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JeongEun HYEON 한국생물공학회 2021 한국생물공학회 학술대회 Vol.2021 No.10
In the practice of converting biomass into valuable biomaterials, the critical step is the decomposition process to give fermentable monomeric sugars. Thus, the designed microbes based on enzyme complexes are a key biological technology for biorefinery. For utilizing of polysaccharides by simultaneous saccharification and fermentation, a recombinant scaffolding protein from Clostridium cellulovorans and chimeric hydrolysis enzymes were assembled as complex system. The utilization of scaffolds for enzyme immobilization involves advanced bionanotechnology applications in biorefinery fields, which can be achieved by optimizing the function of various enzymes. The assembly of minicellulosomes by Saccharomyces cerevisiae and Corynebacterium glutamicum increased the activity against various lignocellulosic materials by approximately 3-fold compared with control. In addition, red algae-degrading complexes increased the activity against the marine biomass substrate by approximately 2-fold. Final, Carbon monoxide (CO) was successfully converted by functional complexes containing carbon monoxide dehydrogenase and carbon monoxide sensing heme protein with enhanced CO binding affinity. An enzyme complex was anchored on the cell surface of CO2-utilizing Ralstonia eutropha and successfully showed 3.3-fold increased conversion efficiency. Intelligent application of various scaffolds to couple with nanoscale engineering tools and metabolic engineering technology may offer particular benefits. The development of multi-functional protein complexes for use as tools in whole-cell biocatalyst systems has drawn considerable attention as an attractive strategy for bioprocess applications.
Lee, Jeongeun,Rhee, Minhee,Min, Taek Ki,Bang, Hae In,Jang, Mi-Ae,Kang, Eun-Suk,Kim, Hee-Jin,Yang, Hyeon-Jong,Pyu, Bok Yang The Korean Pediatric Society 2016 Clinical and Experimental Pediatrics (CEP) Vol.59 No.no.sup1
X-linked agammaglobulinemia (XLA) is a hereditary humoral immunodeficiency that results from Bruton's tyrosine kinase (BTK ) gene mutations. These mutations cause defects in B-cell development, resulting in the virtual absence of these lymphocytes from the peripheral circulation. Consequently, this absence leads to a profound deficiency of lg all isotypes, and an increased susceptibility to encapsulated bacterial infections. A 15-month-old Korean boy presented with recurrent sinusitis and otitis media after 6 months of age, and had a family history of 2 maternal uncles with XLA. Laboratory tests revealed a profound deficiency of Ig isotypes, and a decreased count of $CD19^+$ B cells in the peripheral circulation. Based on his family history and our laboratory test results, he was diagnosed with XLA. We performed BTK gene analysis of peripheral blood samples obtained from family members to confirm the diagnosis. Mutational analysis revealed a novel hemizygous frameshift mutation (c.82delC, p.Arg28Alafs*5), in the BTK gene. His mother and maternal grandmother were heterozygous carriers of this mutation and his two maternal uncles were hemizygous at the same position. After XLA diagnosis, intravenous immunoglobulin (400 mg/kg, monthly) treatment was initiated; recurrent sinusitis and otitis media were subsequently brought under control. To our knowledge, this is the first reported case of a Korean pedigree with a novel mutation in the BTK gene.
Increased Binding Efficiency of Enzyme Complex Based on Biomimicry Technology of Microorganisms
Habin SUN,JeongEun HYEON 한국생물공학회 2021 한국생물공학회 학술대회 Vol.2021 No.10
Protein-protein interaction (PPI) has been studied in many ways and from many perspectives, leading to advances in biological functional control. This has also led to the study of scaffold protein, which have a very important role as regulators in signalling, and the development of extracellular multi-enzyme complexes using weak interactions, such as ionic bonding. Here we studied the manufacture of other artificial scaffolding proteins to replace this scaffold protein, and in the process we used two peptides and performed overlap PCR to bind the two DNA. This experiment, which is changed in a typical PCR, allows the primer to be configured to have a 5’ overhang complementary at the end of another ㏖ecule so that the DNA after annealing is expanded. When the two DNA ㏖ecules are expanded in this way, they are mixed and a PCR is carried out with only the primers for the far ends. In the course of the study, two peptide-linked genes were obtained, which produced covalent bond-based proteins that could replace conventional non-covalent bonds. This will bring structural stability to the enzyme complex, providing benefits that do not change easily in the heat.
YouKyeong LEE,JeongEun HYEON 한국생물공학회 2021 한국생물공학회 학술대회 Vol.2021 No.10
In the food industry, interest in and consumption of health functional foods for antioxidant and immunity enhancement is increasing significantly due to the effects of aging and the novel coronavirus (COVID-19). Therefore, research related to functional substances is also being actively conducted. Astaxanthin is a ketocarotenoid-type red pigment and has been investigated as an antioxidant that is about 10 times more powerful than other types of carotenoids. The chemical compound form of astaxanthin has problems with lower bioavailability and lower stability than natural extracts. In this study, an enzyme used for Xanthophyllomyces dendrorhous was developed through a transformed microorganism and the enzyme ability was evaluated through halo assay and DNS method. In addition, astaxanthin extracted from X. dendrorhous were analyzed for astaxanthin using HPLC. As a result, enzyme production was successfully performed from the transformed microorganism, and the enzymatic activity of the prepared enzyme was confirmed qualitatively and quantitatively. Through these studies, it is expected that it can be directly applied to the functional food market containing astaxanthin extracted from X. dendrorhous.