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RISS 인기검색어
- 검색키워드
- 저자 : Furka, Istvan (검색결과 5 건)
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Nemeth, Norbert,Baskurt, Oguz K.,Meiselman, Herbert J.,Furka, Istvan,Miko, Iren The Korean Society of Rheology 2009 Korea-Australia rheology journal Vol.21 No.3
Micropore filtration of dilute red blood cell (RBC) suspensions is a widely known method for determining red blood cell deformability. Use of this method for cells from various laboratory animal species does require considering the effects of the cell size to pore size ratio and of suspension hematocrit. In general, previous animal studies have utilized 5% hematocrit suspensions and five micron pores, and thus conditions similar to human clinical laboratory practice. However, when used for repeated sampling from small laboratory animals or for parallel multiple samples from different sites in large laboratory animals, the volume of blood sampled and hence the hematocrit of the test suspension may be limited. Our results indicate that hematocrit levels yielding stable values of RBC pore transit time are pore size and species specific: three micron pores = $2{\sim}5%$ for dog and $3{\sim}5%$ for rat; five micron pores $3{\sim}5%$ for dog and $1{\sim}5%$ for rat. An analytical approach using a common expression for calculating transit time is useful for determining the sensitivity of this time to hematocrit alterations and hence to indicate hematocrit levels that may be problematic.
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Norbert Nemeth,Oguz K. Baskurt,Herbert J. Meiselman,Istvan Furka,Iren Miko 한국유변학회 2009 Korea-Australia rheology journal Vol.21 No.3
Micropore filtration of dilute red blood cell (RBC) suspensions is a widely known method for determining red blood cell deformability. Use of this method for cells from various laboratory animal species does require considering the effects of the cell size to pore size ratio and of suspension hematocrit. In general, previous animal studies have utilized 5% hematocrit suspensions and five micron pores, and thus conditions similar to human clinical laboratory practice. However, when used for repeated sampling from small laboratory animals or for parallel multiple samples from different sites in large laboratory animals, the volume of blood sampled and hence the hematocrit of the test suspension may be limited. Our results indicate that hematocrit levels yielding stable values of RBC pore transit time are pore size and species specific: three micron pores=2~5% for dog and 3~5% for rat; five micron pores 3~5% for dog and 1~5% for rat. An analytical approach using a common expression for calculating transit time is useful for determining the sensitivity of this time to hematocrit alterations and hence to indicate hematocrit levels that may be problematic.
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Ferenc Kiss,Erika Sajtos,Lili Matyas,Zsuzsanna Magyar,Istvan Furka,Iren Miko,Norbert Nemeth 한국유변학회 2010 Korea-Australia rheology journal Vol.22 No.2
Viscosity of the suspending medium can be a determinant contributor to the effective measurements of red blood cell deformability using ektacytometry. Deformability of erythrocyte is known to show inter-species and gender differences, therefore the evaluation of the effects and the standardized usage of the suspending media are important in animal experiments. For evaluating the differences using various viscosity media, we aimed to test male and female Sprague-Dawley rats and beagle dogs. Blood samples were collected and red blood cell deformability was tested by a RheoScan-D200 slit-flow ektacytometer using parallel measurements in PVP solutions at viscosity of 15, 20 and 30mPa.s. The lowest elongation index values were measured in 15mPa.s solution in both species and genders. The inter-species differences were obvious: rats had higher elongation index values. Gender differences were found only in 20 and 30mPa.s samples: in rats males, while in dogs females had better red blood cell deformability. The standard usage of PVP solutions is essential for comparative studies, because the results obtained from different viscosity PVP solutions are not comparable to each other. In Sprague-Dawley rats and beagle dogs the relatively less viscous media (<20mPa.s) may result in less informative data.
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Norbert Nemeth,Oguz K. Baskurt,Herbert J. Meiselman,Ferenc Kiss,Mehmet Uyuklu,Timea Hever,Erika Sajtos,Peter Kenyeres,Kalman Toth,Istvan Furka,Iren Miko 한국유변학회 2009 Korea-Australia rheology journal Vol.21 No.2
Hemorheological results may be influenced by the time between blood sampling and measurement, and storage conditions (e.g., temperature, time) during sample delivery between laboratories may further affect the resulting data. This study examined possible hemorheological alterations subsequent to storage of rat and dog blood at room temperature (22℃) or with cooling (4~10℃) for 2, 4, 6, 24, 48 and 72 hours. Measured hemorheological parameters included hematological indices, RBC aggregation and RBC deformability. Our results indicate that marked changes of RBC deformability and of RBC aggregation in whole blood can occur during storage, especially for samples stored at room temperature. The patterns of deformability and aggregation changes at room temperature are complex and species specific, whereas those for storage at the lower temperature range are much less complicated. For room temperature storage, it thus seems logical to suggest measuring rat and dog cell deformability within 6 hours; aggregation should be measured immediately for rat blood or within 6 hours for dog blood. Storage at lower temperatures allows measuring EI up to 72 hours after sampling, while aggregation must be measured immediately, or if willing to accept a constant decrease, over 24~72 hours.
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Nemeth, Norbert,Baskurt, Oguz K.,Meiselman, Herbert J.,Kiss, Ferenc,Uyuklu, Mehmet,Hever, Timea,Sajtos, Erika,Kenyeres, Peter,Toth, Kalman,Furka, Istvan,Miko, Iren The Korean Society of Rheology 2009 Korea-Australia rheology journal Vol.21 No.2
Hemorheological results may be influenced by the time between blood sampling and measurement, and storage conditions (e.g., temperature, time) during sample delivery between laboratories may further affect the resulting data. This study examined possible hemorheological alterations subsequent to storage of rat and dog blood at room temperature ($22^{\circ}C$) or with cooling ($4{\sim}10^{\circ}C$) for 2, 4, 6, 24, 48 and 72 hours. Measured hemorheological parameters included hematological indices, RBC aggregation and RBC deformability. Our results indicate that marked changes of RBC deformability and of RBC aggregation in whole blood can occur during storage, especially for samples stored at room temperature. The patterns of deformability and aggregation changes at room temperature are complex and species specific, whereas those for storage at the lower temperature range are much less complicated. For room temperature storage, it thus seems logical to suggest measuring rat and dog cell deformability within 6 hours; aggregation should be measured immediately for rat blood or within 6 hours for dog blood. Storage at lower temperatures allows measuring EI up to 72 hours after sampling, while aggregation must be measured immediately, or if willing to accept a constant decrease, over 24~72 hours.
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