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Methyl Red: A Fluorescent Sensor for Hg2+ over Na+, K+, Ca2+, Mg2+, Zn2+, and Cd2+
Diganta Kumar Das,Priyanka Goswami,Champa Barman,Biva Das 대한환경공학회 2012 Environmental Engineering Research Vol.17 No.-
Methyl red (MR), a very well known acid base indicator (pKa = 5.2), shows fluorescence emission in the range 320 nm to 480 nm, with an emission maximum at 375 nm on excitation by photons at 310 nm. A fluorescent intensity titration of MR by Hg2+ ion, a well known toxic heavy metal ion, shows quenching of the fluorescent intensity of MR. The quenching continues till the concentration ratio between MR and Hg2+ ion becomes 1:1. Similar titration with a number of other metal ions, namely Na+, K+, Ca2+, Mg2+, Zn2+, and Cd2+, has very small effect on the fluorescent intensity of MR. Thus MR acts as a selective fluorescent sensor for Hg2+ ion by selective fluorescence quenching. The efficiency of fluorescence quenching value is found to be 8.0. The binding ratio and binding constant (β) between MR and Hg2+ ion are calculated from the fluorescence and UV/visible spectral data, and are found to be 1:1 and log β =4.68. The Hg:MR complex was found to be stable in the pH range 5.5 to 8.2.
Rana, Tanmoy,Bera, Asit Kumar,Das, Subhashree,Bhattacharya, Debasis,Pan, Diganta,Das, Subrata Kumar Asian Pacific Journal of Cancer Prevention 2016 Asian Pacific journal of cancer prevention Vol.17 No.9
Chronic arsenicosis is a major environmental health hazard throughout the world, including India. Animals and human beings are affected due to drinking of arsenic contaminated ground water, due to natural mineral deposits, arsenical pesticides or improperly disposed arsenical chemicals. Arsenic causes cancer with production of free radicals and reactive oxygen species (ROS) that are neutralized by an elaborate antioxidant defense system consisting of enzymes and numerous non-enzymatic antioxidants. Dietary antioxidant supplements are useful to counteract the carcinogenesis effects of arsenic. Oyster mushroom lectins can be regarded as ingredients of popular foods with biopharmaceutical properties. A variety of compounds have been isolated from mushrooms, which include polysaccharides and polysaccharopeptides with immune-enhancing effects. Lectins are beneficial in reducing arsenic toxicity due to anticarcinogenetic roles and may have therapeutic application in people suffering from chronic exposure to arsenic from natural sources, a global problem that is especially relevant to millions of people on the Indian subcontinent.