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        Comparative transcriptome analyses of drought-resistant and - susceptible Brassica napus L. and development of EST-SSR markers by RNA-Seq

        Daojie Wang,Cuiling Yang,Long Dong,Jiacheng Zhu,Jianping Wang,Shufeng Zhang 한국식물학회 2015 Journal of Plant Biology Vol.58 No.4

        Brassica napus is a dicotyledonous plant in the family Brassicaceae. It is an important oil crop which has been widely cultivated over the world. However, drought stress is a very important threatening to B. napus production. In this study, two B. napus strains with different resistance to drought stress were treated with 200 g/L PEG-6000 as drought simulation agent (marked as S, R)and two controls were treated with 1/2 Hoagland medium(marked as ST, RT). With the help of Illumina paired-end RNA-seq technology and de novo assembly by Trinity, we obtained 107,294 Unigenes (integrated four sample results) with an average length of 834 bp and N50 1245. Of these Unigenes, 84,768, 92,545, 54,857, 28,129, 75,278 were assigned to NR, NT, Swiss-Prot, COG and GO database respectively. A total of 46,861 were mapped to 128 pathways by BLAST comparison against the KEGG database. In order to have an overall understanding of the unique genes’ expression difference among four samples, we utilized RPKM to calculate Unigene expression, and to identify differentially expressed genes. Besides, 22,414 SSRs were developed in this study.

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        Synthesis and characterization of novel Ti-containing mesoporous silicas support

        Peihong Yao,Weiwei Hu,Yan Bao,Cuiling Zhang,Bochao Zhu,Wenxia Liu,Yongxiao Bai 한양대학교 세라믹연구소 2011 Journal of Ceramic Processing Research Vol.12 No.3

        Titanium-containing novel mesoporous silicas have been synthesized at ambient temperature by a sol-gel method using amines with alkyl chains of 10-16 carbon atoms as a templating surfactant. An identical synthesis procedure was followed together with the addition of different amounts of titanium (IV) source in the synthesis gels to obtain its titanium-containing silicate gels. Following this synthesis route, it was possible to prepare materials without forming extrawall TiO2. The transparent gel formed as a precursor resulted in a relatively high titanium incorporation in the silicate framework of up to 0.4% Ti. The characterization results show that most Ti4+ are isolated and tetrahedrally incorporated into the framework of the silicate gel. Raman spectra and UV-vis spectra indicated that most Ti atoms were incorporated as isolated Ti sites into the silicate framework, and materials with a higher wt% Ti using relatively more coordination agent to prevent TiO2 forming anatase.

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