한국인 폐확산능 정상예측식의 임상적 유용성과 정확성

나승원 ( Seung Won Ra ),박태선 ( Tai Sun Park ),홍윤기 ( Yoon Ki Hong ),홍상범 ( Sang Bum Hong ),심태선 ( Tae Sun Shim ),임채만 ( Chae Man Lim ),이상도 ( Sang Do Lee ),고윤석 ( Youn Suck Koh ),김우성 ( Woo Sung Kim ),김동순 ( Dong 대한결핵 및 호흡기학회 2008 Tuberculosis and Respiratory Diseases Vol.64 No.2

연구배경: 폐확산능을 해석하는 데 필요한 정상예측식으로는 한국인을 대상으로 하여 박 등이 개발한 식(박 식)이 있으나 아직 외국 정상예측식을 많이 사용하고 있다. 이에 국내에서 많이 사용하는 외국 정상예측식인 Burrows 식과 박 식의 임상적 유용성과 정확성을 비교하고자 하였다. 방법: 1. 임상적 유용성 연구; 2006년 7월부터 12월까지 6개월간 폐확산능검사를 시행한 환자 중 두 식을 각각 정상예측식으로 적용하였을 때 폐확산능 해석이 다른 276명(대상군 A)을 대상으로 하였다. 대상군 A에게 두 식을 각각 적용하였을 때 폐확산능 해석과 임상적 판단과의 일치도를 비교하여 임상적 유용성을 평가하였다. 2. 간질성폐질환 진단의 정확성 비교; 2001년부터 2006년까지 폐조직검사를 시행하여 확진된 간질성폐질환군과 서울아산병원에서 모집한 정상군을 대상으로 하여 정상예측식으로 두 식을 각각 적용하였을 때 폐확산능 해석의 정확도를 비교하였고, 두 식이 차이가 나는지 맥니머의 카이스퀘어 검정을 하였다. 결과: 1. 임상적 판단과의 일치도 비교; 276명을 임상정보를 토대로 폐확산능을 예측하여 분류한 결과 정상 54명, 감소 220명, 불분명이 2명이었다. 예측식으로 박 식과 Burrows 식을 적용하였을 때 임상적 판단과 일치하는 환자는 각각 78%와 22%이었다(p<0.001). 2. 간질성폐질환 진단의 정확성 비교; 박 식은 민감도 90.1%, 특이도 100%이었고 Burrows 식은 민감도 64.2%, 특이도 100%로 민감도가 통계학적으로 유의하게 박 식이 높았다(p<0.001). 결론: 우리나라 정상예측식인 박 식이 외국 정상예측식인 Burrows 식을 정상예측식으로 적용하는 것보다 임상적 유용성이나 간질성폐질환 진단의 민감도에서 더 우월하였다. 향후 폐확산능검사의 정상예측식으로 박 식을 사용해야 할 것으로 사료된다. Background: Park et al. developed the Korean reference equation for the measurement of diffusing capacity in 1985. However, the equation has not been widely used in Korea and foreign reference equations have been popularly used. We intended to compare the clinical usefulness and the accuracy of the the Korean reference equation (Park`s equation) with that of the foreign equation (Burrows` equation) that is commonly used in Korea. Methods: 1. Evaluation of clinical usefulness; Among 1,584 patients who underwent diffusing capacity (DLCO) at the Asan Medical Center from July to December 2006, group A subjects included 276 patients who had different interpretations of DLCO in trials employing Burrows` equation and Park`s equation. Clinical assessment was decided by consensus of two respiratory physicians. In order to evaluate the clinical usefulness of Burrows` equation and Park`s equation, agreement of clinical assessment and DLCO interpretation were measured. 2. Evaluation of accuracy; Group B subjects were 81 patients with interstitial lung disease (ILD) and 39 normal subjects. The 81 ILD patients were diagnosed following a surgical lung biopsy. The accuracy of diagnosing ILD as well as sensitivity and specificity were evaluated according to the use of the reference equations (Burrows` equation and Park`s equation) for DLCO. Results: Agreement between clinical assessment and interpretation of DLCO was 22% for the use of Burrows` equation and 78% for the use of Park`s equation. The sensitivity and specificity of the Burrows` equation for diagnosing ILD were 64.2% and 100%. The sensitivity and specificity of the Park`s equation for diagnosing ILD were 90.1% and 100%. The sensitivity of the Park`s equation for diagnosing ILD was significantly higher than that of Burrows` equation (p<0.001). Conclusion: The Korean reference equation (Park`s equation) was more clinically useful and had higher sensitivity for diagnosing ILD than the foreign reference equation (Burrows` equation). (Tuberc Respir Dis 2008;64:80-86)

Analysis of monitoring Road-kill in Odaesan National Park

Sang Jin Lim,Ki Yoon Kim,Woo Chan Lim,Hyeong Woo Ji,Baek Soon Choi,Tae Il Kim,Hyo Nam Kim,Ji Hong Min,Hey Ri Kim,Eui Kyeong Kim,Yung Chul Park 강원대학교 산림과학연구소 2017 강원대학교 산림과학연구소 학술대회 Vol.2017 No.11

We surveyed mammalian roadkill at the National Route 6 (Jin-gogae to Songcheon, 18㎞) and a local road 446 (Odaesan National Park office to Sangwon-temple, 12㎞) in Odaesan National Park. Data of the surveys were analyzed by year, month, and species. Roadkills of 226 individuals were found at the survey areas. Of them, the road-killed 131 mammal individuals, 44 bird individuals, 40 reptile individual and 11 amphibian individuals were found during the survey period. High ratio of the road-kills was observed in mammals than the other animals. Among mammalian species, high ratio of the road-kills was observed in squirrels. There were road-kills of three endangered species, containing otters, yellow-throated marten and leopard cats. According to the analyses of annual road-kills, roadkills of 104 cases were occurred and highly frequent in 2014, following by roadkills of 81 cases in 2015, and then those of 41 cases in 2016. Most ases of the roadkills occurred in summer (June to September). The results will contribute to establishing management for reduction of roadkill in Odaesan National Park.

악성림프종 환자에서 복합화학요법으로 유발된 백혈구 감소증에 대한 인형 재조합 과립구 집락형성-촉진인자(rhG-CSF: Neutrogin)의 효과

박성규,전진우,기신영,유병우,김홍수,백승호,원종호,홍대식,박희숙 순천향의학연구소 1995 Journal of Soonchunhyang Medical Science Vol.1 No.1

Clinical effects of rhG-CSF were investigated in 17 patients receiving chemotherapy for non-Hodgkin's lymphoma. Patients were given G-CSF 2㎍/㎏/day after 2nd cycle chemotherapy for least 14 consecutive days, and clinical and laboratory results were compared to results of the first cycle. Treatment with G-CSF resulted in increase of mean WBC count(952.9±152.2㎜³vs 1882.4±350.4/㎜³) and neutrophil count(212.9±53.0/㎜³vs 841.5±238.0/㎜³), reduced the duration of leukocytopenic days(12.4±1.3 days vs 5.5±1.0 days, WBC count less than 4000/㎜³) and neutropenic days(4.5±0.9 days vs 2.3±0.7 days, neutrophil count less than 500/㎜³), and reduced the duration of neutropenic fever(5.8±1.1 days vs 0.9±0.5 days). The side effects of G-CSF were not so significant. These results demonstrated that G-CSF is safe and useful for the treatment of neutropenia induced by anticancer chemotherapy of non-Hodgkin's lymphoma and reducing the incidence of febrile periods and duration of antibiotic administration.

서울의 Penicillinase Producing Neisseria Gonorrhoeae 발생빈도(1996)

김재홍,황동규,전재홍,김윤석,김중환,김용준,이창균,임동진,김현수,조창근,김경문,박상훈,전우형,김희성,이호정,차명수,김갑형,김형석,김석우,황지환,박병순,권오상,이민수,송기훈,성소영,이인섭,부태성 대한화학요법학회 1999 대한화학요법학회지 Vol.17 No.2

Background : In recent years, gonorrhea has been panedemic and remains one of the most commom STDs in the world, especially in developing countries. Objective & Methods: For the detection of a more effective therapeutic regimen and assessing the prevalence of PPNG, we have been trying to study the patients who have visited the VD Clinic of Choong-Ku Public Health Center in Seoul since 1980 by means of the chromogenic cephalosporin method. Results: In 1996, 139 strains of N. gonorrhoeae were isolated, among which 53(39.0%) were PPNG. Conclusion: Our results suggests that after a peak of 74.3% in 1993, the prevalence of PPNG in Seoul is gradually declining.

Dexamethasone induces the expression of LRRK2 and α-synuclein, two genes that when mutated cause Parkinson`s disease in an autosomal dominant manner

( Ji Min Park ),( Dong Hwan Ho ),( Hye Jin Yun ),( Hye Jung Kim ),( Chan Hong Lee ),( Sung Woo Park ),( Young Hoon Kim ),( Il Hong Son ),( Won Gi Seol ) 생화학분자생물학회 2013 BMB Reports Vol.46 No.9

LRRK2 (leucine-rich repeat kinase 2) has been identified as a gene corresponding to PARK8, an autosomal-dominant gene for familial Parkinson`s disease (PD). LRRK2 pathogenicspecific mutants induce neurotoxicity and shorten neurites. To elucidate the mechanism underlying LRRK2 expression, we constructed the LRRK2-promoter-luciferase reporter and used it for promoter analysis. We found that the glucocorticoid receptor (GR) transactivated LRRK2 in a ligand-dependent manner. Using quantitative RT-PCR and Western analysis, we further showed that treatment with dexamethasone, a synthetic GR ligand, induced LRRK2 expression at both the transcriptional and translational levels, in dopaminergic MN9D cells. Dexamethasone treatment also increased expression of α-synuclein, another PD causative gene, and enhanced transactivation of the α-synuclein promoter-luciferase reporter. In addition, dexamethasone treatment to MN9D cells weakly induced cytotoxicity based on an LDH assay. Because glucocorticoid hormones are secreted in response to stress, our data suggest that stress might be a related factor in the pathogenesis of PD. [BMB Reports 2013; 46(9): 454-459]

Taxol 합성에 관한 연구 I :O-(tert-Butyldiphenylsilyl)-cis-5-methyl-4-hepten-6-yn-1-ol의 합성

박두천,주우홍,김형국,신동수 國立昌原大學校 基礎科學硏究所 1994 基礎科學硏究所論文集 Vol.6 No.-

O-(tert-Butyldiphenylsilyl)-cis-5-methyl-4-hepten-6-yn-1-ol, an intermediate in the synthesis of taxol, was efficiently synthesized from 3-methyl-2-penten-4-yn-1-ol by sequential alcohol/bromide interchange [NBS, PPh₃, CH₂Cl₂-30.℃, 82%], displacement with the lithium salt of tert-butylacetate [LDA, THF, -78 to 23℃, 84%], LAH reduction, and silylation [tBuPhSiCl, imidazole, CH₂Cl₂, 45℃, 88%]

초·중등학교 과학탐구활동 및 체험학습 프로그램의 운용실태 조사

박윤배,정원우,이광필,임석훈,박홍배 慶北大學校 科學敎育硏究所 2004 科學敎育硏究誌 Vol.28 No.-

The purpose of this study is to survey on the program of inquiry activity and field excursion in science education of elementary and secondary schools. A total of 1,218 elementary students, 437 secondary students, 1,070 parents, and 120 teachers from Daegu area were responded to our survey. Questionnaires for students were about interest on science and science instruction, career choice, and science inquiry experience: for parents were about need of science and technology, children's career choice, and science base environment: for teachers were science base environment and effort. Parents were well recognized the importance of science. Students also have generally expressed the interest about science and science instruction, but the higher their grades, the less the interest level because of difficulty of science. About 15% of students were planned to search their career in science and technology area. To broaden the base of science environment, teachers and parents said that government had the greatest affect. Students responses showed that they did not satisfied with their out-of-school inquiry activities.

카드뮴폭로에 대한 생물학적 지표 : Competitive ELISA에 의한 Metallothionein 정량 Assay of Metallothionein by Competitive ELiSA

박정덕,이우석,공윤,홍연표,장임원 大韓産業醫學會 1996 대한직업환경의학회지 Vol.8 No.2

Metallothionein (MT) is a cadmium binding protein that played major roles in protective mechanism for cadmium toxicity. In the present study, competitive ELISA was established to assay the MT expression utilizing monospecific antibody which was generated against MT-I. A total of 10 Sprague-Dawley rats was injected with CdCl₂ for two weeks to induce MT. The cytosolic fraction of rat liver was obtained by differential centrifugation. Two major MT isozymes (MT-I & MT-II) at ca. 10 kDa were purified by Sephadex G-75 gel filtration followed by DEAE-Trisacryl-M anion exchange column chromatography, respectively. Two rabbits were immunized 4 times consecutively with the conjugate of purified MT-I. The sera were collected by heart puncture. When immunoblot was carried out, the immunized rabbit sera (anti-MT-I) exhibited specific immunoreactive band at MT-I while showed any cross reactions neither with MT-II nor with other cytosolic proteins. By chequerboard titration using the monospecific antibody, the competitive ELISA was established. The dose-dependent relationship was observed between anti-MT-I anti-body and the amount of MT in biological samples (r²=0.9980). These results suggested strongly that competitive ELISA is a simple, rapid and reproducible method for screening cadmium exposure.

全南地方의 水稻白葉枯病菌 菌型과 그의 地域別 및 品種別 分布

朴淳直,金基淸,禹洪斗,崔容壽 全南大學校 農漁村開發硏究所 1986 農業科學技術硏究 Vol.21 No.-

This was carried out to identify the pathotypes of Xanthomonas campestris pv. oryzae collected from regional exhibition paddys and others in Jeonnam province, and to investigate the sub-regional and varietal distributions of them in 1982. Collected isolates were identified as pathotypes Ⅰ, Ⅱ, Ⅲ and 2 unknown groups with Korean differentials; Ⅰ, Ⅱ, Ⅲ, Ⅳ,Ⅴ and 4 unknown with Japanese; Ⅰ, Ⅱ and 2 unknown with IPPI's, respectively. The fact that unknown groups were isolated suggest that some differentiation is occurring among the previous pathotypes. Using Japanese differentials, pathotype was isolated from l1 sub-regions, Ⅱ and gⅢfrom 4, g from I, g from 3, and unknown 4 groups from 8, respectively. However, there was no sub-region where all 5 pathotypes were detected. Pathotypes with Japanese differentials was isolated from 6 varieties, Iri 346 and 347, Milyang 23 and 30, Jinjubyo and Nakdongbyo: pathotype Ⅱ from 6 varieties, Suvreon 294, Irl 342 and 347, Jinjubyo and Akihikari; pathotype Ⅲ from 5 varieties, Suweon 294, Iri 346, Milyang 30 and Nongrim 41; pathotype Ⅳ from 1 variety, Jinjubyo; pathotype V from 4 varieties, Suweon 294, Iri 347, Milyang 23 and Jinjubyo, respectively.

中學校 科學科의 實驗實習敎材의 開發硏究Ⅱ

朴泳喆,韓麟傳,李春雨,權寧駿,朴弘緖 공주대학교 사범대학 과학교육연구소 1982 과학교육연구 Vol.14 No.1

In this study a few laboratory teaching materials have been developed to be available for the effective performance of experiments and practices- a heart of inquiry learning-as a continuance of 'A study on the Development of Laboratory Teaching Materials for Middle School Science Course I' The present condition of science education has been investigated by means of questionaires, interviews and literature, the results of the present condition and problems in junior high schools are as follows: 1. The hours for science classes are considerably little for the teaching contents of science textbooks. 2. The level of teaching is beyond the student's capability in terms of their experience and intellectual development. 3. Science teachers are overburdened with much tasks, and laboratories and laboratory facilities are too insufficient and furthermore teaching is mainly conducted on the lecture method as a preperation for various entrance exams. 4. Teachers' eagerness and desirable attitude for teaching are required. In this study, materials for every branch of science have been developed to be available for the effective performance of experiments and practices through inquiries. The materials developed in this study are as follows: 1. In physics, measure apparatus of extremely small masses, length, and very little time intervals are developed. 2. In chemistry, new methods for gas generating apparatus and materials. are developed. 3. In biology, the size of quadrate and the method to decide the time in facilitating for the purpose of survey of botany community are introduced. The results of the investigation of natural enemy on Hyphantria cunea, and the study on collecting, breeding of Planaria are also shown. 4. In earth science, the laboratory teaching materials are developed for observing continuous spectrum and line spectrum. Putting the above results of study together, the suggestions are made to the effect as follows: 1. The quantity of learning should be reduced and the levels lowered in the new textbooks. 2. The development and distribution of various teaching materials(film, T,P., cassettes film, supplementary, textbook, program teaching material, individual learning material) is required. 3. The experimental instruments should be timely manufactured and distributed on the basis of effective administrative support to ensure their solidity and accuracy. 4. The concrete design to eliminate the teacher's burden should be contrived and the system for senion teachers in charge of science teaching in secondary schools, should be improved. 5. Teacher's research activities should be encouraged through various research institutes(Institute of science education , graduate school of education and the other research institutes.) 6. The disposition of laboratory assistant should be considered. 7. More positive supports from other societies relevant with education are much expected to be made.