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RISS 인기검색어
- 검색키워드
- 저자 : Eyad Al-Shehadah (검색결과 4 건)
- 무료
- 기관 내 무료
- 유료
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Transcriptome Analysis of the Barley-Rhynchosporium secalis Interaction
Al-Daoude, Antonious,Shoaib, Amina,Al-Shehadah, Eyad,Jawhar, Mohammad,Arabi, Mohammad Imad Eddin The Korean Society of Plant Pathology 2014 Plant Pathology Journal Vol.30 No.4
Leaf scald caused by the infection of Rhynchosporium secalis, is a worldwide crop disease resulting in significant loss of barley yield. In this study, a systematic sequencing of expressed sequence tags (ESTs) was chosen to obtain a global picture of the assembly of genes involved in pathogenesis. To identify a large number of plant ESTs, which are induced at different time points, an amplified fragment length polymorphism (AFLP) display of complementary DNA (cDNA) was utilized. Transcriptional changes of 140 ESTs were observed, of which 19 have no previously described function. Functional annotation of the transcripts revealed a variety of infection-induced host genes encoding classical pathogenesis-related (PR) or genes that play a role in the signal transduction pathway. The expression analyses by a semi-quantitative reverse transcription polymerase chain reaction (RT-PCR) revealed that Rar1 and Rpg4 are defense inducible genes, and were consistent with the cDNA-AFLP data in their expression patterns. Hence, the here presented transcriptomic approach provides novel global catalogue of genes not currently represented in the EST databases.
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Transcriptome Analysis of the Barley-Rhynchosporium secalis Interaction
Antonious Al-Daoude,Amina Shoaib,Eyad Al-Shehadah,Mohammad Jawhar,Mohammad Imad Eddin Arabi 한국식물병리학회 2014 Plant Pathology Journal Vol.30 No.4
Leaf scald caused by the infection of Rhynchosporium secalis, is a worldwide crop disease resulting in significant loss of barley yield. In this study, a systematic sequencing of expressed sequence tags (ESTs) was chosen to obtain a global picture of the assembly of genes involved in pathogenesis. To identify a large number of plant ESTs, which are induced at different time points, an amplified fragment length polymorphism (AFLP) display of complementary DNA (cDNA) was utilized. Transcriptional changes of 140 ESTs were observed, of which 19 have no previously described function. Functional annotation of the transcripts revealed a variety of infection-induced host genes encoding classical pathogenesis- related (PR) or genes that play a role in the signal transduction pathway. The expression analyses by a semi-quantitative reverse transcription polymerase chain reaction (RT-PCR) revealed that Rar1 and Rpg4 are defense inducible genes, and were consistent with the cDNA-AFLP data in their expression patterns. Hence, the here presented transcriptomic approach provides novel global catalogue of genes not currently represented in the EST databases.
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Pathogenic Groups Identified Among Isolates of Rhynchosporium secalis
Arabi, Mohammad Imad Eddin,Al-Shehadah, Eyad,Jawhar, Mohammad The Korean Society of Plant Pathology 2010 Plant Pathology Journal Vol.26 No.3
Scald, caused by Rhynchosporium secalis has been the major yield-reducing factor for barley production during the last decade. In this study, pathogenic groups of R. secalis were identified to obtain a global picture of the assembly of isolates involved in Syrian populations which is essential for the development of scald-resistant barley cultivars. To identify a number of pathogenic groups, 49 isolates collected over ten years from major barley growing areas in Syria were evaluated on five differential barley genotypes. Genotypes presented a continuous range of response from highly susceptible to moderately resistant, but none were immune to the disease. A cluster analysis placed isolates in six distinct differential pathogenic groups. Mean disease rating of 39.24% was the separation point between avirulent and virulent reactions. Isolate Rs46 exhibited distinct differential virulence patterns associated with high frequency across all genotypes. Hence, the data presented here provides crucial information for future selection of isolates to develop durable barley scald resistance.
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Pathogenic Groups Identified Among Isolates of Rhynchosporium secalis
Mohammad Imad Eddin Arabi,Eyad Al-Shehadah,Mohammad Jawhar 한국식물병리학회 2010 Plant Pathology Journal Vol.26 No.3
Scald, caused by Rhynchosporium secalis has been the major yield-reducing factor for barley production during the last decade. In this study, pathogenic groups of R. secalis were identified to obtain a global picture of the assembly of isolates involved in Syrian populations which is essential for the development of scald-resistant barley cultivars. To identify a number of pathogenic groups, 49isolates collected over ten years from major barley growing areas in Syria were evaluated on five differential barley genotypes. Genotypes presented a continuous range of response from highly susceptible to moderately resistant, but none were immune to the disease. A cluster analysis placed isolates in six distinct differential pathogenic groups. Mean disease rating of 39.24% was the separation point between avirulent and virulent reactions. Isolate Rs46 exhibited distinct differential virulence patterns associated with high frequency across all genotypes. Hence, the data presented here provides crucial information for future selection of isolates to develop durable barley scald resistance.
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