http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
- 中文 을 입력하시려면 zhongwen을 입력하시고 space를누르시면됩니다.
- 北京 을 입력하시려면 beijing을 입력하시고 space를 누르시면 됩니다.
RISS 인기검색어
- 검색키워드
- 저자 : Ehrenfellner, Bianca (검색결과 1 건)
- 무료
- 기관 내 무료
- 유료
-
Postmortem proteomics to discover biomarkers for forensic PMI estimation
Choi, Kyoung-Min,Zissler, Angela,Kim, Eunjung,Ehrenfellner, Bianca,Cho, Eunji,Lee, Se-in,Steinbacher, Peter,Yun, Ki Na,Shin, Jong Hwan,Kim, Jin Young,Stoiber, Walter,Chung, Heesun,Monticelli, Fabio Ca Springer Berlin Heidelberg 2019 International journal of legal medicine Vol.133 No.3
<P>The assessment of postmortem degradation of skeletal muscle proteins has emerged as a novel approach to estimate the time since death in the early to mid-postmortem phase (approximately 24 h postmortem (hpm) to 120 hpm). Current protein-based methods are limited to a small number of skeletal muscle proteins, shown to undergo proteolysis after death. In this study, we investigated the usability of a target-based and unbiased system-wide protein analysis to gain further insights into systemic postmortem protein alterations and to identify additional markers for postmortem interval (PMI) delimitation. We performed proteomic profiling to globally analyze postmortem alterations of the rat and mouse skeletal muscle proteome at defined time points (0, 24, 48, 72, and 96 hpm), harnessing a mass spectrometry-based quantitative proteomics approach. Hierarchical clustering analysis for a total of 579 (rat) and 896 (mouse) quantified proteins revealed differentially expressed proteins during the investigated postmortem period. We further focused on two selected proteins (eEF1A2 and GAPDH), which were shown to consistently degrade postmortem in both rat and mouse, suggesting conserved intra- and interspecies degradation behavior, and thus preserved association with the PMI and possible transferability to humans. In turn, we validated the usefulness of these new markers by classical Western blot experiments in a rat model and in human autopsy cases. Our results demonstrate the feasibility of mass spectrometry–based analysis to discover novel protein markers for PMI estimation and show that the proteins eEF1A2 and GAPDH appear to be valuable markers for PMI estimation in humans.</P><P><B>Electronic supplementary material</B></P><P>The online version of this article (10.1007/s00414-019-02011-6) contains supplementary material, which is available to authorized users.</P>
연관 검색어 추천
이 검색어로 많이 본 자료
활용도 높은 자료
