ye,Kim Heungyeol,Ko Eun-Ji,Koh Suk Bong,Kim Hongbae,Lee Ji Young,Lee Chul Min,Eo Wan Kyu,Kim Ki Hyung,Cha Hee-Jae 한국유전학회 2024 Genes & Genomics Vol.46 No.4
Background Human endogenous retrovirus (HERV)-K is a type of retrovirus that is present in the human genome, and its expression is usually silenced in healthy tissues. The precise mechanism by which HERV-K env influences cancer stemness is not fully understood, but it has been suggested that HERV-K env may activate various signaling pathways that promote stemness traits in cancer cells. Objective To establish the connection between HERV-K env expression and cancer stemness in ovarian cancer cells, we carried out correlation analyses between HERV-K env and the cancer stem cell (CSC) marker known as the cluster of differentiation 133 (CD133) gene in SKOV3 ovarian cancer cells. Method To perform correlation analysis between HERV-K env and CSCs, ovarian cancer cells were cultured in a medium designed for cancer stem cell induction. The expression of HERV-K env and CD133 genes was verified using quantitative real-time polymerase chain reaction (RT-qPCR) and Western blot analyses. Additionally, the expression of stemness-related markers, such as OCT-4 and Nanog, was also confirmed using RT-qPCR. Results In the stem cell induction medium, the number of tumorsphere-type SKOV3 cells increased, and the expression of CD133 and HERV-K env genes was up-regulated. Additionally, other stemness-related markers like OCT-4 and Nanog also exhibited increased expression when cultured in the cancer stem cell induction medium. However, when HERV-K env knockout (KO) SKOV3 cells were cultured in the same cancer stem cell induction medium, there was a significant decrease in the number of tumorsphere-type cells compared to mock SKOV3 cells subjected to the same conditions. Furthermore, the expression of CD133, Nanog, and OCT-4 did not show a significant increase in HERV-K env KO SKOV3 cells compared to mock SKOV3 cells cultured in the same cancer stem cell induction medium. Conclusion These findings indicate that the expression of HERV-K env increased in SKOV3 cells when cultured in cancer stem cell induction media, and cancer stem cell induction was inhibited by KO of HERV-K env in SKOV3 cells. These results suggest a strong association between HERV-K env and stemness in SKOV3 ovarian cancer cells. Background Human endogenous retrovirus (HERV)-K is a type of retrovirus that is present in the human genome, and its expression is usually silenced in healthy tissues. The precise mechanism by which HERV-K env influences cancer stemness is not fully understood, but it has been suggested that HERV-K env may activate various signaling pathways that promote stemness traits in cancer cells. Objective To establish the connection between HERV-K env expression and cancer stemness in ovarian cancer cells, we carried out correlation analyses between HERV-K env and the cancer stem cell (CSC) marker known as the cluster of differentiation 133 (CD133) gene in SKOV3 ovarian cancer cells. Method To perform correlation analysis between HERV-K env and CSCs, ovarian cancer cells were cultured in a medium designed for cancer stem cell induction. The expression of HERV-K env and CD133 genes was verified using quantitative real-time polymerase chain reaction (RT-qPCR) and Western blot analyses. Additionally, the expression of stemness-related markers, such as OCT-4 and Nanog, was also confirmed using RT-qPCR. Results In the stem cell induction medium, the number of tumorsphere-type SKOV3 cells increased, and the expression of CD133 and HERV-K env genes was up-regulated. Additionally, other stemness-related markers like OCT-4 and Nanog also exhibited increased expression when cultured in the cancer stem cell induction medium. However, when HERV-K env knockout (KO) SKOV3 cells were cultured in the same cancer stem cell induction medium, there was a significant decrease in the number of tumorsphere-type cells compared to mock SKOV3 cells subjected to the same conditions. Furthermore, the expression of CD133, Nanog, and OCT-4 did not show a significant increase in HERV-K env KO SKOV3 cells compared to mock SKOV3 cells cultured in the same cancer stem cell induction medium. Conclusion These findings indicate that the expression of HERV-K env increased in SKOV3 cells when cultured in cancer stem cell induction media, and cancer stem cell induction was inhibited by KO of HERV-K env in SKOV3 cells. These results suggest a strong association between HERV-K env and stemness in SKOV3 ovarian cancer cells.
Vi Capsular Polysaccharide(Vi CPS) 장티푸스 예방접종후 항체가의 변동 및 안전성
고대균,박지원,신완식,강문원,강진한 대한감염학회 1991 감염 Vol.23 No.3
Typhoid fever is still endemic in developing countries, and in many it poses a major public health problem. Although the antibiotic has improved prognosis and reduced mortality, persistence of mortality due to delayed introduction of treatment and appearance of resistant strains. It is therefore important to prevent this disease, and vaccination becomes the preferred means of prevention. Until now, the available vaccines, prepared from inactivated micro organisms, required several injections and caused serious adverse reactions. An oral vaccine composed of a live, attenuated strain is also available, but because repeated doses are required, its use in the fileld is of limited value. To evaluate the immunogenicity and safety of the Vi capsular polysaccharide (CPS) of Salmonella typhi, a single dose of 25㎍ of the Vi CPS was injected intramuscularly to 85 healthy children aged 5-15 years and 85 healthy young volunteers. None of these volunteers had previously suffered from typhoid fever and been vaccinated against typhoid fever. Adverse effects were assessed on day 0, 1, 2 and 3 according to clinical chart which was fulfilled by the subjects themselves. Anti-Vi antibody titers were measured by passive hemagglutination test before and 1, 3, and 12 months after vaccination. The side effects of Vi CPS were limited to minor and transient local reactions. Slight pain at the site of injection (47.1%), with erythema (4.7%) or induration (16.5%). Shivering was observed in only one case. The rate of seroconversion was 93.5% at 1 month (98.8% in children, 88.2% in adults). In eight of eleven non-seroconverters, the titers were increased four folds or more at 3 months. The antibody titers remained plateau for at least 12 months. To see whether this acquired level of Vi antibody titers protects vaccinees from typhoid fever, further clinical and epidemiological studies need to be performed.
열정산 방법을 통한 사업장폐기물 소각시설의 출열분포 특성
손지환 ( Ji Hwan Son ),유하녕 ( Ha Nyoung Yoo ),강준구 ( Jun Gu Kang ),고영재 ( Young Jae Ko ),권영현 ( Young Hyun Kwon ),전태완 ( Tae Wan Jeon ),신선경 ( Sun Kyoung Shin ) 한국폐기물자원순환학회(구 한국폐기물학회) 2017 한국폐기물자원순환학회 추계학술발표논문집 Vol.2017 No.-
자원의 효율적 이용 및 폐기물의 자원화를 통해 천연자원의 소비를 감축하고자 환경부에서는 2018년 1월 1일부터 자원순환기본법이 시행된다. 기본원칙으로 폐기물의 발생을 최대한 억제하고 발생된 폐기물에 대해서는 재사용하고 재사용이 곤란한 경우 재생이용하고 둘 다 곤란한 경우에는 최대한 에너지를 회수·이용하여 열원(온수, 증기 등) 또는 전기 등의 에너지로 활용하고자한다. 이에 따라 소각시설에서 에너지 생산량에 기여하는 출열 분포의 중요성이 높아지고 있다. 따라서 본 연구에서는 사업장폐기물 소각시설을 대상으로 각각의 출열인자별 양을 산정하고 출열분포 특성에 대하여 고찰하고자 한다. 본 연구는 사업장폐기물 소각시설 7개 시설(10개 호기)를 대상으로 진행하였다. 대상시설의 출열항목은 폐열 보일러의 설치형태(일체형, 분리형)에 따라 결정하였다. 소각로와 보일러가 붙은 경우를 일체형이라 하고 증기 흡수열, 배출가스 보유열, 보일러 방열손실, 소각로 방열손실, 바닥재 배출열, 미연탄소분 열손실, 블로우다운 배출열의 총 7가지 출열항목을 산정하였으며, 소각로와 보일러가 분리되어 있는 경우를 분리형이라 하여 배출가스 보유열, 소각로 방열손실, 바닥재 배출열, 미연탄소분 열손실의 총 4가지 출열항목을 산정하였다. 이를 출열분포를 산정하기 위해 계측기를 이용하여 관련한 데이터를 일별로 수집하였으며, 계측이 되지 않는 항목에 대하여는 직접 측정하여 산정하였다. 출열분포 특성을 살펴본 결과 일체형 보일러를 설치한 소각시설의 경우 증기 흡수열이 출열분포가 큰 것으로 나타났으며, 분리형 보일러를 설치한 소각시설은 배출가스 보유열이 가장 많이 차지하는 것으로 나타났다.