Rat의 복강에 삽입한 Filorouracil-Polyglycolic acid제형의 Fluorouracil용출에 관한 연구

노승무,정경수,오정연,김진향,양준묵,강대영,송규상,최정목,최선웅,이진호,조준식,민병무,김용백,김창식,박근성,김승영,김학용,인현빈 충남대학교 의과대학 지역사회의학연구소 1998 충남의대잡지 Vol.25 No.1

A common form of relapse in adenocarcinoma of the stomach is intraperitioneal dissemination, in fact, among gastric adenocarcinoma patients who have undergone surgery intended to cure, approximately 50% of the patients develope initial recurrence in the peritoneal cavity regardless of the anatomic site of the primary tumor within the stomach. The efficacy of systemic postoperative chemotherapy to prevent peritoneal recurrence of gastrric adenocarcinoma is not satisfactory. There is still a great need for improved therapeutic strategies on the disseminated microscopic disease and small miliary nodules remaining on the peritoneal surface or lymphatics after operation. The authers have made fluorouracil-polyglycolic acid composite disks(Fu-PGA disks) with fluorouracil and biodegradable polymer: polyglycolic acid for more effective intraperitoneal chemotherapy. We inserted the Fu-PGA disk(s) in the peritoneal cavity of rat and pharmacokinetic study was performed to measure fluorouracil concentration in the peritoneal fluid, plasma, liver, kidney and heart tissue at 24 hour, 72 hour and 168 hour after insertion of Fu-PGA disk(s). Myelosuppressive action of this composite also was determined following its administration. The data of this study suggested that Fu-PGA composite will be a new device releasing drugs in a controlled manner and having targetability to peritoneum, and this device will be improving the efficacy of intraperitoneal chemotherapy for gastric adenocarcinoma.

Rat의 복강에 삽입한 Fluorouracil-Polyglycolic acid 제형의 Fluorouracil 용출에 관한 연구

노승무,정경수,오정연,김진향,양준묵,강대영,송규상,최정목,최선웅,이진호,조준식,민병무,김용백,김창식,박근성,김승영,김학용,인현빈 忠南大學校 癌共同硏究所 1998 癌共同硏究所 硏究誌 Vol.2 No.1

A common form of relapse in adenocarcinoma of the stomach is intraperitoneal dissemination, in fact, among gastric adenocarcinoma patients who have undergone surgery intended to cure, approximately 50% of the patients develope initial recurrence in the peritoneal cavity regardless of the anatomic site of the primary tumor within the stomach. The efficacy of systemic postoperative chemotherapy to prevent peritoneal recurrence of gastrric adcnocarcinoma is not satisfactory. There is still a great need for improved therapeutic strategies on the disseminated microscopic disease and small miliary nodules remaining on the peritoneal surface or lymphatics after operation. The authers have made fluorouracil-polyglycolic acid composite disks(Fu-PGA disks) with fluorouracil and biodegradable polymer: polyglycolic acid for more effective intraperitoneal chemotherapy. We inserted the Fu-PGA disk(s) in the peritoneal cavity of rat and pharmacokinetic study was performed to measure fluorouracil concentration in the peritoneal fluid, plasma, liver, kidney and heart tissue at 24 hour, 72 hour and 168 hour after insertion of Fu-PGA disk(s). Myelosuppressive action of this composite also was determined following its administration. The data of this study suggested that Fu-PGA composite will be a new device releasing drugs in a controlled manner and having targetability to peritoneum, and this device will be improving the efficacy of intraperitoneal chemotherapy for gastric adenocarcinoma.

V₂O-B₂O₃-PbO 유리의 전자 상자성 공명연구

노태호,전인,박영민,강재필,김성재,박현진,송승기 명지대학교 자연과학연구소 1996 자연과학논문집 Vol.14 No.-

V₂O??-B₂O₃-PbO 유리의 VO??에 관한 전자 상자성 (EPR) 스펙트럼으 연구하였으며, vanadium이온의 스핀해밀토니안 상수들을 구하였다. 유리시료안의 V₂O??양을 증가시킴에 따라 g??과 g??값은 증가하였으나 A??과 A??값은 감소하였다. 이는 V₂O??수가 증가하면 동일 평면상의 vanadium과 oxygen배위간 결합의 공유성이 증가함을 의미한다. 유리내의 vanadium 4가이온은 정방형 일그러진 8면체를 한 vanadyl착물로 존재한다. Electron Paranmagnetic Resonance(EPR) SPECTRA OF (VO)?? in V₂O??-B₂O₃-PbO glasses have been studied and spin hamiltonian paramenters of vanadium ion have been calculated. It is found that the value of g??and g?? increases while the value of A?? and A?? decreases with an increase of V₂O?? content in the glass samples, showing that the covalency in the in-plane vanadium -oxygen ligand bonds increase with increasing the concentration of V₂O??. The tetravalent vanadium ions exist as vanadyl complexes with tetragonally distorted octahedral sites in the glasses.

감마선 조사가 조미오징어의 저장 중 품질특성에 미치는 영향

노정은,김병근,김현구,권중호 한국식생활문화학회 2004 韓國食生活文化學會誌 Vol.19 No.5

This study was conducted to investigate the changes in the quality of gamma-irradiated seasoned cuttle during storage. Seasoned cuttle packed in PVC film (0.06 mm) was stored at 15±1℃ for 6 months after treatment with doses of 0 to 7 kGy. Microbial populations of seasoned cuttle were 1.6×10^5 CFU/g in total aerobic bacteria, 10⁴~10^5 CFU/g in yeasts & molds, and negative in coliforms, which were effectively reduced by 3 kGy or higher up to the undetectable level(〈20 CFU/g). The pH and moisture content of the samples were not changed with irradiation, but moisture was some decreased during storage. The instrumental color (especially Hunter b value), pigments (lipophilic & hydrophilic) and TBA value of the samples increased with storage time as well as irradiation dose more than 3 kGy. The influence of storage condition, however, were more significant. Irradiation did not induce any changes in volatile basic nitrogen (VBN) and trimethylamine (TMA) contents, thereby maintaining their contents lower than those of the non-irradiated samples during storage by reducing the microbial load.

위암환자의 복강내에 투여한 Mitomycin C-Carbon Particle의 Mitomycin 용출에 관한 연구

노승무,조영훈,정경수,오정연,김진향,양준묵,강대영,송규상,조준식,최선웅,이진호,민병무,김용백,김창식,박근성,인현빈,정현용,김학용 충남대학교 의과대학 지역사회의학연구소 1998 충남의대잡지 Vol.25 No.1

Locoregional recurrence is the most common type of recurrence in surgical operation of gastric adenocarcinoma, and peritoneal dissemination is one of the most difficult problems in advanced gastric adenocarcinoma treatment. Because the peritoneal cavity is the most common site of the first recurrence after gastric cancer resection, intraperitoneal chemotherpy seems a logical choice for cancer chemotherapy. The Mitomycin C(MMC) adsorbed by the activated charcoal particles(CH) is relatively released when the drug concentration surrounding the carbon particles becomes low in the peritoneum of the peritoneal cavity. For the intraperitoneal chemotherapy on the advanced gastric adenocarcnoma, mitomycin C adsorbed on activated carbon particles was administered in the peritoneal cavity just before abdominal wall closure. The closed drainage tubes were inserted in the peritoneal cavity and clamped for tuo hours after completion of operation. MMC concentrations were serially measured in peritoneal fluid, plasma and urine at 2hour, 48 hour, 72 hour and 168 hour following its administration in order to study the efficacy of the MMC-CH as a drug delivery system. There were minimal toxicities in born marrow, liver, and gastrointestinal system after intraperitoneal MMC-CH administration. The data of this study suggested that MMC-CH may have a somewhat more beneficial effect than surgery alone when administered in optimal dose and schedules, but the MMC concentration of the peritoneal fluid was not sufficient to eradicate remnant cancer cells, and effective duration of maintenance was only below 24 hours in the peritoneal fluid and plasma.

수종 한약재가 백혈병 세포에 미치는 영향에 대한 연구

崔湖榮,金澤,柳志昌,卞德市,盧昇鉉 대한본초학회 1999 大韓本草學會誌 Vol.14 No.1

I am reporting here a syntematic in vitro examination for specificity of anti-cancer effect of several oriental herbs, which have been used frequently for cancer pateints(therapy), on Molt-4 LCL(Lymphoblastoid Cell Line) Leukemic cells in relation to its density and time of incubation. 1. ErOH extract of Jujubae Fructus reduced Molt-4 cell's survival rate effectively after 48 hours' treatment at 1000㎍/㎖'s density and it reduced survival rate of LCL effectively after 12 hours' treatment at 1000㎍/㎖'s density. 2. EtOH extract of Cordyceps reduced LCL's survival rate effectively after 36 hour's treatment at 1000㎍/㎖'s density. 2. EtOH extract of Fici Fructus reduced LCL's survival rate effectively after 36 hours' treatment at 1000㎍/㎖'s density. 4. EtOH extract of Trionycis reduced Molt-4 cell's survival rate even at 500㎍/㎖ density and further effective at 1000㎍/㎖ density after 24 hours treatment and it killed the cells completely at 1000㎍/㎖ density after 48 hours treatment, It also Killed LCL completely after 12 hours and 36 hours' treatment at 1000㎍/㎖'s density. 5. EtOH extract of Biotae Cacumen reduced LCL's survival rate effectively after 12 hours' treatment at 1000㎍/㎖'s density, and after 36 hours treatment it reduced LCL's survival time very effectively. 6. EtOH extract of Psoraleae Fructus reduced Molt-4 cell's survival rate very effectively after 24 hours' treatment at 500㎍/㎖ and 1000㎍/㎖'s density, and after 48 hours' treatment at 250㎍/㎖'s density it reduced effectively and it killed the cell completely at 500㎍/㎖ and 1000㎍/㎖'s density. It also killed LCL completely after 12 hours and 36 hours' treatment at 1000㎍/㎖'s density. I hope to proceed with more advanced experiment in vivo and clinically as for the two kinds of oriental herbs and another herbs effective for leukemia.

수복용복합레진의 표면처리방법에 따른 표면중합률 및 변색정도의 변화와 그 상관관계에 대한 연구

박성호,김모란,노병덕,안현정 대한치과보존학회 2000 Restorative Dentistry & Endodontics Vol.25 No.3

The purpose of the present study was first, to evaluate the relationship between composite surface conversion and surface discoloration, second. to know if there was difference in surface discoloration between celluloid-strip-finished composite surface and polished surface. In addition, the discoloration of composite surface was also evaluated with visual inspection or digital camera with high resolution monitor. Z-100 Tetric Ceram. Spectrum. and Aelitiril were used. The composite surfaces were celluloid-strip finished (group1) , polished (group 2) . celluloid- strip finished under nitrogen gas purging (group 3) or only light cured without finishing or polishing under nitrogen gas purging (group 4) . The microhardness of each samples were also measured in each group, The samples of each group were also divided into 4 subgroup whether they were immediately placed in disclosing solution (0.2% Erythrosin, pH 7.0) (subgroup1 ) . l day after light curing(subgroup 2 ) .3day after light rulings (subgroup3) or 7 day after light curing(subgroup4). The computer controlled spectrophotometer was used to determine CIELAB coordinates (L*, a * , b*) The amounts of color difference were compared. The results were as follows: 1.There was no difference in discoloration between celluloid strip finished composite surface and polished surface 2.The samples discolored more when they were placed in disclosing solution immediately after polymerization than other groups. 3.When the samples were light cured under nitrogen gas purging and without polishing process. they discolored more than other groups even through they showed higher microhardness. 4.With visual inspection or digital camera. only a limited information was available in detecting composite surface discolation

위암 환자의 복강내 투여를 위한 Activated Charcoal-Alginate Bead 제형으로부터 Mitomycin C의 용출 거동

이진호,최선웅,서중기,김동민,정경수,오정연,김진향,노승무,민병무,김용백,김창식,박근성,강대영,송규상,양준묵,조준식,정현용,김학용,인현빈 충남대학교 의과대학 지역사회의학연구소 1998 충남의대잡지 Vol.25 No.1

Locoregional recurrence is the most common type of recurrence in surgical operation of gastric adenocarcinoma, and peritoneal dissemination is one of the most difficult problems in advanced gastric adenocarcinoma treatment. Because the peritoneal cavity is the most common site of the first recurrence after gastric cancer resection, intraperitoneal chemotherapy seems a logical choice for cancer chemotherapy. In this study, Mitomycin C (MMC)-activated charcoal (CH)-alginate (ALG) beads were prepared by the mixtures of CH particles adsorbed with MMC as an anti-cancer drug and aqueous alginate solution. The alginate is recognized as biodegradable, nontoxic, and biocompatible. The release of MMC from the beads in 0.1 M Tris buffer was stable and continuous until about 1 week. The MMC-CH-ALG beads can be applied in the peritoneal cavity for intraperitoneal chemotherapy since they provide a good adhesiveness on the tissue and controlled release pattern of the drugs.

LED를 이용한 복합레진의 광조사시, 중합수축의 속도와 양, 미세경도에 관한 연구

박성호,김수선,조용식,이순영,김도현,장용주,문현승,서정원,노병덕 大韓齒科保存學會 2003 Restorative Dentistry & Endodontics Vol.28 No.4

This study evaluated the effectiveness of the light emitting diode(LED) units for composite curing. To compare its effectiveness with conventional quartz tungsten halogen (QTH) light curing unit, the microhardness of 2mm composite, Z250, which had been light cured by the LEDs (Ultralume LED2, FreeLight, Developing product D1) or QTH (XL 3000) were compared on the upper and lower surface. One way ANOVA with Tukey and Paired t-test was used at 95% levels of confidence. In addition, the amount of linear polymerization shrinkage was compared between composites which were light cured by QTH or LEDs using a custom-made linimeter in 10s and 60s of light curing, and the amount of linear polymerization shrinkage was compared by one way ANOVA with Tukey. The amount of polymerization shrinkage at 10s was XL3000 > Ultralume 2, 40, 60 > FreeLight, D1 (P<0.05) The amount of polymerization shrinkage at 60s was XL3000 > Ultralume 2, 60 > Ultralume 2, 40 > FreeLight, D1 (P<0.05) The microhardness on the upper and lower surface was as follows; <본문 참조> It was concluded that the LEDs produced lower polymerization shrinkage in 10s and 60s compared with QTH unit. In addition, the microhardness of samples which had been cured with LEDs was lower on the lower surfaces than the upper surfaces whereas there was no difference in QTH cured samples.

포름알데히드 含有 廢水가 嫌氣性 處理에 미치는 影響

박광석,노현웅,박정호,박승조 동아대학교 환경문제연구소 1997 硏究報告 Vol.20 No.1

The effects of formaldehyde in anaerobic digestion were : 1. In the cases of batch assay, the methanogenic bacteria were tolerated the toxic when raw and pretreated wastewaters were injected 3 and 5 ml respectively to serum bottle, but shocked when 5 and 10 ml respectively. 2. When raw, pretreated + glucose, and pretreated wastewaters were applied to UASB reators, the results of VLR were obtained 8-10, 3-5, and 2-4 kg cod/㎥ ·d respectived. Result of raw wastewater was the most optima of three. 3. The correlations VLR with bio-gas were obtained 90.5, 46.4 and 34.6% respectively in raw, pretreated + glucose, and pretreated wastewaters. Result of raw wastewater was the most optima of three. 4. In batch assay, pretreatment wastewater is better than raw water, but in continuous assay, raw wastewater is better than pretreatment wastewater. This reason is consideated that unsoluble salt of 373 mg/l is accumulated within UASB reactor, and carries out inhibition to anaerobic bacteria.