Polymorphism, Expression of Natural Resistance-associated Macrophage Protein 1 Encoding Gene (NRAMP1) and Its Association with Immune Traits in Pigs

Ding, Xiaoling,Zhang, Xiaodong,Yang, Yong,Ding, Yueyun,Xue, Weiwei,Meng, Yun,Zhu, Weihua,Yin, Zongjun Asian Australasian Association of Animal Productio 2014 Animal Bioscience Vol.27 No.8

Natural resistance-associated macrophage protein 1 encoding gene (NRAMP1) plays an important role in immune response against intracellular pathogens. To evaluate the effects of NRAMP1 gene on immune capacity in pigs, tissue expression of NRAMP1 mRNA was observed by real time quantitative polymerase chain reaction (PCR), and the results revealed NRAMP1 expressed widely in nine tissues. One single nucleotide polymorphism (SNP) (ENSSSCG00000025058: g.130 C>T) in exon1 and one SNP (ENSSSCG00000025058: g.657 A>G) in intron1 region of porcine NRAMP1 gene were demonstrated by DNA sequencing and PCR-RFLP analysis. A further analysis of SNP genotypes associated with immune traits including contain of white blood cell (WBC), granulocyte, lymphocyte, monocyte (MO), rate of cytotoxin in monocyte (MC) and $CD4^-CD8^+$ T lymphocyte subpopulations in blood was carried out in four pig populations including Large White and three Chinese indigenous breeds (Wannan Black, Huai pig and Wei pig). The results showed that the SNP (ENSSSCG00000025058: g.130 C>T) was significantly associated with level of WBC % (p = 0.031), MO% (p = 0.024), MC% (p = 0.013) and $CD4^-CD8^+$ T lymphocyte (p = 0.023). The other SNP (ENSSSCG00000025058: g.657 A>G) was significantly associated with the level of MO% (p = 0.012), MC% (p = 0.019) and $CD4^-CD8^+$ T lymphocyte (p = 0.037). These results indicate that the NRAMP1 gene can be regarded as a molecular marker for genetic selection of disease susceptibility in pig breeding.

Relationship among porcine lncRNA TCONS_00010987, miR-323, and leptin receptor based on dual luciferase reporter gene assays and expression patterns

Ding, Yueyun,Qian, Li,Wang, Li,Wu, Chaodong,Li, DengTao,Zhang, Xiaodong,Yin, Zongjun,Wang, Yuanlang,Zhang, Wei,Wu, Xudong,Ding, Jian,Yang, Min,Zhang, Liang,Shang, Jinnan,Wang, Chonglong,Gao, Yafei Asian Australasian Association of Animal Productio 2020 Animal Bioscience Vol.33 No.2

Objective: Considering the physiological and clinical importance of leptin receptor (LEPR) in regulating obesity and the fact that porcine LEPR expression is not known to be controlled by lncRNAs and miRNAs, we aim to characterize this gene as a potential target of SSC-miR-323 and the lncRNA TCONS_00010987. Methods: Bioinformatics analyses revealed that lncRNA TCONS_00010987 and LEPR have SSC-miR-323-binding sites and that LEPR might be a target of lncRNA TCONS_00010987 based on cis prediction. Wild-type and mutant TCONS_00010987-target sequence fragments and wild-type and mutant LEPR 3'-UTR fragments were generated and cloned into pmiRRB-REPORT^TM-Control vectors to construct respective recombinant plasmids. HEK293T cells were co-transfected with the SSC-miR-323 mimics or a negative control with constructs harboring the corresponding binding sites and relative luciferase activities were determined. Tissue expression patterns of lncRNA TCONS_00010987, SSC-miR-323, and LEPR in Anqing six-end-white (AQ, the obese breed) and Large White (LW, the lean breed) pigs were detected by real-time quantitative polymerase chain reaction; backfat expression of LEPR protein was detected by western blotting. Results: Target gene fragments were successfully cloned, and the four recombinant vectors were constructed. Compared to the negative control, SSC-miR-323 mimics significantly inhibited luciferase activity from the wild-type TCONS_00010987-target sequence and wild-type LEPR-3'-UTR (p<0.01 for both) but not from the mutant TCONS_00010987-target sequence and mutant LEPR-3'-UTR (p>0.05 for both). Backfat expression levels of TCONS_00010987 and LEPR in AQ pigs were significantly higher than those in LW pigs (p<0.01), whereas levels of SSC-miR-323 in AQ pigs were significantly lower than those in LW pigs (p<0.05). LEPR protein levels in the backfat tissues of AQ pigs were markedly higher than those in LW pigs (p<0.01). Conclusion: LEPR is a potential target of SSC-miR-323, and TCONS_00010987 might act as a sponge for SSC-miR-323 to regulate LEPR expression.

Cascade Network Based Bolt Inspection In High-Speed Train

( Xiaodong Gu ),( Ji Ding ) 한국인터넷정보학회 2021 KSII Transactions on Internet and Information Syst Vol.15 No.10

The detection of bolts is an important task in high-speed train inspection systems, and it is frequently performed to ensure the safety of trains. The difficulty of the vision-based bolt inspection system lies in small sample defect detection, which makes the end-to-end network ineffective. In this paper, the problem is resolved in two stages, which includes the detection network and cascaded classification networks. For small bolt detection, all bolts including defective bolts and normal bolts are put together for conducting annotation training, a new loss function and a new boundingbox selection based on the smallest axis-aligned convex set are proposed. These allow YOLOv3 network to obtain the accurate position and bounding box of the various bolts. The average precision has been greatly improved on PASCAL VOC, MS COCO and actual data set. After that, the Siamese network is employed for estimating the status of the bolts. Using the convolutional Siamese network, we are able to get strong results on few-shot classification. Extensive experiments and comparisons on actual data set show that the system outperforms state-of-the-art algorithms in bolt inspection.

Coordinated Hospital-Home Fecal Microbiota Transplantation via Percutaneous Endoscopic Cecostomy for Recurrent Steroid-Dependent Ulcerative Colitis

( Xiaodong Ni ),( Shengxian Fan ),( Yongliang Zhang ),( Zhiming Wang ),( Lan Ding ),( Yousheng Li ),( Jieshou Li ) 대한소화기학회 2016 Gut and Liver Vol.10 No.6

Since its introduction as an alternative intestinal microbiota alteration approach, fecal microbiota transplantation (FMT) has been increasingly used as a treatment of choice for patients with ulcerative colitis (UC), but no reports exist regarding FMT via percutaneous endoscopic cecostomy (PEC). This report describes the case of a 24-year-old man with a 7-year history of recurrent, steroid-dependent UC. He received FMT via PEC once per day for 1 month in the hospital. After the remission of gastrointestinal symptoms, he was discharged from the hospital and continued FMT via PEC twice per week for 3 months at home. The frequency of stools decreased, and the characteristics of stools improved soon thereafter. Enteral nutrition was regained after 1 week, and an oral diet was begun 1 month later. Two months after the FMT end point, the patient resumed a normal diet, with formed soft stools once per day. The follow-up colonoscopy showed normal mucus membranes; then, the PEC set was removed. On the subsequent 12 months follow-up, the patient resumed orthobiosis without any gastrointestinal discomfort and returned to work. This case emphasizes that FMT via PEC can not only induce remission but also shorten the duration of hospitalization and reduce the medical costs; therefore, this approach should be considered an alternative option for patients with UC. (Gut Liver 2016;10:975-980)

Camphor wood waste-derived microporous carbons as high-performance electrode materials for supercapacitors

Ding Chenfeng,Yan Xiaodong,Lan Jin-le,유승곤,Yu Yunhua,Yang Xiaoping 한국탄소학회 2019 Carbon Letters Vol.29 No.3

Sustainable biomass-derived porous carbons demonstrate excellent capacitive properties owing to their heteroatom-rich nature and distinct textural feature. Herein, a series of nitrogen-/phosphorus-/oxygen-containing microporous carbons (CWW-N/P/O-MPCs) have been successfully fabricated by etching in H2O2 solution, pre-treatment of camphor wood wastes with KOH solution and subsequent carbonization. As an electrode material for supercapacitors, the typical microporous carbon (CWW-N/P/O-MPCs-0.5) exhibits a remarkably high specific capacitance of 245 F g−1 at 0.5 A g−1, corresponding to an impressively large volumetric capacitance of 208 F m−3, and excellent long-term stability over 10,000 cycles. The excellent electrochemical performance can be ascribed to the optimal combination of heteroatom groups and ultrafine micropores.

Camphor wood waste‑derived microporous carbons as high‑performance electrode materials for supercapacitors

Chenfeng Ding,Xiaodong Yan,Jin‑le Lan,Seungkon Ryu,Yunhua Yu,Xiaoping Yang 한국탄소학회 2019 Carbon Letters Vol.29 No.3

Sustainable biomass-derived porous carbons demonstrate excellent capacitive properties owing to their heteroatom-rich nature and distinct textural feature. Herein, a series of nitrogen-/phosphorus-/oxygen-containing microporous carbons (CWWN/ P/O-MPCs) have been successfully fabricated by etching in H2O2 solution, pre-treatment of camphor wood wastes with KOH solution and subsequent carbonization. As an electrode material for supercapacitors, the typical microporous carbon (CWW-N/P/O-MPCs-0.5) exhibits a remarkably high specific capacitance of 245 F g− 1 at 0.5 A g− 1, corresponding to an impressively large volumetric capacitance of 208 F m− 3, and excellent long-term stability over 10,000 cycles. The excellent electrochemical performance can be ascribed to the optimal combination of heteroatom groups and ultrafine micropores.

MiR-26a promotes apoptosis of porcine granulosa cells by targeting the 3β-hydroxysteroid-Δ24-reductase gene

Zhang, Xiaodong,Tao, Qiangqiang,Shang, Jinnan,Xu, Yiliang,Zhang, Liang,Ma, Yingchun,Zhu, Weihua,Yang, Min,Ding, Yueyun,Yin, Zongjun Asian Australasian Association of Animal Productio 2020 Animal Bioscience Vol.33 No.4

Objective: Apoptosis of ovarian granulosa cells (GCs) affects mammalian follicular development and fecundity. This study aimed to explore the regulatory relationship between microRNA-26a (miR-26a) and the 3β-hydroxysteroid-Δ24-reductase gene (DHCR24) gene in porcine follicular granular cells (pGCs), and to provide empirical data for the development of methods to improve the reproductive capacity of pigs. Methods: The pGCs were transfected with miR-26a mimic, miR-26a inhibitor and DHCR24-siRNA in vitro. The cell apoptosis rate of pGCs was detected by the flow cytometry. The secretion levels of estradiol (E2) and progesterone (P) in pGCs were detected by enzyme-linked immunosorbent assay. Double luciferase validation system was used to detect the binding sites between miR-26a and DHCR24 3'-UTR region. Qualitative real-time polymerase chain reaction and Western blotting were used to verify the DHCR24 mRNA and protein expression in pGCs, respectively, after transfecting with miR-26a mimic and miR-26a inhibitor. Results: Results showed that enhancement of miR-26a promoted apoptosis, and inhibited E2 and P secretion in pGCs. Meanwhile, inhibition of DHCR24 also upregulated the Caspase-3 expression, reduced the BCL-2 expression, promoted pGCs apoptosis, and inhibited E2 and P secretion in pGCs. There were the binding sites of miR-26a located within DHCR24 3'-UTR. Up-regulation of miR-26a inhibited DHCR24 mRNA and protein expression in pGCs. Conclusion: This study demonstrates that miR-26a can promote cell apoptosis and inhibit E2 and P secretion by inhibiting the expression of DHCR24 in pGCs.

Detrital-zircon geochronology and Hf isotope of Paleozoic sedimentary rocks in the Jilin Province, NE China: tectonic significance for microcontinental blocks of eastern Central Asian Orogenic Belt

Chenglong Shi,Xiaozhong Ding,Yanxue Liu,Xiaodong Zhou 한국지질과학협의회 2019 Geosciences Journal Vol.23 No.5

We undertook U-Pb-Hf isotope testing of detrital zircons from Late Paleozoic sedimentary rocks in the Jilin Province, NE China, with the aim of constraining the tectonic evolution of eastern Central Asian Orogenic Belt. The age populations of the detrital zircons from the sandstone of the Jiefangcun Formation in the Songliao Block are ~2234, ~1782, ~1030–921, ~817, ~540, and ~381–254 Ma, whereas those of the zircons from the greywacke of the Permian Kedao Formation in the Songliao Block yield are ~2436, ~1905, ~1692, 1146–1170, ~554–498, and ~342–255 Ma. Combined Hf isotope characteristics, these ages reveal that: (1) rocks from the Jiefangcun and Kedao formations were deposited after 254 and 255 Ma, respectively; (2) these formations were sourced mainly from the Songliao Block and the Jiamus Terrane in the eastern CAOB and to a lesser amount, from the northern margin of the NCC; and (3) it appears that several remnants of ancient Precambrian basement occur adjacent to these Late Paleozoic strata that occur within the microcontinental blocks. Detrital zircons from the silty sandstone of the Miaoling Formation in the western margin of the Khanka Terrane yield peak age populations of 508–476, 463–396, and 348–258 Ma, having similar εHf(t) values to those obtained from zircons within the Jiamusi Terrane. These data indicate that (1) deposition of the Miaoling Formation in the western margin of the Khanka Terrane had started by 258 Ma, and (2) these sediments were sourced mainly from the Jiamusi Terrane and local magmatic rocks. The widespread occurrence of detrital zircons with ages of 480–550 Ma and positive εHf(t) values within the Late Paleozoic strata in the Songliao Block and the Jiamusi–Khanka Terrane, indicates that the amalgamation of these blocks took place during the middle stage of the Paleozoic, probably after the Silurian, prior to the Late Permian.

Citrus Genetic Transformation with Interest Target Genes for Cultivar Improvement

Wenwu Guo,Yanxin Duan,Dingli Li,Xin Liu,Bin Tan,Xiaodong Cai,Xiuxin Deng 한국원예학회 2006 한국원예학회 기타간행물 Vol.- No.-

RNA Interference Targeting Nucleocapsid Protein Inhibits Porcine Reproductive and Respiratory Syndrome Virus Replication in Marc-145 Cells

Minnan Yang,Zhuang Ding,Qun Xiang,Xiaodong Zhang,Xiang Li,Seydou Sylla 한국미생물학회 2014 The journal of microbiology Vol.52 No.4

Porcine reproductive and respiratory syndrome (PRRS) is animportant disease, which leads to severe economic losses inswine-producing areas of the world. However, current antiviralstrategies cannot provide highly effective protection. In this study, three theoretically effective interference targetsites (71-91, 144-164, 218-238) targeting the nucleocapsid (N)gene of PRRSV were designed and selected, and then threesiRNA-expressing plasmids were constructed, respectivelynamed p2.1-N71, p2.1-N144, and p2.1-N218. The recombinantsiRNA-expressing plasmids were transfected into Marc-145 cells; then the cells were infected with PRRSV (JL07SWstrain); finally, after incubation for 48 h, the antiviral activityof those siRNA-expressing plasmids in Marc-145 cells wasassessed by cytopathic effects, virus titers, indirect immunofluorescence,and quantitative real-time PCR. Experimentalresults demonstrated that these three siRNA-expressing plasmidscould effectively and significantly inhibit the replicationof PRRSV by 93.2%, 83.6%, and 89.2% in Marc-145 cells,respectively. Among these three siRNA-expressing plasmids,p2.1-N71 was found to be most effective, while p2.1-N144and p2.1-N218 displayed relatively weak inhibition of virusreplication. The results indicated that siRNA-expressing plasmidstargeting the N gene of PRRSV could significantly inhibitPRRSV replication in Marc-145 cells. Based on our experimentalresults and previous reports, the 71-91, 179-197,and 234-252 sites of the N gene are good choices to effectivelyinhibit the replication of PRRSV, and this RNA interferencetechnique can be a potential anti-PRRSV strategy.