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CHEUN, Hyeng-Il,CHO, Shin-Hyeong,LIM, Yi-Young,LEE, Byung-Chul,KIM, Jung-Yeon,JU, Jung-Won,NA, Byoung-Kuk,KIMATA, Isao,YU, Jae-Ran,KIM, Tong-Soo Japanese Society of Veterinary Science 2010 The Journal of veterinary medical science Vol.72 No.2
<P>Cryptosporidiosis is a diarrheal illness caused by apicomplexa parasite <I>Cryptosporidium</I> spp. In this study, to examine the overall infection status of <I>Cryptosporidium</I> spp. in individuals residing in southern parts of Korea, eight counties around Yeongsan, Seomjin and Nakdong River valleys was surveyed. The investigation was carried out from April to October 2005. A total of 9,498 stool samples were collected from individuals. Stool samples were analyzed for modified acid-fast stains, and DNA fragment extracted from positive samples was identified by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) for 18S rRNA polymorphic region. Oocysts of <I>Cryptosporidium</I> spp. were detected in 239 specimens (2.5%) by a modified acid-fast stain. Infection rate was not significantly different between male (2.2%) and female (2.8%) individuals examined (<I>P</I>>0.05). In the infection rate by age, totally 1-9 (4.8%) and 80< (3.7%) age group were shown to the highest, and there was shown to significant differences (<I>P</I><0.05). Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) analysis of 18S rRNA gene from 51 isolates showed that all the isolates were identified as <I>C. parvum</I>. Our data collectively suggested that <I>C. parvum</I> infection is prevalent in the studied areas of Korea and more comprehensive nation-wide epidemiological studies are needed to elucidate the infection status of <I>Cryptosporidium</I> infection in Korea.</P>
Cryptosporidium hominis Infection Diagnosed by Real-Time PCR-RFLP
Hyeng-Il Cheun,Kyungjin Kim,Sejoung Yoon,Won-Ja Lee,Woo-Yoon Park,Seobo Sim,Jae-Ran Yu 대한기생충학열대의학회 2013 The Korean Journal of Parasitology Vol.51 No.3
There are approximately 20 known species of the genus Cryptosporidium, and among these, 8 infect immunocompetent or immunocompromised humans. C. hominis and C. parvum most commonly infect humans. Differentiating between them is important for evaluating potential sources of infection. We report here the development of a simple and accurate real-time PCR-based restriction fragment length polymorphism (RFLP) method to distinguish between C. parvum and C. hominis. Using the CP2 gene as the target, we found that both Cryptosporidium species yielded 224 bp products. In the subsequent RFLP method using TaqI, 2 bands (99 and 125 bp) specific to C. hominis were detected. Using this method, we detected C. hominis infection in 1 of 21 patients with diarrhea, suggesting that this method could facilitate the detection of C. hominis infections.
Young-Il Jeong,Hee-Eun Shin,Sang-Eun Lee,Hyeng-Il Cheun,Jung-Won Ju,Jung-Yeon Kim,Mi Yeoun Park,Shin-Hyeong Cho 대한기생충학열대의학회 2016 The Korean Journal of Parasitology Vol.54 No.2
Clonorchis sinensis is currently the most important parasite affecting public health problems in the Republic of Korea. We investigated the prevalence of C. sinensis infection among residents living along 5 major rivers in Korea. A total of 42,562 individual stool samples were collected from 37 localities and examined using the formalin-ether sedimentation technique. Helminth eggs were detected in 4,052 (9.5%) residents and 3,586 (8.4%) were infected with C. sinensis. The egg positive rate of C. sinensis in Nakdong, Seomjin, Geum, Yeongsan, and Han River was 11.7%, 9.9%, 6.5%, 3.1%, and 1.0%, respectively. The overall prevalence of clonorchiasis by sex was 11.2% in males and 6.2% in females. The age-prevalence was the highest in the 50-59 years band. It has been reconfirmed that the endemicity of clonorchiasis is higher in southern areas of Korea, especially along Nakdong and Seomjin Rivers. A combination of continuous control programs with health education initiatives is urgently required in these highly endemic areas of clonorchiasis in Korea.
Ju, Jung-Won,Joo, Hyun-Na,Lee, Myoung-Ro,Cho, Shin-Hyeong,Cheun, Hyeng-Il,Kim, Jung-Yeon,Lee, Young-Hee,Lee, Kwang-Jun,Sohn, Woon-Mok,Kim, Dong-Min,Kim, Il-Chul,Park, Byoung Chul,Kim, Tong-Soo WILEY-VCH Verlag 2009 Proteomics Vol.9 No.11
<P>Clonorchis sinensis, the Chinese liver fluke, is the causative agent of clonorchiasis as well as liver and biliary diseases. The excretory-secretory products (ESPs) of the parasites play important roles in host–parasite interactions. In this study, we have investigated the proteome of ESPs obtained from C. sinensis adult worms. Although the full genome database of C. sinensis is not yet available, we have successfully identified 62 protein spots using 2-DE-based mass analysis and EST database of C. sinensis. The proteins identified include detoxification enzymes, such as glutathione S-transferase and thioredoxin peroxidase, myoglobin and a number of cysteine proteases that are expressed abundantly. In order to identify potential targets for the diagnosis and therapy of clonorchiasis, we conducted immunoblot analysis of the ESPs proteome using the sera obtained from clonorchiasis patients and identified legumains and cysteine proteases as antigens present in the ESPs. Although the cysteine proteases were previously reported to elicit antigenicity, the legumains are found herein for the first time as a serological antigen of C. sinensis. To confirm these findings, we expressed recombinant legumain in Escherichia coli and verified that recombinant legumain also functions as a potent antigen against the sera of clonorchiasis patients. Our results illustrate the validity of immuno-proteomic approaches in the identification of serodiagnostic antigens in the parasites.</P>
PCR Diagnosis of Entamoeba histolytica Cysts in Stool Samples
Joung-Ho Moon,Shin-Hyeong Cho,Jae-Ran Yu,Won-Ja Lee,Hyeng-Il Cheun 대한기생충학열대의학회 2011 The Korean Journal of Parasitology Vol.49 No.3
Amebiasis is a protozoan disease caused by Entamoeba histolytica and a potential health threat in areas where sanitation and hygiene are inappropriate. Highly sensitive PCR methods for detection of E. histolytica in clinical and environmental samples are extremely useful to control amebiasis and to promote public health. The present study compared several primer sets for small subunit (SSU) rDNA and histone genes of E. histolytica cysts. A 246 bp of the SSU rDNA gene of pure cysts contained in phosphate-buffered saline (PBS) and in stool samples was successfully amplified by nested PCR, using the 1,147-246 bp primer set, of the primary PCR products which were pre-amplified using the 1,147 bp primer as the template. The detection limit of the nested PCR using the 1,147-246 primer set was 10 cysts in both groups (PBS and stool samples). The PCR to detect histone gene showed negative results. We propose that the nested PCR technique to detect SSU rDNA can be used as a highly sensitive genetic method to detect E. histolytica cysts in stool samples.