야뇨증 환아들의 심리사회적 특성에 대한 다기관 연구 : 행동 및 정서 문제를 중심으로

조수철,김재원,신민섭,황준원,한상원,박관현,이상돈,김경도,김건석,서홍진,이유식,정재용,김영균,문두건,남궁미경,한창희,조원열,김영식,배기수,이종국,정우영,신의진 大韓神經精神醫學會 2005 신경정신의학 Vol.44 No.6

Objectives : The aim of this study was to examine the behavioral and emotional problems associated with nocturnal enuresis in Korean children. Methods : Three hundred eighteen children with nocturnal enuresis, together with their parents, completed the Child Behavior Checklist (CBCL), Disruptive Behavior Disorder Scale according to DSM-IV (DBDS), Children's Depression Inventory (CDI), State-Trait Anxiety Inventory for Children (STAIC), and Piers-Harris Children's Self-Concept Scale (PHCSC). Ninety-three normal students were selected as the control group. Results : Compared to the normal control group, the mean scores with regard to the withdrawn, social problems, attention problems, delinquent behavior, aggressive behavior, externalizing problems and total problems profiles were significantly higher in the nocturnal enuresis group according to the CBCL results. The nocturnal enuresis group also scored significantly higher in the ADHD and ODD profiles of the DBDS. The nocturnal enuresis group was more depressed and anxious than the control group according to the results of the CDI and STAI. The mean score of the PHCSC was significantly lower in the nocturnal enuresis group when compared to the normal control group. Conclusion : The results of this study suggest that children with nocturnal enuresis in Korea have clinically relevant behavioral and emotional problems. The findings support the link between nocturnal enuresis and psychopathology in Korean children.

다국어 질의응답을 위한 한국어 해석 시스템 설계 및 구현

강원석,황도삼 한국컴퓨터교육학회 2004 컴퓨터교육학회 논문지 Vol.7 No.4

다국어 질의 응답 시스템은 여러 언어의 질의에 대한 응답을 하는 시스템이다. LASSO 시스템은 다국어 질의응답 시스템 중의 하나이다. 본 논문은 LASSO 시스템을 위한 한국어 해석 새스템의 설계 및 구현에 관한 것이다. 질의 응답을 위한 한국어 해석 시스템은 한국어 질의를 처리할 수 있는 대화체 처리 기술이 필요하다. 그리고 다양한 분야의 질으에 대한 응답을 할 수 있는 범용의 시스템이어야 한다. 본 논문의 한국어 해석 시스템은 이와 같은 사항을 만족하기 위하여 심도 깊은 대화체 처리 기술보다 실용성이 높은 휴리스틱 규칙을 활용하였다. 이시스템은 다국어 질의 응답 시스템의 한국어 인터페이스 역할을 하는 것으로 질의 응답 시스템의 목적에 맞게 설계, 구현되었다. 본 해석 시스템에 적용된 기술은 정보검색 분야와 한국어 해석 분야에 응용할 수 있다. Multi-lingual query answering system is the system which answers on the queries with several languages. LASSO[1] is the system that aims to answer the multi-lingual query. In this paper, we design and implement a Korean analysis system for LASSO. The Korean analysis system for query answering needs processing techniques of dialogue style. And the system must be practical and general so as to use on various domains. This system uses not dialogue processing techniques with high cost and low utility but heuristic rules with low cost and high utility. It is designed and implemented as a Korean interface of multi-lingual query answering system. The techniques of this system highly contribute to information retrieval and Korean analysis researches.

태권도 남자경기의 체급별 공격기술 분석

김도호,황영성,조원민,차성웅 釜山大學校 附設 體育科學硏究所 1999 體育科學硏究所 論文集 Vol.15 No.-

The purpose of this study was to analyze attack frequency and scores of men(16 persons) Taekwondo players who joined the final games(32 games) of the 12th and 13th Taekwondo Championships The results obtained from this analysis were as follows; 1. In case of fin weight class, The total attack skill frequency of men players turned out to be 116 times, scores was 9 points and success rate was 7.76%. 2. In case of fly weight class, The total attack skill frequency of men players turned out to be 112 times, scores was 16 points and success rate was 13.11%. 3. In case of bantam weight class, The total attack skill frequency of men players turned out to be 149 times, scores was 16 points and success rate was 10.76%. 4. In case of feather weight class, The total attack skill frequency of men players turned out to be 101 times, scores was 9 points and success rate was 8.91%. 5. In case of light weight class, The total attack skill frequency of men players turned out to be 82 times, scores was 10 points and success rate was 12.20%. 6. In case of welter class, The total attack skill frequency of men players turned out to be 140 times, scores was 15 points and success rate was 10.71%. 7. In case of middle weight class, The total attack skill frequency of men players turned out to be 97 times, scores was 14 points and success rate was 14.43%. 8. In case of fin weight class, The total attack skill frequency of men players turned out to be 87 times, scores was 12 points and success rate was 13.79%.

대식세포의 Pro-inflammatory cytokine 생산에 대한 세포디짐의 효과

주성수,강희철,원태준,황광우,이도익 중앙대학교 약학연구소 2003 약학 논총 Vol.17 No.-

Cefodizime, a third cephalosporine, was originally developed for use in infectious disease as an advanced antibiotics. Cefodizime has also been known to play a meaningful role in activating natural killer properties of immune cells as well as enhancing immunological parameters such as phagocytosis. Furthermore, as the infection can result in remarkable changes for homeostatic mediators, it is expected that cefodizime may initiate the strong immuno- modualtory effect. In the present work, we investigated whether cefodizime has a biphasic effect of anti-bacteria and immunomodulation via macrophage. For the study, we isolated mouse peritoneal macrophages and treated with cefodizime 10㎍/㎖, 50㎍/㎖, 100㎍/㎖, IFN-v 10U/㎖.+LPS l㎍/㎖ Cells were then incubated for 4, 8, 12, and 24hrs to analysis the expression of IL-1, IL-6, IL-12 mRNA from macrophages. The results showed that cytokines were expressed more than that of control in a time-dependent manner and 100㎍/㎖ cefodizime group showed the greatest expression as much as the positive control (IFN-v+LPS)showed at 24h. In conclusion, cefodizime can play a significant role for the expression of pro-inflammatory cytokines, IL-1, IL-6, and IL-12 in earlier stage so that macrophages and other immune cells can response to the infectious substances, immediately. Moreover, when such pro-inflammatory cytokines are increased, we can also expect that the enhancement of cell-cell interaction of NK cell, CTL and B cell by cefodizime can affect the immunomodulatory effect along with its major role in anti-bacterial effect.

12-O-Tetradecanoylphorbol-13-Acetate에 의한 HL-60 세포 분화유도중 Vimentin 유전자 전사조절에 대한 AP-1의 역할

임규,김진희,권도원,김승민,이명선,윤경아,손미영,박종일,윤완희,황병두 忠南大學校 癌共同硏究所 1998 癌共同硏究所 硏究誌 Vol.2 No.1

Purpose: To gain insight on the role of AP-1 in transcriptional regulation of vimentin gene during differentiation of HL-60 cells by 12-O-tetradecanoylphorbol-13-acetate (TPA), the levels of vimentin mRNA and AP-1 have been investigated with Northern blot hybridization and DNA mobility shift assay. Materials and Methods: HL-60 cells were grown in RPMI 1640 medium supplemented with 10% heat-inactivated fetal calf serum and antibiotics in a humidified 5% CO_(2) at 37°C. Total RNA was prepared by a modification of the method of Karlinsey et al. Northern blot hybridization was performed by the method of Virca et al. EcoRI fragment of pVIM-GEM was used as probe for vimentin mRNA. DNA mobility shift assay was performed by the method of Lim et al. End labeled DNA probe(Upper strand, 5'-CGCTTGATGAGTCAGCCG- 3') for AP-1 binding activity was mixed with nuclear extracts in a 20 μl reaction volume containing 300 mM KC1, 60 mM HEPES, pH 7.9, 25 mM MgCl_(2), 1 mM EDTA, 1 mM DTT, 60% glycerol, and 2μg of poly[dI-dC]. Results: TPA increased vimentin mRNA levels, with maximal stimulation reached at 24 hr. The level of vimentin mRNA was induced in proportion to the concentration of TPA. TPA-induced vimentin mRNA was almost reduced by actinomycin-D pretreatment. TPA-induced stimulation of vimentin gene was completely reduced by staurosporin pretreatment. In DNA mobility shift assay, AP-1 newly appeared at 24 hr during TPA-induced differentiation and was almost not detected after the pretreatment of staurosporin. Conclusions: These results suggest that the induction of vimentin mRNA during TPA-dependent differentiation in HL-60 cells may be mediated by protein kinases C signal transduction and AP-1 is important to transcriptional regulation.

12-O-Tetradecanoylphorbol-13-Acetate에 의한 HL-60 세포 분화유도중 Vimentin 유전자 전사조절에 대한 AP-1의 역할

임규,김진희,권도원,김승민,이명선,윤경아,손미영,박종일,윤완희,황병두 충남대학교 생물공학연구소 1999 생물공학연구지 Vol.7 No.-

Purpose: To gain insight on the role of AP-1 in transcriptional regulation of vimentin gene during differentiation of HL-60 cells by 12-O-tetradecanoylphorbol-13-acetate (TPA), the levels of vimentin mRNA and AP-1 have been investigated with Northern blot hybridization and DNA mobility shift assay. Materials and Methods: HL-60 cells were grown in RPMI 1640 medium supplemented with 10% heat-inactivated fetal calf serum and antibiotics in a humidified 5% CO_2 at 37℃. Total RNA was prepared by a modification of the method of Karlinsey et al. Northern blot hybridization was performed by the method of Virca et al. EcoRI fragment of pVIM-GEM was used as probe for vimentin mRNA. DNA mobility shift assay was performed by the method of Lim et al. End labeled DNA probe(Upper strand, 5'-CGCTTGATGAGTCAGCCG- 3') for AP-1 binding activity was mixed with nuclear extracts in a 20 μl reaction volume containing 300 mM KCI, 60 mM HEPES, pH 7.9, 25 mM MgCI_2, 1 mM EDTA, 1 mM DTT, 60% glycerol, and 2 ㎍ of poly[dI-dC]. Results: TPA increased vimentin mRNA levels, with maximal stimulation reached at 24 hr. The level of vimentin mRNA was induced in proportion to the concentration of TPA. TPA-induced vimentin mRNA was almost reduced by actinomycin-D pretreatment. TPA-induced stimulation of vimentin gene was completely reduced by staurosporin pretreatment. In DNA mobility shift assay, AP-1 newly appeared at 24 hr during TPA-induced differentiation and was almost not detected after the pretreatment of staurosporin. Conclusions: These results suggest that the induction of vimentin mRNA during TPA-dependent differentiation in HL-60 cells may be mediated by protein kinases C signal transduction and AP-1 is important to transcriptional regulation.

GIS을 활용한 인공어초의 적지 선정에 관한 연구

김범규,황도현,윤홍주,서원찬,Kim, Bum-Kyu,Hwang, Do-Hyun,Yoon, Hong-Joo,Seo, Won-Chan 한국전자통신학회 2015 한국전자통신학회 논문지 Vol.10 No.5

This study carried out in order to investigate the most basic elements of suitability selection methods for composition of artificial reef. Acquired data by in-situ measurements and satellite remote sensing analysed in applying GIS. To identify the characteristic of marine environment around the West Sea, the South Sea and the East Sea of Korea, physical conditions-seabed sediment and depth, biological conditions-chlorophyll-${\alpha}$, chemical conditions-Sea Surface Temperature(SST) and DO were used. Suitable sites for artificial reef are selected Taean Peninsula, Geoje, Wando, Pohang, Seocheon, etc. From now on, it will be helpful to effectively utilize artificial reef as well as construct synthetic database. It is also expected to use basic data for artificial reef facilities management. 본 논문는 인공어초 조성의 가장 기본적인 요소인 적지선정을 위한 방법에 대해 고찰하였다. 현장관측과 위성 원격탐사(Remote Sensing, RS)로 획득한 자료를 지리정보시스템(Geographic Information System, GIS)을 활용하여 분석하였다. 한반도 주변해역의 해양환경 특성을 파악하기 위해 물리적 조건-해저저질, 수심, 생물적 조건-클로로필-${\alpha}$, 화학적 조건-해수온, DO를 활용하였다. 인공어초 적지로는 태안반도, 거제, 완도, 포항, 서천 등이 나타났다. 향후 본 연구는 인공어초 설치의 효율적인 활용뿐만 아니라 종합적인 데이터베이스의 구축에 도움을 줄 수 있을 것이다. 또한 인공어초 시설관리의 기초자료로 활용할 수 있을 것으로 기대된다.

실관막을 이용한 중금속 추출에서 물질전달에 미치는 분배비의 영향

李龍珍,金英一,陳道源,黃德基,朴東源 동아대학교 공과대학부설 생산기술연구소 1997 生産技術硏究所硏究論文集 Vol.2 No.1

The liquid-liquid extractions by use of microporous hollow fiber membrane modules are fast compared with conventional extraction equipment. Because they have a large surface area per volume. Solute diffuses out of the feed solution, through the fibers' porous wall, and into the extractant. The extractant and feed can be contacted at high speed, there are no problems with flooding, loading and channeling. Moreover, these modules should be characterized by low investment and operating costs because of the reduced hardware and the favorable hydrodynamics which minimizes concentration polarization and membrane fouling. In this paper, it was investigated the extraction selectivities for liquid-liquid extraction of heavy metal(Cd(Ⅱ)Co(II)) from dilute aqueous solution into the eatractants(EHPNA and TBP) by using the hydrophobic hollow fiber module. We also examined the effect of distribution ratio on the overall mass transfer coefficients.

Environmental Factors Affecting the Daily Catch Levels of<i>Anguilla japonica</i>Glass Eels in the Geum River Estuary, South Korea

Hwang, Sun Do,Lee, Tae Won,Choi, Il Su,Hwang, Sun Wan BioOne (Coastal Education and Research Foundation) 2014 JOURNAL OF COASTAL RESEARCH Vol.30 No.5

Neuron-Specific Fluorescence Reporter-Based Live Cell Tracing for Transdifferentiation of Mesenchymal Stem Cells into Neurons by Chemical Compound

Hwang, Do Won,Kwon, Hyun Woo,Jang, Jaeho,Jung, Hee Jung,Kim, Kwang Rok,Lee, Dong Soo Hindawi 2017 Stem cells international Vol.2017 No.-

<P>Although transdifferentiation of mesenchymal stem cells (MSCs) into neurons increases the possibility of therapeutic use of MSCs for neurodevelopmental disorders, the use of MSCs has the limitation on differentiation efficiency to neuronal lineage and lack of an easy method to monitor the transdifferentiation. In this study, using time-lapse live cell imaging, we assessed the neuronal differentiation of MSCs induced by a small molecule “NHPDQC (N-hydroxy-2-oxo-3-(3-phenylprophyl)-1,2-dihydroquinoxaline-6-carboxamide, C<SUB>18</SUB>H<SUB>17</SUB>N<SUB>3</SUB>O<SUB>3</SUB>).” Plasmid vector containing red fluorescence reporter genes under the control of the tubulin <I>α</I>1 (T<I>α</I>1) promoter (pT<I>α</I>1-DsRed2) traced the neuronal differentiation of MSCs. Two days after NHPDQC treatment, MSCs showed neuron-like phenotype with neurite outgrowth and high expression of neuron-specific markers in more than 95% cells. The fluorescence signals increased in the cytoplasm of pT<I>α</I>1-DsRed2-transfected MSCs after NHPDQC treatment. In vitro monitoring of MSCs along the time courses showed progressive increase of fluorescence till 30 h after treatment, corresponding with the increase in neurite length. We examined an efficient neuronal differentiation of MSCs by NHPDQC alone and monitored the temporal changes of neuronal differentiation by neuron-specific fluorescence reporter along time. This method would help further our understanding of the differentiation of MSCs to produce neurons by simple treatment of small molecule.</P>