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      • 고분보존용 항온ㆍ항습 System의 동절기 운전특성

        이금배,전용두,고석보,김홍기 公州大學校 工學硏究院 生産技術硏究所 2003 論文集 Vol.11 No.-

        본 연구는 여러 가지 문화재 보존영역 중 개방된 고분의 장기보존을 위한 최적 공기 조화 시스템의 개발을 목표로 하여 수행되었다. 고분의 장기보존을 위해서는 고분 내 공기조성, 온ㆍ습도 및 기류환경을 모니터하여 고분내 자연 실내환경의 기초자료를 확보한 수 고분의 주 구성요소인 석재 및 전재의 보조측면에서 문제점을 검토하여 최적 유지환경조건을 정의하고, 이상의 요구조건을 실현할 수 있는 공조시스템의 적용이 필요하다. 본 논문은 이 중 모형고분에 적용된 공조시스템의 동계운전성능에 대하여 소개한다.

      • 거주자우선주차제의 관리시스템 개발

        금기정,이근희,이홍범,김명수 明知大學校 産業技術硏究所 2000 産業技術硏究所論文集 Vol.19 No.-

        Increase of incoming level and improvement of welfare caused by rapid economic growth make a sudden increase of car-owners. Such according to vehicle increases, arose many social Problems centering around metropolis. This in Problems, the most parking problem mainly raise in relation between unequality of parking demand and supply. Though it is important that increase the quantity of parking zone in metropolis that develope rapidly motorization it is more meaningful that manage systematically at limited parking area in urban. With these problems, in developing The Parking Management Assistant System improve the problem that The Residential Parking Permit Program(RPPP)that carrying out in Seoul. The Parking & Geographic Management System(P&GMS) is constituted to develope systematically Parking Use Information that intend to use the parking area efficiently. Consequently improve indicated problem which existing system and improving of use efficiency. Furthermore The Parking Management System is expected to help the improvement of transportation information through connection to ITS, such as ATIS, that recognized as central function in The Parking Permit Zone Project which is executed in Seoul now.

      • 동절기 신관리 석실고분 내부의 온·습도 분포특성

        전용두,이금배,김홍기,고석보 공주대학교 문화재보존과학연구소 2004 문화재과학기술 Vol.3 No.1

        개방된 고분의 장기보존은 조상의 얼이 담긴 문화재를 길이 후손에 물려주어야 하는 우리들의 시대적 사명이라 할 수 있다. 그런데 고분 내부의 공기조화를 필요로 하는 경우 설비측면에서 고분내부의 공기조화 문제를 검토한 사례는 그 동안 많지 않았다. 특히 고분 내부환경에 관한 온·습도 자료는 매우 제한적으로 알려져 있는 상태이다. 본 연구는 고분의 공기조화와 관련하여 부하산정 등의 기초자료로 활용하기 위한 고분내부의 온·습도자료를 원격측정 기술을 적용하여 획득한 결과를 소개하며, 이를 통하여 고분내부의 실내환경 조건에 대한 일반의 이해를 돕고, 더 나아가 공조기 설치에 필요한 기초자료를 제공하고자 수행하였다. 측정 연구대상은 충남 공주시 신관동에 소재한 신관리석실고분(충청남도 기념물 제 7호)으로서 백제후기의 횡혈식 석실고분으로서 현실의 크기는 폭 1.25m, 깊이 2.73m 그리고 높이 1.50m이며, 현실입구는 개방된 상태에서 실내 5채널 및 실외공기에 대하여 각각 온도와 습도를 측정하였으며, 본 본문에서는 동절기(12월부터 다음 해 3월까지)의 자료를 소개한다. In recent years the importance of the preservation of cultural artefacts like ancient tombs has been widely accepted domestically and internationally as the quality of life improves. However not much attention has been paid for the facilities and systems to preserve those artefacts. Even the general understanding of the preservatory environment of the underground space as tombs is poor. In the present study, the temperature and relative humidity inside a selected artefact, Shinkwan-ri tomb, are monitored to improve the understanding of the indoor thermal environment, which may possibly be used when one consider air-conditioning for preservation. Total of 12 channel data are collected by using remote data acquisition system based on the cdma(Code Division Multiple Access) technology. Three-month-data representing winter season is reported with discussions.

      • Paraquat 투여 백서에서 Cyclophosphamide와 Dexamethasone의 치료 효과

        신정철,신양수,유병전,임현성,정태흥,박찬국,오금탁,이병래 朝鮮大學校 附設 醫學硏究所 1991 The Medical Journal of Chosun University Vol.16 No.1

        Paraquat (N,N'-dimethy 1-4,4'bipyrydinium : Gramoxone^(R), a widely used herbicide, is extremely toxic to all green plants and many eukaryotic organisms. In human, paraquat intoxication leads to multiple organ failure, it preferentially damages the lung, kidney and liver and may result in death. The participation of superoxide in initiating tissue damage by administration of paraquat is well known in which one electron reduction of paraquat leads to the formation of radical species which react with molecular oxygen to give superoxide. This process initiates immunological changes with the activation of neutrophilic leukocytes, which is related to further production of superoxide. In this experiment, therapeutic effects of cyclophosphamide and dexamethasone on blood cell count, malondialdehyde as a indicator of lipid peroxidation, and antioxidant enzymes levels in the lung, liver and kidney of paraquat intoxicated rats were inbestigated. The WBC count were significantly decreased by combined treatment of cyclophosphamide and dexamethasone in paraquat intoxicated rats at 3 and 5 days but RBC count were not changed. The contents of malondialdehyde were significantly decreased in the lung and Kidney by combined treatment at 10 days after paraquat intoxication. The catalase acitvities were markedly increased in the lung and liver of rats at 10 days after paraquat intoxication, and superoxide dismutase activities showed minimal changes in the lung, liver and kidney of rats by combined treatment. These results suggest that paraquat induced tissue damage in rats can be redeced by combined administration of cyclophosphamide and dexamethasone.

      • SCIESCOPUS

        Biodiversity and physiological characteristics of Antarctic and Arctic lichens-associated bacteria.

        Lee, Yung Mi,Kim, Eun Hye,Lee, Hong Kum,Hong, Soon Gyu Springer 2014 World journal of microbiology biotechnology Vol.30 No.10

        <P>The diversity and physiological characteristics of culturable bacteria associated with lichens from different habitats of the Arctic and Antarctica were investigated. The 68 retrieved isolates could be grouped on the basis of their 16S rRNA gene sequences into 26 phylotypes affiliated with the phyla Actinobacteria, Bacteroidetes, Deinococcus-Thermus, and Firmicutes and with the classes Alphaproteobacteria, Betaproteobacteria, and Gammaproteobacteria. Isolates belonging to the Alphaproteobacteria were the most abundant, followed by those belonging to Actinobacteria, Betaproteobacteria, Gammaproteobacteria, Bacteroidetes, Firmicutes, and Deinococcus-Thermus. Phylogenetic analysis showed that approximately 21 % of the total isolates represented a potentially novel species or genus (97 % sequence similarity). Strains belonging to the genera Sphingomonas, Frondihabitans, Hymenobacter, and Burkholderia were recovered from lichen samples from both geographic locations, implying common and important bacterial functions within lichens. Extracellular protease activities were detected in six isolates, affiliated with Burkholderia, Frondihabitans, Hymenobacter, Pseudomonas, and Rhodanobacter. Extracellular lipase activities were detected in 37 isolates of the genera Burkholderia, Deinococcus, Frondihabitans, Pseudomonas, Rhodanobacter, Sphingomonas, and Subtercola. This is the first report on the culturable bacterial diversity present within lichens from Arctic and Antarctica and the isolates described herein are valuable resources to decode the functional and ecological roles of bacteria within lichens. In addition, the low similarity (97 %) of the recovered isolates to known species and their production of cold-active enzymes together suggest that lichens are noteworthy sources of novel bacterial strains for use in biotechnological applications.</P>

      • SCIESCOPUSKCI등재

        Analysis of Expressed Sequence Tags from the Red Alga Griffithsia okiensis

        Lee, Hyoung-Seok,Lee, Hong-Kum,An, Gyn-Heung,Lee, Yoo-Kyung The Microbiological Society of Korea 2007 The journal of microbiology Vol.45 No.6

        Red algae are distributed globally, and the group contains several commercially important species. Griffithsia okiensis is one of the most extensively studied red algal species. In this study, we conducted expressed sequence tag (ESTs) analysis and synonymous codon usage analysis using cultured G. okiensis samples. A total of 1,104 cDNA clones were sequenced using a cDNA library made from samples collected from Dolsan Island, on the southern coast of Korea. The clustering analysis of these sequences allowed for the identification of 1,048 unigene clusters consisting of 36 consensus and 1,012 singleton sequences. BLASTX searches generated 532 significant hits (E-value <$10^{-4}$) and via further Gene Ontology analysis, we constructed a functional classification of 434 unigenes. Our codon usage analysis showed that unigene clusters with more than three ESTs had higher GC contents (76.5%) at the third position of the codons than the singletons. Also, the majority of the optimal codons of G. okiensis and Chondrus crispus belonging to Bangiophycidae were G-ending, whereas those of Porphyra yezoensis belonging to Florideophycidae were G-ending. An orthologous gene search for the P. yezoensis EST database resulted in the identification of 39 unigenes commonly expressed in two rhodophytes, which have putative functions for structural proteins, protein degradation, signal transduction, stress response, and physiological processes. Although experiments have been conducted on a limited scale, this study provides a material basis for the development of microarrays useful for gene expression studies, as well as useful information for the comparative genomic analysis of red algae.

      • SCIESCOPUSKCI등재

        Bacterial Diversity of Culturable Isolates from Seawater and a Marine Coral, Plexauridae sp., near Mun-Sum, Cheju-Island

        Lee, Jung-Hyun,Shin, Hyun-Hee,Lee, Deuk-Soo,Kwon, Kae-Kyung,Kim, Sang-Jin,Lee, Hong-Kum The Microbiological Society of Korea 1999 The journal of microbiology Vol.37 No.4

        Fifty-eight strains showing different colony morphological characteristics on various media were isolated from marine coral (Plexauridae sp.) and ambient seawater near Mun-Sum, Cheju-Island in 1998. Bacterial diversity was studies by phylogenetic analysis of the partial 16S rRNA gene sequences. All isolates representing the bacterial domain included affiliates of the high G+C (59%) and los G+C (3%) subdivision of Gram positive bacteria, and the alpha (33%) and gamma (5%) subdivision of the Proteobacteria. The 16S rDNA sequence similarity of the isolates was in the 88.3 to 100% range (average, 95.6%) to reported sequence data. In the comparison of the isolates from marine coarl and ambient seawater, more diverse groups belonging to ${\alpha}$-Proteobacteria were preferentially obtained from seawater.

      • SCIESCOPUSKCI등재

        Optimal Protocol for Enumeration of Attached Bacteria on Glass Slides

        Lee, Hyun-Sang,Kwon, Kae-Kyoung,Lee, Jong-Ho,Lee, Hong-Kum The Microbiological Society of Korea 1999 The journal of microbiology Vol.37 No.4

        In examining bacterial growth on glass surfaces immersed in sea water, we found serious differences between enumeration methods. Therefore, we compared various methods and found sonication and direct count methods were superior to other methods. Since the direct count method was not suitable for long-term investigation, we chose the sonication method and confirmed that sonication periods 8 times for 30 seconds was optimal for the detachment of bacteria from glass surfaces.

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