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정의민(Euimin Jung),이선아(Suna Lee),김남일(Namil Kim) 한국한의학연구원 2009 한국한의학연구원논문집 Vol.15 No.3
Sinpyeonjipsungmauibang is the classic on medical studies on horses written on the seventh year of Taejo of Chosun Dynasty, or the year of 1399. It was written with practical purpose, and it is the collection of medical studies on horses in our country from the last era of Koryo to the first era of Chosun. Acupuncture treatment in curing the disease of horses has much importance in this book. Most of the acupuncture was quoted from the classic Simuanjiji『司牧安驥集』 from the Dang Dynasty, China. However, the contents were selected to only include what we need, and it was supplemented with our own experiences and explanations to be put into practical use. The idea of acupuncture point and meridian can be found in the book; it is, however, not as clear and complete as the current system. Also, the needle used in the book was usually the “needle with fire(火針)” and “method of burning(烙法)”, and the “method of bloodletting(瀉血法)” was used often as well. The method of moxibustion was used as supplement for acupunture.
Metabolic engineering of E. coli for the production of glycosylated flavonoids
Dinesh Simkhada,EuiMin Kim,Nagendra Prasad Kurumbang,Tae-Jin Oh,Hei Chan Lee,Jae Kyung Sohng 한국당과학회 2008 한국당과학회 학술대회 Vol.2008 No.1
Glycosylation of flavonoid play crucial roles in stabilization of antocyanins and cyanidins; storage of flavonoid and terpenoids; and regulation of hormones. In addition, glycosylation has been recognized as one of the important mechanisms for detoxification of exogenous compounds. Here in this research, we have metabolically engineered the E. coli BL21DE3 (Δ pgi mutant) host to generate four different engineered host to produce glycosylated flavonoid. E. coli BL21DE3 (Δ pgi mutant) was engineered by integration of GalU, expression of CalS8 (dehydrogenase) and CalS9 (decarboxylase) together by cloning in pDuet/ampr vector and expression of four different 3-O-glycosyltransferase and 7-O- glycosyltransferase gene from Arabidopsis thaliana. Engineered hosts are expected to produce glucosyl as well as xylosyl glycosylated flavonoids which are characterized by HPLC as well as LC-MS analysis.
Metabolic Engineering of Escherichia coli for the Biological Synthesis of 7-O-Xylosyl Naringenin
Dinesh Simkhada,EuiMin Kim,Hei Chan Lee,송재경 한국분자세포생물학회 2009 Molecules and cells Vol.28 No.4
Flavonoids are a group of polyphenolic compounds that have been recognized as important due to their physio-logical and pharmacological roles and their health benefits. Glycosylation of flavonoids has a wide range of effects on flavonoid solubility, stability, and bioavailability. We previ-ously generated the E. coli BL21 (DE3) Δpgi host by delet-ing the glucose-phosphate isomerase (Pgi) gene in E. coli BL21 (DE3). This host was further engineered for whole-cell biotransformation by integration of galU from E. coli K12, and expression of calS8 (UDP-glucose dehydro-genase) and calS9 (UDP-glucuronic acid decarboxylase) from Micromonospora echinospora spp. calichensis and arGt-4 (7-O-glycosyltransferase) from Arabidopsis thaliana to form E. coli (US89Gt-4), which is expected to produce glycosylated flavonoids. To test the designed system, the engineered host was fed with naringenin as a substrate, and naringenin 7-O-xyloside, a glycosylated naringenin product, was detected. Product was verified by HPLC-LC/MS and ESI-MS/MS analyses. The reconstructed host can be applied for the production of various classes of glycosylated flavonoids.