여름철 계룡산국립공원 탐방로의 열쾌적성 및 어메니티 관리 연구

안시옥(Siok An),송도윤(Doyun Song),정다워(Dawou Joung),박범진(Bum-Jin Park),심규원(Kyu-Won Sim),박준효(Jun-Hyo Park) 국립공원연구원 2022 국립공원연구지 Vol.13 No.2

본 연구는 산악환경의 기상 특징과 계룡산국립공원 탐방로의 열쾌적성을 파악하고 열쾌적성이 높은 활동 시간 정보, 활동 공간 정보, 활동량 정보를 제공하기 위해 시행되었다. 탐방로의 열쾌적성 분석을 위해 여름철 도심 기온, 국립공원 방재기상관측장비(AWS, Automatic Weather System), 탐방로인 숲길의 평균 기온을 비교하였다. 또한, 계룡산 국립공원의 주요 탐방로 4개 구간의 미기후 요소와 수관울폐도 측정을 위해 총 240개 지점을 조사 후 열쾌적성을 파악 하였다. 도심과 국립공원AWS, 탐방로의 평균 기온을 비교하였을 때, 도심, 갑사AWS, 동학사AWS, 갑사 탐방로, 동학사 탐방로 순으로 평균 기온이 높게 나타났다. 이 결과, 탐방객이 직접 체감하는 탐방로의 열쾌적성을 고려해야 함을 의미한다. 주요 탐방로 4개 구간 조사 결과, 평균 수관울폐도는 동학사~남매탑 구간, 동학사~관음봉 구간, 갑사~연천봉 구간, 갑사~금잔디고개 구간 순으로 높았다. PMV(예상평균온열감)는 활동량이 2.8MET 일 때, 갑사~금잔디고개 구간, 갑사~연천봉 구간, 동학사~남매탑, 동학사~관음봉구간 순으로 높았다. 조사를 통하여, 열쾌적성이 높은 시간 정보, 공간 정보, 활동량 정보를 제시할 수 있었다. 탐방로의 열쾌적성 특성을 파악하여, 탐방로의 어메니티를 높일 수 있는 숲길 관리 방안의 기초를 마련하고자 한다. This study was conducted to understand the weather characteristics of the mountain environment and the thermal comfort of Gyeryongsan National Park trail and to provide information on activity time, activity space, and activity amount. For the analysis of the thermal comfort of the trail, the temperature of the city center in summer, the average temperature of the National Park Disaster Prevention Weather System (AWS), and the trail. Also, Microclimate elements and canopy closure were also measured in four major trails in Gyeryongsan National Park. After investigating a total of 240 points, thermal comfort was identified. When comparing the average temperature of the city center, National Park AWS, and trail, the average temperature was higher in the order of the city center, Gabsa AWS, Donghaksa AWS, Gabsa Trail, and Donghaksa Trail. As a result, it means that the thermal pleasure experienced by visitors should be considered. According to a survey of four major trails, the average canopy closure is Donghaksa~nammaetop route was high in the order of Donghaksa~Gwaneumbong route, Gabsa~Yeoncheonbong route and Gabsa~Geumjandigogae route. When PMV(Predicted Mean Vote) is 2.8MET, The PMV was highter in the order of Gabsa~Geumjandigogae route, Gabsa~ Yeoncheonbong route, Donghaksa~nammaetop route, Donghaksa~Gwaneumbong route. Through the survey, time information, spatial information, and activity information with high thermal comfort could be presented. By grasping the thermal comfort characteristics of the trail, we intend to lay the foundation for forest trail management measures that can increase the amenity of the trail.

Regulation of the Endoplasmic Reticulum Stress by BIP/GRP78 is involved in Meiotic Maturation of Porcine Oocytes In Vitro

Hyo-Jin Park,Jae-Young Park,Jin-Woo Kim,Seul-Gi Yang,Jae-Min Jung,Min-Ji Kim,Joung Jun Park,Deog-Bon Koo 한국발생생물학회 2017 발생과 생식 Vol.21 No.4

In the present study, we investigated the role of binding immunoglobulin protein/glucose-regulated protein, 78-kDa (BIP/GRP78)-regulated endoplasmic reticulum (ER)-stress on meiotic maturation and cumulus cells expansion in porcine cumulus-oocyte complexes (COCs). Previously, it has been demonstrated that unfolded protein response (UPR)- related genes, such as molecules involved in ER-stress defense mechanisms, were expressed in matured oocytes and cumulus cells during in vitro maturation (IVM) of porcine oocytes. However, BIP/GRP78-mediated regulation of ER stress in porcine oocytes has not been reported. Firstly, we observed the effects of knockdown of BIP/GRP78 (an UPR initiation marker) using porcine-specific siRNAs (#909, #693, and #1570) on oocyte maturation. Among all siRNAs, siRNA #693 significantly reduced the protein levels of UPR marker proteins (BIP/GRP78, ATF4, and P90ATF6) in porcine COCs observed by Western blotting and immunofluorescence analysis. We also observed that the reduction of BIP/GRP78 levels by siRNA#693 significantly inhibited the meiotic maturation of oocytes (siRNA #693: 32.5±10.1% vs control: 77.8±5.3%). In addition, we also checked the effect of ER-stress inhibitors, tauroursodeoxycholic acid (TUDCA, 200 μM) and melatonin (0.1 μM), in BIP/ GRP78-knockdown oocytes. TUDCA and melatonin treatment could restore the expression levels of ER-stress marker proteins (BIP/GRP78, p-eIF2α, eIF2α, ATF4, and P90ATF6) in siRNA #693-transfected matured COCs. In conclusion, these results demonstrated that BIP/GRP78-mediated regulation of UPR signaling and ER stress plays an important role in in vitro maturation of porcine oocytes.

Melatonin Reduces the Bisphenol A-induced Superoxide and Improves Oocyte Maturation through Reducing of Mitochondrial Derived Apoptosis in Pigs

Hyo-Jin Park,Jin-Woo Kim,Seul-Gi Yang1,Min-Ji Kim,Ho-Guen Jegal,Joung Jun Park,Deog-Bon Koo 한국동물생명공학회(구 한국동물번식학회) 2018 발생공학 국제심포지엄 및 학술대회 Vol.2018 No.06

Bisphenol-A (BPA) as an organic synthetic compound of exhibiting estrogen-mimicking and hormone-like properties, which is commonly used to induce cellular stress or female reproductive toxicity. In addition, BPA induces the increasing of mitochondrial derived reactive oxygen species (ROS) such as superoxide, and production of these ROS affects to the meiotic maturation and cumulus cells expansion on in vitro maturation (IVM) of porcine cumulus-oocyte complexes (COCs). However, anti-oxidative effect of melatonin for reduction of BPA-induced superoxide on porcine oocyte maturation has not been reported. Therefore, in present study, we confirmed that the reduction of BPA-derived superoxide by melatonin related to the reducing of mitochondria mediated apoptosis on meiotic maturation and cumulus cells expansion of porcine COCs. Then, to investigate the effects of superoxide specific scavenger, Mito-TEMPO, during porcine oocyte maturation progression, COCs cultured in maturation medium with Mito-TEMPO (0.1 μM) after pre-treatment of BPA (75 μM) for 22 h. Reduced meiotic maturation rate and cumulus cells expansion of COCs in the BPA (75 μM) treated group were recovered (p<0.01) by Mito-TEMPO treatment. Also, increasing of mitochondria derived apoptotic factors (AIF, Cleaved Caspase 3 and Cleaved PARP 1) protein levels by BPA treatment were reduced by Mito-TEMPO treatment in porcine COCs maturation. Positive effects of Mito-TEMPO for superoxide reduction on oocyte maturation and reducing mitochondrial apoptosis showed the same pattern in melatonin (0.1 μM) treated COCs. In case of supplemented with BPA and melatonin, superoxide production in COCs was not changed compared to control or melatonin treated groups. Based on these results, we concluded that melatonin as a regulator of superoxide such as Mito-TEMPO improves oocyte maturation through reduction of mitochondria derived apoptosis during IVM of porcine COCs.

Clinical Practice Guidelines for Managing Frailty in Community-Dwelling Korean Elderly Adults in Primary Care Settings

Hyo-Sun You,Yu-Jin Kwon,Sun Young Kim,Yang Hyun Kim,Ye-Seul Kim,Yonghwan Kim,노용균,Byoungjin Park,Young-Kyu Park,Chang-Hae Park,Joung Sik Son,Jinyoung Shin,Hyun-Young Shin,Bumjo Oh,이재우,Jae-YongShim,Chan 대한가정의학회 2021 Korean Journal of Family Medicine Vol.42 No.6

Aging has become a global problem, and the interest in healthy aging is growing. Healthy aging involves a focus on the maintenance of the function and well-being of elderly adults, rather than a specific disease. Thus, the management of frailty, which is an accumulated decline in function, is important for healthy aging. The adaptation method was used to develop clinical practice guidelines on frailty management that are applicable in primary care settings. The guidelines were developed in three phases: preparation (organization of committees and establishment of the scope of develop-ment), literature screening and evaluation (selection of the clinical practice guidelines to be adapted and evaluation of the guidelines using the Korean Appraisal of Guidelines for Research and Evaluation II tool), and confirmation of recom-mendations (three rounds of Delphi consensus and internal and external reviews). A total of 16 recommendations (five recommendations for diagnosis and assessment, 11 recommendations for intervention of frailty) were made through the guideline development process. These clinical practice guidelines provide overall guidance on the identification, evalua-tion, intervention, and monitoring of frailty, making them applicable in primary care settings. As aging and “healthy ag-ing” become more and more important, these guidelines are also expected to increase in clinical usefulness.

Regulation of the Endoplasmic Reticulum Stress by BIP/GRP78 is involved in Meiotic Maturation of Porcine Oocytes In Vitro

Park, Hyo-Jin,Park, Jae-Young,Kim, Jin-Woo,Yang, Seul-Gi,Jung, Jae-Min,Kim, Min-Ji,Park, Joung Jun,Koo, Deog-Bon The Korean Society of Developmental Biology 2017 발생과 생식 Vol.21 No.4

In the present study, we investigated the role of binding immunoglobulin protein/glucose-regulated protein, 78-kDa (BIP/GRP78)-regulated endoplasmic reticulum (ER)-stress on meiotic maturation and cumulus cells expansion in porcine cumulus-oocyte complexes (COCs). Previously, it has been demonstrated that unfolded protein response (UPR)-related genes, such as molecules involved in ER-stress defense mechanisms, were expressed in matured oocytes and cumulus cells during in vitro maturation (IVM) of porcine oocytes. However, BIP/GRP78-mediated regulation of ER stress in porcine oocytes has not been reported. Firstly, we observed the effects of knockdown of BIP/GRP78 (an UPR initiation marker) using porcine-specific siRNAs (#909, #693, and #1570) on oocyte maturation. Among all siRNAs, siRNA #693 significantly reduced the protein levels of UPR marker proteins (BIP/GRP78, ATF4, and P90ATF6) in porcine COCs observed by Western blotting and immunofluorescence analysis. We also observed that the reduction of BIP/GRP78 levels by siRNA#693 significantly inhibited the meiotic maturation of oocytes (siRNA #693: $32.5{\pm}10.1%$ vs control: $77.8{\pm}5.3%$). In addition, we also checked the effect of ER-stress inhibitors, tauroursodeoxycholic acid (TUDCA, $200{\mu}M$) and melatonin ($0.1{\mu}M$), in BIP/GRP78-knockdown oocytes. TUDCA and melatonin treatment could restore the expression levels of ER-stress marker proteins (BIP/GRP78, $p-eIF2{\alpha}$, $eIF2{\alpha}$, ATF4, and P90ATF6) in siRNA #693-transfected matured COCs. In conclusion, these results demonstrated that BIP/GRP78-mediated regulation of UPR signaling and ER stress plays an important role in in vitro maturation of porcine oocytes.

광용적맥파를 이용한 수면 저호흡 검출 가능성 평가를 위한 예비연구

박종욱(Joung-Uk Park),에르덴바야르(Urtnasan Erdenebayar),이효기(Hyo-Ki Lee),이경중(Kyoung-Joung Lee) 대한전기학회 2014 대한전기학회 학술대회 논문집 Vol.2014 No.2

Glomosim을 이용한 X-MAC 시뮬레이터 구현 및 성능 평가

김효중(Hyo-Joung Kim),박만규(Man-Kyu Park),김병철(Byung-Chul Kim),이재용(Jea-Yong Lee),안재민(Jea-Min Ahn),김세한(Se-Han Kim),박태준(Tae-Jun Park) 한국통신학회 2007 한국통신학회 학술대회논문집 Vol.2007 No.11

증례 : Capecitabine에 의해 유발된 간질성 폐질환 1예

최효경 ( Hyo Kyung Choi ),김효송 ( Hyo Song Kim ),박혜윤 ( Hye Yun Park ),장혜원 ( Hye Won Jang ),정만표 ( Man Pyo Chung ),한정호 ( Joung Ho Han ),박준오 ( Joon Oh Park ) 대한내과학회 2006 대한내과학회지 Vol.71 No.1S

기야 이방운의 《정미년(丁未年) 사계산수도첩(四季山水圖帖)》 연구

박효정(Park Hyo-Joung) 명지대학교 문화유산연구소 2016 미술사와 문화유산 Vol.4 No.-

기야(箕埜) 이방운(李昉運)은 조선후기의 많은 문인화가들 가운데서 단아하고 참신한 작품을 많이 남기고 있어 관심과 주목을 받아왔다. 생애와 출신에 대해서는 별로 알려진바 없으나 상당수의 작품이 전하고 있고, 다양한 장르의 회화를 두루 섭렵하였다. 특히 중국의 옛 시를 화제로 삼은 ‘시의도(詩意圖)’를 많이 남겼다. 《정미년(丁未年) 사계산수도첩(四季山水圖帖)》은 기야 이방운이 정미년(1787)에 화론을 쓰고, 그 보다 몇 해 전에 그린 그림들로 구성되어 있다. 연대가 확실하고, 무엇보다 이방운의 화론이 실려 있어 회화사에서도 귀중한 사료로 주목 된다. 첩의 그림들 대부분은 조선후기 화가들 사이에서 많이 그려진 시의도로서 옛 시의 의취에 따라 계절별로 꾸민 사계산수도의 형식을 띤다. 이 첩은 모두 9면으로 구성되어 있고, 그림은 총 10점이 실려 있다. 작품의 화제는 모두 당ㆍ송ㆍ명대의 시에서 계절감을 나타내는 문장을 부분적으로 차용하여 쓰고, 화제와 어울리는 그림을 그렸다. 화첩 마지막 면은 이방운이 직접 짓고 쓴 화론으로서, ‘화사가 육법(六法)을 갖추고 하나로 아우를 수 있다면 당세뿐만 아니라 다른 세대에 전해지면서 영원토록 보배가 될 것이다’라고 하였다. 그 중 현재 심사정을 일컬어 ‘여러 품격을 갖추어 이름난 화가’임을 말하고 있는데, 그동안 이방운과 심사정 화풍의 영향관계를 보다 확신 할 수 있게 되었다는 점에서 큰 성과라 볼 수 있다. 또 이방운은 그림의 배포(포치)를 당시화보를 따르고 있다고 하였다. 그러나 화면을 자의적으로 변용하였고, 자신이 이룩한 화풍으로 독창적인 시의도를 그렸다. 이유원의 『임하필기(林下筆記)』에는 이방운의 가장 후기의 삶이 기록되어 주목된다. 첫째, 이방운이 최소 63세 이상까지는 생존했을 것으로 가정해볼 수 있다. 둘째, 과거 시험을 대신 봐줬을 정도로 학자적 기본 소양과 학식을 갖추고 있었음을 짐작할 수 있다. 마지막으로, 사람들에게 ‘광생’이라 불렸다는 점은 틀에 얽매이지 않고 자유분방한 성격의 소유자였음을 짐작할 수 있다. 각 그림들의 전체적인 분위기는 소략하면서도 유약한 필선과 담채 등을 사용하여 사의적(寫意的)경향이 두드러진다. 또한 이방운은 시와 글씨에도 뛰어나 시서화 일치의 면모를 잘 보여주고 있다. 20대 초반의 그림이라고 하기에 필치가 세련되고, 담채의 사용이 맑고 능숙하다. 당시화보와 강세황의 구도를 차용함에 있어서는 개성적으로 재해석하여 매우 안정적이다. 다만 초년작이 갖는 미숙한 필법은 어쩔 수 없이 나타난다. 그럼에도 불구하고 정조부터 순조대까지 이어지고 있는 화원화가의 시대 속에서 문인화가 이방운이라는 화가는 새롭게 조명해 볼 필요가 있는 화가라고 생각된다.

유방암 생존자의 재발방지 행위와 삶의 질

민효숙(Min, Hyo Suk),박선영(Park, Sun Young),임정선(Lim, Joung Sun),박미옥(Park, Mi Ok),원효진(Won, Hyo Jin),김종임(Kim, Jong Im) 한국간호과학회 2008 Journal of Korean Academy of Nursing Vol.38 No.2