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        Modulatory effect of dianthrone rich alcoholic flower extract of Cassia auriculata L. on experimental diabetes

        Syed Zameer Ahmed Khader,Sidhra Syed Zameer Ahmed,Senthil Kumar Balasubramanian,Thanga kumar Arunachalam,Geetha Kannappan,Mohamed Rafi Mahboob,Ponmurugan Ponnusamy,Kishore Ramesh 한국한의학연구원 2017 Integrative Medicine Research Vol.6 No.2

        Background: Diabetes is rapidly rising all over the world at an alarming rate and has changedfrom a mild disorder to major causes of mortality and morbidity in the youth and middle-aged people, and the prevalence is seen especially in six inhabited continents of the globe.The present study aims to explore the antidiabetic, lipid lowering effect of Cassia auriculataL. flowers in alloxan-induced diabetes.Methods: Diabetes was induced using alloxan monohydrate in experimental rats and sub-sequent therapeutic effects of C. auriculata extract and standard drug glibenclamide weremonitored. Bioassay-directed fractionation using silica gel column chromatography wasperformed until pure fractions were isolated. The effect of the treatment was analyzedby hematological parameters and enzyme assays. The pure compounds were confirmedwith thin layer chromatography and high performance liquid chromatography pattern andfurther subjected for characterization.Results: The alterations in blood glucose were monitored throughout the study. There wasa gradual fall in blood glucose and significant changes were observed in lipid profile andmetabolic enzyme after treatment with C. auriculata. Bioassay fractionation representedthat the C2 subfraction produced a dose-dependent fall in blood glucose and lipid profile andupon further purification yielded two pure compounds. The structure of the pure compoundwas elucidated using Fourier transform infrared,1H nuclear magnetic resonance,13C nuclearmagnetic resonance, and mass spectral data.

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