암 환자의 혈장 Transforming Growth Factor-β1 농도

박병규,하우송,이시은,이수진,박순태,박찬후,전지현,장정순 THE KOREAN SOCIETY FOR BIOMEDICAL LABORATORY SCINE 1999 Journal of biomedical laboratory sciences Vol.5 No.2

한국인의 대표적인 성인 고형 종양인 위암, 간암, 유방암과 소아 백혈병 및 2종의 소아 고형 종양 환자로부터 혈장 transforming growth factor-ß1 (TGF-ß1) 농도를 sandwich ELISA 분석법을 이용해 측정함으로써 TGF-ß1을 이 질환들에 대한 새로운 종양표지자 (tumor marker)로 사용할 수 있는지 검토하였다. 또한 연령 및 성별에 따른 혈장 TGF-ß1 농도의 정상치를 조사하였다. 신생아에서 70대까지 혈장 TGF-ß1 농도의 차이는 없었고 남녀간의 차이도 없었다. 위암 환자의 혈장TCF-ß1 농도는 16.0±6.8 ng/ml (평균 ±표준편차)로 정상 대조군의 TGF-ß1 농도 (8.3 ±5.0 ng/ml) 보다 유의하게 높았으나 간암, 유방암 환자의 혈장 TGF-ß1 농도는 대조군과 차이가 없었다. 그리고 위암 환자 16명, 간암 환자 8명, 유방암 환자 7명 중 각각 7명 (43.7%), 1명 (12.5%), 1명 (14.3%)에서만 혈장 TGF-ß1 농도가 증가되었다. 5명의 소아 백혈병 환자에서는 관해 (remission) 여부와 상관없이 혈장 TGF-ßl 농도가 모두 정상 범위에 있었으나 2명의 소아 고형암 환자에서는 종양 절제 전에는 혈장TGF-ß1 농도가 높았다가 절제 후 정상으로 떨어졌다. 결론적으로 1)정상인의 혈장 TGF-ßl 농도는 연령 및 성별에 따른 차이가 없다는 것을 알 수 있었고, 2)성인 고형암인 위암, 간암, 유방암에서는 낮은 민감도로 인해 TGF-ß1을 진단을 위한 선별 검사로 이용하기에는 부적절한 것으로 판단되었으며, 3) 정상 대조군보다 혈장 TGF-ß1 농도가 높았던 위암 환자와 종양 절제 전후로 혈장 TGF-ß1 농도가 민감하게 변했던 소아 고형 암 환자에 대해서는 향후 표본 수를 늘려 부가적인 연구를 해 야 할 것으로 사료된다. To evaluate the usefulness of transforming growth factor-ß1 (TGF-ß1)as a new tumor marker, we determined the plasma TGF-ß1 levels using sandwich ELISA assay in cancer patients. Patients with three most common adult cancers in Korea (stomach, liver and breast cancer) and children's cancers (leukemia and two kinds of solid tumor) were enrolled for the study. Furthermore, 39 individuals were subjected to age and sex-stratified plasma TGF-ß1 analysis. No statistical difference was demonstrated with respect to age or sex. The mean plasma TGF-ß1 level (16.0 ng/ml) of stomach cancer patients was significantly higher than that (8.3ng/ml) of controls. However, there was no difference among the mean plasma TGF-ß1 levels of liver, breast cancer patients and controls. Seven of 16 patients (43.7%) with stomach cancer, one of 8 (12.5%) with liver cancer, and one of 7 (14.3%) with breast cancer showed higher TGF-ß1 levels compared to controls. Plasma TGF-ß1 concentrations of five leukemic children remained in the normal range regardless of the remission state. In contrast, initial high TGF-ß1 levels from two children with solid tumors returned to normal range on surgical resection of tumors. From the above results, we could conclude that plasma TGF-ß1 levels of apparently healthy individuals seem to be rather constant irrespective of difference in age or sex, and the plasma TGF-ß1 has the limited value as a screening test for the diagnosis of aforementioned adult cancers because of its low sensitivity. Finally, additional studies need to be pursed for the large number of stomach cancer and pediatric solid tumor patients in order to reach a secure conclusion on the usefulness of plasma TGF-ß1 as a tumor marker in these patients.

디지털 I-PD 제어기에 의한 태양광 발전 시스템의 정출력 제어

박지호,김영빈,신동률,문백영,우정인 東亞大學校 大學院 2000 大學院論文集 Vol.25 No.-

In this paper, constant output control of solar power generation system with instantaneous variable output is proposed. In order to get constant DC and AC voltage source, we construct Buck-Boost chopper equip with the digital I-PD type controller first. Consequently, we are able to get constant DC boltage. Next, we obtain AC source through construction PWM single phase inverter that has the constant frequency output. And we design LC filter to reduce THD(Total Harmonic Distortion). The validity of proposed control strategy is verified from results of simulation.

유산균들의 콜레스테를 저하성, 내산성, 내담즙성, 항생제 내성 비교

박소영,고영태,정후길,양진오,정현서,김영배,지근억 한국미생물생명공학회 ( 구 한국산업미생물학회 ) 1996 한국미생물·생명공학회지 Vol.24 No.3

기능성 요구르트의 제조에 사용되는 유산균은 인체에 유익한 생리활성과 우수한 생존능력을 보유하고 있는 것이 바람직하다. 본 연구에서는 다양한 유산균주의 산과 담즙 및 항생제에 대한 내성을 조사하였으며 in vitro에서 콜레스테롤 저하능을 조사하였다. In vitro에서 콜레스테롤 저하능이 우수한 13균주를 선발하여 이들의 동결건조 분말을 고 콜레스테롤 식이의 실험쥐에 투여하였다. 그 결과 8균주는 비 투여구보다 유의적으로 18.3~27.3%의 콜레스테롤 저하능을 나타냈다(P≤0.05). 이들 중에서 Bifidobacterium infantis AM-220, Lactobacillus AM-245, Streptococcus MA-1의 3균주를 이용하여 요구르트를 제조한 후 동결건조 분말을 이용하여 급여실험을 수행한 결과, 총 콜레스테롤과 LDL 함량이 유의적으로 감소하는 것으로 나타났다. For a probiotic yoghurt it is desirable to utilize lactic acid bacteria with a high survival rate and beneficial function to human beings. We have examined a variety of lactic acid bacteria to assess the acid and bile tolerance and antibiotic resistance. In addition, an in vitro culture experiment was performed to evaluate their ability to reduce cholesterol levels in the growth medium. Thirteen strains were selected from in vitro cholesterol assays and fed to Sprague-Dawley rats with a high-cholesterol diet. Among the 13 strains tested, 8 strains were shown to reduce serum cholesterol levels significantly after 24 days of administration in vivo. Rats were fed lyophilized yoghurt powder fermented with a combination of 3 selected strains: Bifidobacterium infantis AM-220, Lactobacillus AM-245, and Streptococcus MA-1. The levels of total cholesterol and low density lipoprotein were significantly lower (p≤0.05) in rats fed the yoghurt powder compared with control group. These studies suggest that yoghurt fermented with appropriately selected lactic acid bacteria may have a anticholesterolemic effect.

폐 내 병변으로 인한 소아 급성 호흡부전의 원인 질환과 예후

이혜영,박지숙,최희라,서지현,김점수,임재영,최명범,박찬후,우향옥,윤희상 대한소아과학회 2004 小兒科 Vol.47 No.5

요중 N-acetyl-β-D-glucosaminidase 활성치를 이용한 신발제조업 근로자들의 만성 신기능 장애 평가

김돈균,이수일,조병만,이지호,이후락,박종욱 大韓産業醫學會 1993 대한직업환경의학회지 Vol.5 No.1

부산지역 신발제조업 근로자중 유기용제에 폭로되고 있는 150명을 폭로군으로 하고, 일반사무직 근로자 53명을 대조군으로 하여 인적특성, 직업에 대한 만족도, 자각증상, 작업과 관련된 스트레스의 정도를 조사하고, 유기용제 폭로량의 지표가 되는 요중 마뇨산 및 근로자 건강진단 항목인 혈중 요소질소, 혈청 크레아티닌 그리고 조기 신기능 장애의 지표로 알려진 요중 NAG 활성치를 측정하여 얻은 바를 아래와 같이 요약한다. 1. 요중 NAG 활성치는 폭로군에서 4.63U/g creatinine, 대조군에서 2.95U/g creatinine로 폭로군에서 높게 측정되었고(p<0.05), 유기용제 폭로에 의한 신기능 변화에 민감한 결과를 보였다. 2. 설문조사에서 직업에 대한 불만족, 이직을 원하는 경우, 건강에 대한 염려, 직업과 관련된 스트레스 및 유기용제 폭로와 관련된 증상호소가 대조군에 비해 폭로군에서 유의하게 증가되어 있어(p<0.05), 유기용제에 폭로되고 있는 신발제조업 근로자들의 보건관리는 산업보건학적 측면에서 매우 중요한 것으로 나타났다. 3. 폭로군중 근무기간 5년 이상인 대상자에서 요중 NAG 활성치는 5.92U/g creatinine, 근무기간 5년 미만인 경우 4.34U/g creatinine으로, 유기용제 폭로 기간이 길어짐에 따라 요중 NAG활성치는 통계학적으로 유의하게 증가되어 있었다(p<0.05). 4. 요중 NAG활성치는 근무기간을 가중한 톨루엔 총 폭로량과 유의한 정의 상관관계를 보여(p<0.05), 용량-반응관계를 보여 주었다. 이상의 결과에서, 복합유기용제에 만성적으로 폭로되는 근로자들에 공통적으로 발생할 수 있는 조기 신기능 장애를 감시하기 위하여, 요중 NAG활성치를 측정한 자료를 이용하면 유기용제 폭로 근로자들의 보건관리를 위하여 자료를 제공할 수 있을 것으로 생각된다. Personal characteristics, degree of satisfaction for work, subjective symptoms, and stress related to working condition were investigated from 150 workers exposed to organic solvents in shoe factories and 53 official workers in Pusan area, and urinary hippuric acid concentration which represented the amount of exposed organic solvents, BUN and serum creatinine which were renal function tests for worker's health examination and urinary N-acetyl-β-D-glucosaminidase activity were measured. The results obtained were summarized as follows. 1. The mean value of urinary NAG activity of exposed group was higher than that of control group(p<0.01), and subjects whose NAG activities were over refrence range in exposed group were far more than in control group(p<0.05), therefore urinary NAG activity was a sensitive test for the change of renal function due to exposure of organic solvents. 2. Unsatisfaction for occupation, want to change occupation, worry about health, stress related to job and symptoms related to exposure of organic solvents investigated by questionnaire were higher in exposed group than in control group, therefore the health management for workers exposed to organic solvents in shoe factories was important in the viewpoint of occupational medicine. 3. The mean value of urinary NAG activity in the subjects whose working duration were over 5 years was higher than in the subjects whose working duration were over 5 years was higher than in the subjects whose working duration were under 5 years, therefore the longer exposured duration was, the higher urinary NAG activity was. 4. Urinary NAG activity had positive correlation with time weighted amount of total toluene exposure therefore dose-response relationship was suspected. As above results, it will be very useful to apply the data of urinary NAG activity to the health management of workers exposed to orgaic solvents in order to monitoring of early renal dysfunction which was common in the workers chornically exposed to mixed organic solvents.

Differences in Clinical and Laboratory Findings between Group D and Non-Group D Non-Typhoidal Salmonella Gastroenteritis in Children

Park, Heung Keun,Rhie, Kyuyol,Yeom, Jung Sook,Park, Ji Sook,Park, Eun Sil,Seo, Ji-Hyun,Lim, Jae Young,Park, Chan-Hoo,Woo, Hyang-Ok,Youn, Hee-Shang,Kang, Ki Ryeon,Park, Jung Je The Korean Society of Pediatric Gastroenterology 2015 Pediatric gastroenterology, hepatology & nutrition Vol.18 No.2

Purpose: To investigate the differences in clinical features and laboratory findings between group D and non-group D non-typhoidal Salmonella (NTS) gastroenteritis in children. Methods: A retrospective chart review of children diagnosed with NTS confirmed by culture study was performed. The clinical features and laboratory findings of group D and non-group D NTS were compared. Results: From 2003 to 2012, 75 cases were diagnosed as NTS at our center. The number of group D and non-group D patients was 45 and 30, respectively. The mean age was higher in group D than in non-group D patients (5.1 years vs. 3.4 years, p=0.038). Headaches were more frequently observed (p=0.046) and hematochezia was less frequently observed (p=0.017) in group D than in non-group D NTS gastroenteritis patients. A positive Widal test result was observed in 53.3% of group D and 6.7% of non-group D NTS cases (O-titer, p=0.030; H-titer, p=0.039). There were no differences in white blood cell counts, level of C-reactive protein and rate of antimicrobial resistance between group D and non-group D cases. Conclusion: The more severe clinical features such as headache, fever, and higher Widal titers were found to be indicative of group D NTS gastroenteritis. Additionally, group D NTS gastroenteritis was more commonly found in older patients. Therefore, old age, fever, headache, and a positive Widal test are more indicative of group D NTS than non-group D NTS gastroenteritis. Pathophysiological mechanisms may differ across serologic groups.

The clinical significance of intra-opreative endoscopy for tumor localization in totally laparoscopic partial gastrectomy

Shin-Hoo PARK,Hyuk-Joon LEE,Jong-Ho CHOI,Ji-Hyun PARK,Ji-Ho PARK,Tae-Han KIM,Yun-Suhk SUH,Seong-Ho KONG,Do Joong PARK,Han-Kwang YANG 대한외과학회 2019 대한외과학회 학술대회 초록집 Vol.2019 No.10

A novel mutation of ABCC8 gene in a patient with diazoxide-unresponsive congenital hyperinsulinism

Park, Ji Sook,Lee, Hong-Jun,Park, Chan-Hoo The Korean Pediatric Society 2016 Clinical and Experimental Pediatrics (CEP) Vol.59 No.no.sup1

Congenital hyperinsulinism (CHI) is a rare condition that can cause irreversible brain damage during the neonatal period owing to the associated hypoglycemia. Hypoglycemia in CHI occurs secondary to the dysregulation of insulin secretion. CHI has been established as a genetic disorder of islet-cell hyperplasia, associated with a mutation of the ABCC8 or KCNJ11 genes, which encode the sulfonylurea receptor 1 and the inward rectifying potassium channel (Kir6.2) subunit of the ATP-sensitive potassium channel, respectively. We report the case of a female newborn infant who presented with repetitive seizures and episodes of apnea after birth, because of hypoglycemia. Investigations revealed hypoglycemia with hyperinsulinemia, but no ketone bodies, and a low level of free fatty acids. High dose glucose infusion, enteral feeding, and medications could not maintain the patient's serum glucose level. Genetic testing revealed a new variation of ABCC8 mutation. Therefore, we report this case of CHI caused by a novel mutation of ABCC8 in a half-Korean newborn infant with diazoxide-unresponsive hyperinsulinemic hypoglycemia.

Lymphatic flow and lymph nodes mapping method with indocyanine green fluorescence imaging in clinically advanced gastric cancer

Ji-Hyeon Park,Felix Berlth,Chaojie Wang,Jong-Ho Choi,Shin-Hoo Park,Yun-Suhk Suh,Seong-Ho Kong,Do-Joong Park,Hyuk-Joon Lee,Yoon Jin Kwak,Woo Ho Kim,Han-Kwang Yang 대한종양외과학회 2020 대한임상종양학회 학술대회지 Vol.2020 No.7

Feasibility and safety of inserting self-biodegradable stents in the pylorus during pylorus-preserving gastrectomy for gastric cancer: A study in porcine for proof of concept

Ji-Hyeon Park,Hyesung Yoon,Yoon Jin Kwak,Chaojie Wang,Khalid Mohammed Alzahrani,Sen Wang,Fadhel Dhaifallah H. Alzahrani,Hyun Myong Kim,Eunhee Koo,Jaeun Yoo,Jong-Ho Choi,Shin-Hoo Park,Seong-Ho Kong,Do 대한종양외과학회 2022 대한임상종양학회 학술대회지 Vol.2022 No.2