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- 저자 : Francisco Tenllado (검색결과 6 건)
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del Toro, Francisco,Fern?ndez, F?tima Tena,Tilsner, Jens,Wright, Kathryn M,Tenllado, Francisco,Chung, Bong Nam,Praveen, Shelly,Canto, Tomas APS Press 2014 Molecular plant-microbe interactions Vol.27 No.12
<P>Potyvirus HCPro is a multifunctional protein that, among other functions, interferes with antiviral defenses in plants and mediates viral transmission by aphid vectors. We have visualized in vivo the subcellular distribution and dynamics of HCPro from Potato virus Y and its homodimers, using green, yellow, and red fluorescent protein tags or their split parts, while assessing their biological activities. Confocal microscopy revealed a pattern of even distribution of fluorescence throughout the cytoplasm, common to all these modified HCPros, when transiently expressed in Nicotiana benthamiana epidermal cells in virus-free systems. However, in some cells, distinct additional patterns, specific to some constructs and influenced by environmental conditions, were observed: i) a small number of large, amorphous cytoplasm inclusions that contained α-tubulin; ii) a pattern of numerous small, similarly sized, dot-like inclusions distributing regularly throughout the cytoplasm and associated or anchored to the cortical endoplasmic reticulum and the microtubule (MT) cytoskeleton; and iii) a pattern that smoothly coated the MT. Furthermore, mixed and intermediate forms from the last two patterns were observed, suggesting dynamic transports between them. HCPro did not colocalize with actin filaments or the Golgi apparatus. Despite its association with MT, this network integrity was required neither for HCPro suppression of silencing in agropatch assays nor for its mediation of virus transmission by aphids.</P>
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A Model to Explain Temperature Dependent Systemic Infection of Potato Plants by Potato virus Y
최경산,Francisco del Toro,Francisco Tenllado,Tomas Canto,청봉남 한국식물병리학회 2017 Plant Pathology Journal Vol.33 No.2
The effect of temperature on the rate of systemic infectionof potatoes (Solanum tuberosum L. cv. Chu-Baek) by Potato virus Y (PVY) was studied in growthchambers. Systemic infection of PVY was observedonly within the temperature range of 16oC to 32oC. Within this temperature range, the time required for aplant to become infected systemically decreased from14 days at 20oC to 5.7 days at 28oC. The estimatedlower thermal threshold was 15.6oC and the thermalconstant was 65.6 degree days. A systemic infectionmodel was constructed based on experimental data,using the infection rate (Lactin-2 model) and the infectiondistribution (three-parameter Weibull function)models, which accurately described the completionrate curves to systemic infection and the cumulativedistributions obtained in the PVY-potato system, respectively. Therefore, this model was useful to predictthe progress of systemic infections by PVY in potatoplants, and to construct the epidemic models.
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A Model to Explain Temperature Dependent Systemic Infection of Potato Plants by Potato virus Y
Choi, Kyung San,Toro, Francisco del,Tenllado, Francisco,Canto, Tomas,Chung, Bong Nam The Korean Society of Plant Pathology 2017 Plant Pathology Journal Vol.33 No.2
The effect of temperature on the rate of systemic infection of potatoes (Solanum tuberosum L. cv. Chu-Baek) by Potato virus Y (PVY) was studied in growth chambers. Systemic infection of PVY was observed only within the temperature range of $16^{\circ}C$ to $32^{\circ}C$. Within this temperature range, the time required for a plant to become infected systemically decreased from 14 days at $20^{\circ}C$ to 5.7 days at $28^{\circ}C$. The estimated lower thermal threshold was $15.6^{\circ}C$ and the thermal constant was 65.6 degree days. A systemic infection model was constructed based on experimental data, using the infection rate (Lactin-2 model) and the infection distribution (three-parameter Weibull function) models, which accurately described the completion rate curves to systemic infection and the cumulative distributions obtained in the PVY-potato system, respectively. Therefore, this model was useful to predict the progress of systemic infections by PVY in potato plants, and to construct the epidemic models.
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정봉남,Tomas Canto,Francisco Tenllado,최경산,Jae Ho Joa,안정준,김준환,도기석 한국식물병리학회 2016 Plant Pathology Journal Vol.32 No.4
We examined the effects of temperature on acquisition of Potato virus Y-O (PVY-O), Potato virus A (PVA), and Potato leafroll virus (PLRV) by Myzus persicae by performing transmission tests with aphids that ac-quired each virus at different temperatures. Infection by PVY-O/PVA and PLRV increased with increasing plant temperature in Nicotiana benthamiana and Phy-salis floridan, respectively, after being transmitted by aphids that acquired them within a temperature range of 10–20oC. However, infection rates subsequently decreased. Direct qRT-PCR of RNA extracted from a single aphid showed that PLRV infection increased in the 10–20oC range, but this trend also declined shortly thereafter. We examined the effect of temperature on establishment of virus infection. The greatest number of plants became infected when N. benthamiana was held at 20oC after inoculation with PVY-O or PVA. The largest number of P. floridanaplants became in-fected with PLRV when the plants were maintained at 25oC. PLRV levels were highest in P. floridanakept at 20–25oC. These results indicate that the optimum temperatures for proliferation of PVY-O/PVA and PLRV differed. Western blot analysis showed that ac-cumulations of PVY-O and PVA coat proteins (CPs) were lower at 10oC or 15oC than at 20oC during early infection. However, accumulation increased over time. At 25oC or 30oC, the CPs of both viruses accumulated during early infection but disappeared as time passed. Our results suggest that symptom attenuation and re-duction of PVY-O and PVA CP accumulation at higher temperatures appear to be attributable to increased RNA silencing.
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Chung, Bong Nam,Canto, Tomas,Tenllado, Francisco,Choi, Kyung San,Joa, Jae Ho,Ahn, Jeong Joon,Kim, Chun Hwan,Do, Ki Seck The Korean Society of Plant Pathology 2016 Plant Pathology Journal Vol.32 No.4
We examined the effects of temperature on acquisition of Potato virus Y-O (PVY-O), Potato virus A (PVA), and Potato leafroll virus (PLRV) by Myzus persicae by performing transmission tests with aphids that acquired each virus at different temperatures. Infection by PVY-O/PVA and PLRV increased with increasing plant temperature in Nicotiana benthamiana and Physalis floridana, respectively, after being transmitted by aphids that acquired them within a temperature range of $10-20^{\circ}C$. However, infection rates subsequently decreased. Direct qRT-PCR of RNA extracted from a single aphid showed that PLRV infection increased in the $10-20^{\circ}C$ range, but this trend also declined shortly thereafter. We examined the effect of temperature on establishment of virus infection. The greatest number of plants became infected when N. benthamiana was held at $20^{\circ}C$ after inoculation with PVY-O or PVA. The largest number of P. floridana plants became infected with PLRV when the plants were maintained at $25^{\circ}C$. PLRV levels were highest in P. floridana kept at $20-25^{\circ}C$. These results indicate that the optimum temperatures for proliferation of PVY-O/PVA and PLRV differed. Western blot analysis showed that accumulations of PVY-O and PVA coat proteins (CPs) were lower at $10^{\circ}C$ or $15^{\circ}C$ than at $20^{\circ}C$ during early infection. However, accumulation increased over time. At $25^{\circ}C$ or $30^{\circ}C$, the CPs of both viruses accumulated during early infection but disappeared as time passed. Our results suggest that symptom attenuation and reduction of PVY-O and PVA CP accumulation at higher temperatures appear to be attributable to increased RNA silencing.
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