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Hee-Chul Park(박희철),Hongxuan Quan(전홍선),Yongjoon Kim(김용준),Hyeong-Cheol Yang(양형철) 대한치과재료학회 2017 대한치과재료학회지 Vol.44 No.4
This study aimed to examine the effect of phosphatidylserine-treated macrophages (THP-1) on the differentiation of human dental pulp cells. Phosphatidylserine, phosphatidylcholine, and cholesterol were mixed at a molar ratio of 2:1:1, and liposomes were produced via the vacuum evaporation method. Conditioned media (CM) were collected after treating macrophages, with liposomes for 6 and 24h, and were used for differentiating human dental pulp cells. To confirm the differentiation, we performed an evaluation of alkaline phosphatase (ALP) activity, reverse transcriptase polymerase chain reaction (RT-PCR) of dentin sialophosphoprotein (DSPP) and osteocalcin (OCN) gene expressions, and extracellular matrix mineralization assay via alizarin red S staining. There was a statistically significant increase in alkaline phosphatase activity with the conditioned medium containing 10% in the overall culture medium. RT-PCR assay revealed that conditioned media did not increase the mRNA expressions of DSPP and OCN genes. The degree of matrix mineralization was not affected by the conditioned medium. These results confirm a slight effect of the conditioned medium on the differentiation of human dental pulp cells. Future studies may adopt a method in which macrophages and human dental pulp cells are closely co-existed, to evaluate the effect of phosphatidylserine on the interaction between these cell types.
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