http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
- 中文 을 입력하시려면 zhongwen을 입력하시고 space를누르시면됩니다.
- 北京 을 입력하시려면 beijing을 입력하시고 space를 누르시면 됩니다.
RISS 인기검색어
- 검색키워드
- 저자 : Haicong Liu (검색결과 1 건)
- 무료
- 기관 내 무료
- 유료
-
Transcriptome Analysis of the Cytokinin Response in Medicago truncatula
Zhixiang Zhou,Haicong Liu,Cuina Ma,Yuehui Chao,Liebao Han 한국식물학회 2020 Journal of Plant Biology Vol.63 No.3
As an important legume plant, Medicago truncatula is a preeminent model for the study of the processes of nitrogen fxation, symbiosis, and legume genomics. The regulatory mechanism of the cytokinin response has been studied in many plants, such as rice, Arabidopsis, tomato, and barley, but information about regulatory pathways and genes involved in the cytokinin response in Medicago truncatula is notably limited. In this study, to better understand the cytokinin response in Medicago truncatula, transcriptome analysis of seedlings grown with 6-benzylaminopurine or lovastatin was performed using RNASeq. In this study, 3627 and 3093 transcripts were diferentially expressed in cytokinin-induced/control (Cyto/Ctrl) and cytokinin-inhibited/control (Inh/Ctrl) groups, respectively, and diferentially expressed genes were tested by quantitative real-time PCR (qRT-PCR). Analysis of the cytokinin response in Medicago truncatula revealed a large number of transcripts involved in signal transduction, metabolic process, secondary metabolite biosynthesis, transport and catabolism, growth and development, defense mechanisms, and transcription. There were 43 transcription factor families, including 1845 transcription factor (TF) genes with 2147 TF transcripts, as detected by RNA-Seq. Additionally, 216 TF genes with 220 transcripts were diferentially expressed in Cyto/Ctrl, and 185 TF genes with 189 transcripts were in Inh/Ctrl. A total of 289 and 260 DETs involved in biosynthesis, metabolism, and transduction of plant hormones were identifed in the Cyto/Ctrl and Inh/ Ctrl groups, respectively. Furthermore, 15 transcripts, including A-ARR, IPT, and CKX, were demonstrated to play roles in cytokinin regulation or signal transduction. These fndings were associated with the cytokinin response in other plants. The resulting data provide the frst cytokinin transcriptome analysis in Medicago truncatula. Further analysis and identifcation of cytokinin-regulated transcripts or signal transduction transcripts may help to elucidate the regulatory mechanisms governing the cytokinin response in Medicago truncatula.
연관 검색어 추천
이 검색어로 많이 본 자료
활용도 높은 자료
