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      • KCI등재

        Plant mediated green synthesis and antibacterial activity of silver nanoparticles using Crataegus douglasii fruit extract

        Mansour Ghaffari-Moghaddam,Robabeh Hadi-Dabanlou 한국공업화학회 2014 Journal of Industrial and Engineering Chemistry Vol.20 No.2

        In this study, silver nanoparticles were synthesized using the Crataegus douglasii fruit extract as areducing agent. The reaction process was monitored by UV–vis spectroscopy. Further characterizationwas carried out using scanning electron microscopy (SEM). To optimize the biosynthesis of silvernanoparticles, the effect of process variables such as extract concentrations, mixing ratio of the reactants,time and pH were also investigated. The SEM images showed silver nanoparticles with 29.28 nm size andnearly spherical shape at 24 h interaction time. The antibacterial activity of the synthesized silvernanoparticles was confirmed against Staphylococcus aureus and Escherichia coli.

      • KCI등재

        Green synthesis of silver nanoparticles using plant extracts

        Mansour Ghaffari-Moghaddam,Robabeh Hadi-Dabanlou,Mostafa Khajeh,Mansoureh Rakhshanipour,Kamyar Shameli 한국화학공학회 2014 Korean Journal of Chemical Engineering Vol.31 No.4

        The strategy for design of new nanometals was developed due to their wide applications in many fields. One of the most important nanometals is silver nanoparticles (AgNPs) because of their extensive applications in biotechnologyand biomedical fields. AgNPs were usually synthesized by using chemical and physical methods. In thechemical methods, various toxic chemicals are used, which are harmful to the health of living organisms. Therefore,the AgNPs were synthesized by using biological methods based on green chemistry for reducing the toxic chemicals. There are various resources for green synthesis of AgNPs, such as bacteria, fungi, enzyme and plant extracts. The greensynthesis of AgNPs involves three main steps: the selection of the solvent medium, the selection of environmentallyreducing agents, and the selection of non-toxic substances for the stability of AgNPs. The biosynthesis of AgNPs usingplant extracts is more favorable than other biological methods because of removing the elaborate process of maintainingcell cultures. It can be also suitably scaled up for large scale production of AgNPs. This review focuses on green synthesisof AgNPs using various plant extracts.

      • KCI등재

        Human parvovirus B19 and parvovirus 4 among Iranian patients with hemophilia

        Davod Javanmard,Masood Ziaee,Hadi Ghaffari,Mohammad Hasan Namaei,Ahmad Tavakoli,Hamidreza Mollaei,Mohsen Moghoofei,Helya Sadat Mortazavi,Seyed Hamidreza Monavari 대한혈액학회 2017 Blood Research Vol.51 No.4

        Background: Human parvovirus B19 (B19V) is one of the smallest DNA viruses and shows great resist-ance to most disinfectants. Therefore, it is one of the common contaminant pathogens present in blood and plasma products. Parvovirus 4 (PARV4) is a newly identified parvovi-rus, which is also prevalent in parenteral transmission. In this study, we aimed to evaluate the prevalence of B19V and PARV4 DNA among patients with hemophilia in Birjand County in eastern Iran. Methods: This was a cross-sectional epidemiological study comprising nearly all people with hemo-philia in this region. Whole blood samples were taken after patient registration and sent for plasma isolation. After nucleic acid extraction, B19V was detected with real-time poly-merase chain reaction, PARV4 DNA was then detected using sensitive semi-nested PCR. Results: In total, there were 86 patients with hemophilia, with mean age 28.5±1.5 years. Of these, 90.7% were men and 9.3% women; 84.9% had hemophilia A and 7.0% had hemophilia B. We found 11 patients (12.8%) were positive for B19V DNA and 8 were positive (9.3%) for PARV4 DNA. The prevalence of B19V was higher in middle-aged groups rather than younger people, whereas PARV4 infection was more common in younger patients (P<0.05). Conclusion: There was a high prevalence of B19V and PARV4 infection in this high-risk group of pa-tients with hemophilia. Due to the clinical significance of the B19 virus, imposing more precautionary measures for serum and blood products is recommended.

      • KCI등재

        Human parvovirus B19 and parvovirus 4 among Iranian patients with hemophilia

        Davod Javanmard,Masood Ziaee,Hadi Ghaffari,Mohammad Hasan Namaei,Ahmad Tavakoli,Hamidreza Mollaei,Mohsen Moghoofei,Helya Sadat Mortazavi,Seyed Hamidreza Monavari 대한혈액학회 2017 Blood Research Vol.52 No.4

        Background: Human parvovirus B19 (B19V) is one of the smallest DNA viruses and shows great resist-ance to most disinfectants. Therefore, it is one of the common contaminant pathogens present in blood and plasma products. Parvovirus 4 (PARV4) is a newly identified parvovi-rus, which is also prevalent in parenteral transmission. In this study, we aimed to evaluate the prevalence of B19V and PARV4 DNA among patients with hemophilia in Birjand County in eastern Iran. Methods: This was a cross-sectional epidemiological study comprising nearly all people with hemo-philia in this region. Whole blood samples were taken after patient registration and sent for plasma isolation. After nucleic acid extraction, B19V was detected with real-time poly-merase chain reaction, PARV4 DNA was then detected using sensitive semi-nested PCR. Results: In total, there were 86 patients with hemophilia, with mean age 28.5±1.5 years. Of these, 90.7% were men and 9.3% women; 84.9% had hemophilia A and 7.0% had hemophilia B. We found 11 patients (12.8%) were positive for B19V DNA and 8 were positive (9.3%) for PARV4 DNA. The prevalence of B19V was higher in middle-aged groups rather than younger people, whereas PARV4 infection was more common in younger patients (P<0.05). Conclusion: There was a high prevalence of B19V and PARV4 infection in this high-risk group of pa-tients with hemophilia. Due to the clinical significance of the B19 virus, imposing more precautionary measures for serum and blood products is recommended.

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