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Cunyu Li,Yun Ma,Xinglei Zhi,Guoping Peng 한국식품과학회 2023 Food Science and Biotechnology Vol.32 No.3
Gynostemma pentaphyllum saponin has a variety of biological properties. Classic separation methods of saponin, such as resin absorption and preparative chromatography are limited by environmental pollution and high cost. In the study, ultrasonic assisted membrane separation was firstly used to purify saponin from Gynostemma pentaphyllum. Total proteins, polysaccharides, saponin, gypenoside A and rutin were selected as indexes to optimize the pretreatment and purification parameters by response surface methodology. The fitted models were significant (p < 0.05) and the optimal conditions were: (1) removing protein and polysaccharides by MWCO 10,000 Da, ultrasonic power 400 W and pH 7.8; (2) separation flavonoids from saponin by MWCO 1000 Da, ultrasonic power 300 W and pH 7.9. The difficulty in separating saponin from flavonoids was solved by releasing flavonoids from micelles with ultrasonic assisted membrane method. The saponin content in Gynostemma pentaphyllum extracts reached 82.81%, which was more than four times of that obtained with resin adsorption method. The protective effect of saponins on SH-SY5Y cells injury induced by H2O2 was better than that of Gynostemma pentaphyllum extracts. The study suggested that ultrasonic assisted membrane method would be widely applied in the preparation of food materials.
Chong Li,Rong Min,Cunyue Lu,Chaodong Li 대한기계학회 2019 JOURNAL OF MECHANICAL SCIENCE AND TECHNOLOGY Vol.33 No.10
Wireless capsule endoscopy has the distinctive advantage of providing trustworthy results without substantial discomforts to patients. However, lack of capsule active motion control and energy supply have been significant obstacles preventing wider applications of this technology. To overcome these problems, this paper presents a micro piezoelectric motor with energy harvesting feature. The motor is driven by a single-phase voltage and can be used in capsule endoscopy. The stator was constructed by bonding two PZT plates (PZT 1 and PZT 2 ) onto the adjacent outer surfaces of a metal base. PZT 1 and PZT 2 can both be used to excite the in-plane bending modes of the stator to generate a traveling wave in the stator. The PZT plate supplied with no-exciting voltage source can be used to convert the superfluous vibration energy of stator body into electric energy to power small electronic devices. The elliptical trajectories of points on the stator surface result from a superposition of the two bending modes. The vibration and energy conversion characteristics of the stator were analyzed by using the finite element analysis software ANSYS. The structure parameters of the stator were adjusted until the mode mixture of the stator disappeared. A prototype motor was fabricated and tested to explore its mechanical and electric energy output abilities. The size of the prototype motor is about 9.8×9.2×22.0 mm 3 . The tested resonance frequencies of the operating modes are 15.305 kHz and 15.815 kHz, respectively. The maximum no-load speed is 85 rpm. The applied single-phase driving voltage is 100 V o-p (peak voltage) at frequency of 15.54 kHz. The stall torque is 0.98 mN·m, and the maximum output power of the harvesting PZT plate is 127 mW under an optimal equivalent load resistance of 5.16 kΩ.