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RISS 인기검색어
- 검색키워드
- 저자 : Hiroki Iwata (검색결과 3 건)
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Hiroki Iwata,Yuji Okada,Kiyoshi Ohishi,Yuki Yokokura 전력전자학회 2019 ICPE(ISPE)논문집 Vol.2019 No.5
This paper proposes a new compensation method for estimating temperature using a voltage disturbance observer. In conventinal resistance estimation method is weekness to other parameter variations in SPMSM, because it is for estimating potision control system. In addition, offline compensate method is hard, because rotor flux and inductance are changing depending on temperature. Hence, we proposed a method to estimate the temperature based on the motor model without the influence of back erectromotive force. However, problems such as voltage disturbances arise, because the system didnot consider the inductance variation depending on temperature. This paper proposes a new method to compensate these disturbances and confirm its effectiveness by experiments.
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Teraoka, Hiroki,Ito, Shino,Ikeda, Haruki,Kubota, Akira,Abou Elmagd, M M,Kitazawa, Takio,Kim, Eun-Young,Iwata, Hisato,Endoh, Daiji American Chemical Society 2012 Environmental science & technology Vol.46 No.1
<P>To assess possible impacts of environmental pollutants on gene expression profiles in a variety of organisms, we developed a novel differential display system with primer sets that are common in seven vertebrate species, based on degenerate oligonucleotide-primed PCR (DOP-PCR). An 8-mer inverse repeat motif was found in most transcripts from the seven vertebrates including fish to primates with detailed transcriptome information; more than 10,000 motifs were recognized in common in the transcripts of the seven species. Among them, we selected 275 common motifs that cover about 40-70% of transcripts throughout these species, and designed 275 DOP-PCR primers that were common to seven vertebrate species (common DOP-PCR primers). To detect genes responsive to 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) in developing embryos, differential display with common DOP-PCR primers was applied to embryonic liver of two avian species, the chicken (Gallus gallus) and the common cormorant (Phalacrocorax carbo), which were exposed in ovo to TCDD. The cDNA bands that showed differences between the control and TCDD-treated groups were sequenced and the mRNA expression levels were confirmed by real-time RT-PCR. This approach succeeded in isolating novel dioxin-responsive genes that include 10 coding genes in the chicken, and 1 coding gene and 1 unknown transcript in the cormorant, together with cytochrome P450 1As that have already been well established as dioxin markers. These results highlighted the usefulness of systematically designed novel differential display systems to search genes responsive to chemicals in vertebrates, including wild species, for which transcriptome information is not available.</P>
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Dau, Pham Thi,Sakai, Hiroki,Hirano, Masashi,Ishibashi, Hiroshi,Tanaka, Yuki,Kameda, Kenji,Fujino, Takahiro,Kim, Eun-Young,Iwata, Hisato Academic Press 2013 Toxicological sciences Vol.131 No.1
<P>The constitutive androstane receptor (CAR) not only displays a high basal transcriptional activity but also acts as a ligand-dependent transcriptional factor. It is known that CAR exhibits different ligand profiles across species. However, the mechanisms underlying CAR activation by chemicals and the species-specific responses are not fully understood. The objectives of this study are to establish a high-throughput tool to screen CAR ligands and to clarify how CAR proteins from the Baikal seal (bsCAR) and the mouse (mCAR) interact with chemicals and steroid receptor coactivator 1 (SRC1). We developed the surface plasmon resonance (SPR) system to assess quantitatively the interaction of CAR with potential ligands and SRC1. The ligand-binding domain (LBD) of bsCAR and mCAR was synthesized in a wheat germ cell-free system. The purified CAR LBD was then immobilized on the sensor chip for the SPR assay, and the kinetics of direct interaction of CARs with ligand candidates was measured. Androstanol and androstenol, estrone, 17β-estradiol, TCPOBOP, and CITCO showed compound-specific but similar affinities for both CARs. The CAR-SRC1 interaction was ligand dependent but exhibited a different ligand profile between the seal and the mouse. The results of SRC1 interaction assay accounted for those of our previous in vitro CAR-mediated transactivation assay. In silico analyses also supported the results of CAR-SRC1 interaction; there is little structural difference in the ligand-binding pocket of bsCAR and mCAR, but there is a distinct discrimination in the helix 11 and 12 of these receptors, suggesting that the interaction of ligand-bound CAR and SRC1 is critical for determining species-specific and ligand-dependent transactivation over the basal activity. The SPR assays demonstrated a potential as a high-throughput screening tool of CAR ligands.</P>
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