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      • KCI등재

        Complete Genome Sequencing of Bacillus velezensis WRN014, and Comparison with Genome Sequences of other Bacillus velezensis Strains

        ( Junru Wang ),( Juyuan Xing ),( Jiangkun Lu ),( Yingjiao Sun ),( Juanjuan Zhao ),( Shaohua Miao ),( Qin Xiong ),( Yonggang Zhang ),( Guishan Zhang ) 한국미생물생명공학회(구 한국산업미생물학회) 2019 Journal of microbiology and biotechnology Vol.29 No.5

        Bacillus velezensis strain WRN014 was isolated from banana fields in Hainan, China. Bacillus velezensis is an important member of the plant growth-promoting rhizobacteria (PGPR) which can enhance plant growth and control soil-borne disease. The complete genome of Bacillus velezensis WRN014 was sequenced by combining Illumina Hiseq 2500 system and Pacific Biosciences SMRT high-throughput sequencing technologies. Then, the genome of Bacillus velezensis WRN014, together with 45 other completed genome sequences of the Bacillus velezensis strains, were comparatively studied. The genome of Bacillus velezensis WRN014 was 4,063,541bp in length and contained 4,062 coding sequences, 9 genomic islands and 13 gene clusters. The results of comparative genomic analysis provide evidence that (i) The 46 Bacillus velezensis strains formed 2 obviously closely related clades in phylogenetic trees. (ii) The pangenome in this study is open and is increasing with the addition of new sequenced genomes. (iii) Analysis of single nucleotide polymorphisms (SNPs) revealed local diversification of the 46 Bacillus velezensis genomes. Surprisingly, SNPs were not evenly distributed throughout the whole genome. (iv) Analysis of gene clusters revealed that rich gene clusters spread over Bacillus velezensis strains and some gene clusters are conserved in different strains. This study reveals that the strain WRN014 and other Bacillus velezensis strains have potential to be used as PGPR and biopesticide.

      • KCI등재

        Multistep Controllability Synthesis and Growth Mechanism of ZnO Nanopagoda for Schottky Diode Device

        Yang Liu,Guishan Liu,Yongbing Wang,Wenyuan Gao,Hongshun Hao,Bopu Huang 성균관대학교(자연과학캠퍼스) 성균나노과학기술원 2016 NANO Vol.11 No.2

        Ordered ZnO arrays with a peculiar nanostructure were synthesized by a multistep synthesis process. The first step was the preparation of ZnO seed to induce the formation of ZnO array via potentiostatic electrodeposition method using a typical three electrode set-up. The second step was fabricating ZnO array along seed by Chemical Bath Deposition. Structural analysis of ZnO was carried out with X-ray diffraction (XRD), which showed a hexagonal wurtzite structure, and the selected area electron diffraction (SAED) patterns indicated that nanocrystalline is a part of monocrystal. The scanning electron microscope (SEM) and transmission electron microscope (TEM) were used to study the microstructure, and showed a pagoda-like microstructure with a tiny top and large bottom, which had an average top diameter of exceeding 800 nm at seeddepositing time of 60 s, and then growth mechanisms are subsequently given a further explanation, viewed from kinetics and thermodynamics. In addition, the current–voltage curves of schottky diode devices with ZnO nanopagoda arrays revealed that ZnO films arrays along grown ZnO seed had a higher reverse saturation current than ZnO films grown without seed, which are 1.48 x 10-6 A and 1.32 x 10-8 A, respectively. The minimum turn-on voltage of the diode with ZnO seed deposited 60 s is 0.18 V, without seed is 0.52 V.

      • KCI등재

        Low-Cost Flexible Strain Sensor Based on Thick CVD Graphene

        Bailiang Chen,Ying Liu,Guishan Wang,Xianzhe Cheng,Guanjun Liu,Jing Qiu,Kehong Lv 성균관대학교(자연과학캠퍼스) 성균나노과학기술원 2018 NANO Vol.13 No.11

        Flexible strain sensors, as the core member of the family of smart electronic devices, along with reasonable sensing range and sensitivity plus low cost, have rose a huge consumer market and also immense interests in fundamental studies and technological applications, especially in the field of biomimetic robots movement detection and human health condition monitoring. In this paper, we propose a new flexible strain sensor based on thick CVD graphene film and its low-cost fabrication strategy by using the commercial adhesive tape as flexible substrate. The tensile tests in a strain range of ~30% were implemented, and a gage factor of 30 was achieved under high strain condition. The optical microscopic observation with different strains showed the evolution of cracks in graphene film. Together with commonly used platelet overlap theory and percolation network theory for sensor resistance modeling, we established an overlap destructive resistance model to analyze the sensing mechanism of our devices, which fitted the experimental data very well. The finding of difference of fitting parameters in small and large strain ranges revealed the multiple stage feature of graphene crack evolution. The resistance fallback phenomenon due to the viscoelasticity of flexible substrate was analyzed. Our flexible strain sensor with low cost and simple fabrication process exhibits great potential for commercial applications.

      • KCI등재

        Effect of sintering atmosphere on the structure and dielectric properties of BSIT ceramics

        Yue Liu,Kunzhi Luan,Jiahao Xu,Zhen Wang,Yike He,Guishan Liu 한양대학교 청정에너지연구소 2023 Journal of Ceramic Processing Research Vol.24 No.4

        In this paper, the effects of O2 and N2 sintering atmospheres on the physical phase and microstructure of BSIT ceramics wereinvestigated by using In3+-doped BST ceramics as the research object. And then the dielectric properties of the ceramics inthe frequency range of 20Hz-300000 Hz were investigated by constructing ceramic capacitors. The results show that theceramic sintered in N2 atmosphere has a denser morphology and a higher dielectric constant of 862 compared with the ceramicsintered in O2 atmosphere, and the dielectric loss is reduced by about 39% in the high frequency stage.

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