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- 저자 : Dimasuay, Kris Genelyn B. (검색결과 1 건)
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Microbiological Quality of Fresh Produce from Open Air Markets and Supermarkets in the Philippines
Vital, Pierangeli G.,Dimasuay, Kris Genelyn B.,Widmer, Kenneth W.,Rivera, Windell L. Hindawi Publishing Corporation 2014 The Scientific World Journal Vol.2014 No.-
<P>This study is the first in the Philippines to conduct a comprehensive assessment of the prevalence of bacterial pathogens and somatic phages in retailed fresh produce used in salad preparation, namely, bell pepper, cabbage, carrot, lettuce, and tomato, using culture and molecular methods. Out of 300 samples from open air and supermarkets, 16.7% tested positive for thermotolerant <I>Escherichia coli</I>, 24.7% for <I>Salmonella</I> spp., and 47% for somatic phages. Results show that counts range from 0.30 to 4.03 log<SUB>10</SUB> CFU/g for <I>E. coli</I>, 0.66 to ≥2.34 log<SUB>10</SUB> MPN/g for <I>Salmonella</I> spp., and 1.30 to ≥3.00 log<SUB>10</SUB> PFU/g for somatic phages. Statistical analyses show that there was no significant difference in the microbial counts between open air and supermarkets (<I>α</I> = 0.05). TaqMan and AccuPower Plus DualStar real-time polymerase chain reaction (RT-PCR) was used to confirm the presence of these organisms. The relatively high prevalence of microorganisms observed in produce surveyed signifies reduction in shelf-life and a potential hazard to food safety. This information may benefit farmers, consumers, merchants, and policy makers for foodborne disease detection and prevention.</P>
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