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- 저자 : Feiyi Shu (검색결과 1 건)
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Impact of Grafting Density on the Self-Assembly and Hydrophilicity of Succinylated Collagen
Wenxin Wang,Feiyi Shu,Lingyu Pan,Shanshan Huang,Xiao Tu,Ping Li,Siman Li,Yuanyuan Li,Chengzhi Xu,Yang Sun,Juntao Zhang,Haibo Wang 한국고분자학회 2020 Macromolecular Research Vol.28 No.6
Modification of protein could give their new functionality but would affect their intrinsic property and activity. In this paper, a series of succinylated collagen (SCol(n/1)) (n≥1) were prepared by modification of collagen with succinic anhydride at different molar ratio to amino groups amount of collagen. The impact of grafting density on the intrinsic self-assembly and additional hydrophilicity of succinylated collagen was explored. The results revealed that excessive grafting density of succinylated collagen would improve their hydrophilicity but weaken their self-assembly property, although the triple helix of collagen could be reserved after succinylation. SCol(1/1) (grafting density of 17%) with self-assembly property and good hydrophilicity was chosen to compare with native collagen. Compared to native collagen, thermostability of SCol(1/1) decreased slightly, however, SCol(1/1) could form softer hydrogel, which was more favorable for the proliferation of NIH/3 T3. The present work would help us to further understand the importance of grafting density for the design of modified collagen with intrinsic self-assembly property and additional new functionality.
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