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Fengying Luo,Hang Chen,Wenjian Wei,Han Liu,Youzhong Chen,Shujiang Li 한국식물병리학회 2024 Plant Pathology Journal Vol.40 No.1
The aim of this study was to isolate biocontrol bacteria that could antagonize brown rot of <i>Dendrocalamus latiflorus</i>, optimize the culture conditions, and develop an effective biocontrol preparation for brown rot of <i>D. latiflorus</i>. This study isolated a bacterium with an antagonistic effect on bamboo brown rot from healthy <i>D. latiflorus</i> rhizosphere soil. Morphology, molecular biology, and physiological biochemistry methods identified it as <i>Bacillus siamensis</i>. The following culturing media and conditions improved the inhibition effect of <i>B. siamensis</i>: the best culturing media were 2% sucrose, 1.5% yeast extract, and 0.7% potassium chloride; the optimal culturing time, temperature, pH, and inoculation amount were 48 h, 30℃, 6, and 20%. The optimum formula of the applying bacterial suspension was 14% sodium dodecyl benzene sulfonate emulsifier, 4% Na<sub>2</sub>HPO<sub>4</sub>·2H<sub>2</sub>O, 0.3% hydroxypropyl methylcellulose thickener, and 20% <i>B. siamensis</i>. The pot experiment results showed the control effect of applying bacterial suspension, diluted 1,000 times is still better than that of 24% fenbuconazole suspension. The applying bacterial suspension enables reliable control of brown rot in <i>D. latiflorus</i>.