자외선의 조사간격이 브로일러 병아리의 볏 피부중 비타민 D₃함량에 미치는 영향

조인호,장윤환,이은택,여영수,배은경,김중달 한국축산학회 1994 한국축산학회지 Vol.36 No.1

This study was conducted to determine the content, of previtamin D₃(PreD₃), lumisterol₃(L₃), vitamin D₃(VD₃) and provitamin D₃(ProD₃) in comb skski of broiler chicks exposed to medium ware ultraviolet(UVB) lights in different interval. The broiler Hubbard line day old chicks(199 = 10 control + 3 irradiation interval × 9 elapsed time × 7 replica) were fed vitamin D deficient diet for 3 weeks in a windowless subdued light room and exposed to 297 ㎚ UVB light by 0.068 mJ/㎝-(10 min) three times in 0, 12 or 24 h interval. The comb skin were taken at 0, 6, 12, 18, 24, 48, 96, 144 or 240 h after last irradiation, and epidermis and dermis were separated. The lipid in sample was extracted by 9% ethyl acetate/hexane and purified by Sep-Pak silica catridge. The stright phase HPI-C was applied to analyze the concentration of Prop; and its photoproducts. When chicks were exposed once to UVB light for 30 min without interval, the mole % of ProD₃ in comb epidermis were 100% at control and 52.65% at 0 h after irradiation, thereafter it increased gradually to 88.17% at 240 h. PreD₃ and L₃ presented the maximum mole % at 0 h. VD₃ showed the peak value at 12 h. then decreased slowly. As UVB light was utilized to irradiate the chicks for 10 thin three times in 12 h interval, the ProD₃ mole portion in epidermis at 0 h was 76.4%, the lowest value among tested. PreD₃ and 1-3 preserved the highest level at 24 and 0 h, respectively, thereafter decreased gradully. VD₃ showed a peak at 6 h after exposure. When 24 h interval system was treated, the lowest value of ProD₃ 83.52% was appeared at 0 h. PreD₃ and L3 showed the highest level at 6 and 0 h, respectively. Mole ale of VD₃ had a peak value at 6 h and thin decreased. The mole % of ProD₃ and its photoproduets in comb dermis presented similar trends of time course variation as in those in epidermis. In respecting the method of UVB irradiation the PreD₃, L, and VDT were produced more quickly and largely in no intend system as compared to the time and amount produced in 12 or 24 h interval system.

Supplementation of oil-based inactivated H9N2 vaccine with M2e antigen enhances resistance against heterologous H9N2 avian influenza virus infection

Park, J.K.,Lee, D.H.,Cho, C.H.,Yuk, S.S.,To, E.O.,Kwon, J.H.,Noh, J.Y.,Kim, B.Y.,Choi, S.W.,Shim, B.S.,Song, M.K.,Lee, J.B.,Park, S.Y.,Choi, I.S.,Song, C.S. Elsevier Scientific Pub. Co 2014 Veterinary microbiology Vol.169 No.3

Avian influenza virus (AIV) subtype H9N2 has been evolving rapidly and vaccine escape variants have been reported to cause circulation of infections and economic losses. In the present study, we developed and evaluated ectodomain of the AIV matrix 2 (M2e) protein as a supplementing antigen for oil-based inactivated H9N2 vaccine to increase resistance against vaccine escape variants. AIV H9N2 M2e antigen was expressed in Escherichia coli and supplemented to inactivated H9N2 oil emulsion vaccine. Specific pathogen-free chickens received a single injection of inactivated H9N2 oil emulsion vaccines with or without M2e supplementation. At three weeks post vaccination, hemagglutination inhibition tests and enzyme-linked immunosorbent assays were performed to determine serological immune responses. Challenge study using a vaccine escape H9N2 variant was performed to evaluate the efficacy of M2e supplementation. M2e antigen supplemented in oil emulsion vaccine was highly immunogenic, and a single M2e-supplemented vaccination reduced challenge virus replication and shedding more effectively than non-supplemented vaccination.

자외선의 상이한 선량이 브로일러 병아리의 중족골 광물질 함량에 미치는 영향

조인호,장윤환,여영수,강훈석,김강수,배은경 한국축산학회 1994 한국축산학회지 Vol.36 No.2

This study was carried out to investigate the concentrations of Ca, P and ash in the metatarsus of broiler chicks exposed to 0.204 mJ/㎠ 30 min) or 0.409 mJ/㎠(60 min) of medium wave ultraviolet(UVB) fight. Eighty two Hubbard dayold broiler chicks(2 doses × 9 elapsed times × 4 replicate + 10 negative controls) were fed vitamin D(VD)deficient diets for 31 days in a windowless subdued light room. The metatarsus were collected at 0, 6, 12, 18, 30, 42, 66, 90, or 138 h after UVB irradiation. The metatarsus bones were separated from adhering tissue, ether extracted, dried and ashed(A.O.A.C., 1984). The Ca content in non-fat dry metatarsus was measured by atomic absorption spectrophotometry and P by urnmonium metavanadate colorimetry. The mean Ca concentration in metatarsus of negative control birds was 21 %, and those of chicks exposed to UVB light for 30 and 60 min were 30 and 38%, respectively with the maximum values at 42 h after irradiation. Thereafter, the Ca contents decreased throughout the l38 h period of this research. The mean P level of control chicks was 8.5%, and the level peaked at 15% (30 min treatment) and 11%(60 min) at 66 h after exposure. The decreasing trend continued later than 66 h. This mean ash content of the control group was 43% with the ash contents of the 0.204 mJ/㎠(30 min) and 0.409 mJ/㎠(60 min) groups decreasing slightly until 42 h, but increasing to 46%(30 min) and 47%(60 min) at l38 h after irradiation. This verified that Ca and P contents increased first and ash amount increased later in metatarsal bones of broiler chicks when they were exposed to 0.204 and 0.409 mJ/㎠ UVB light. Since there was not a big difference between minerals in metatarsal bones of chicks exposed to 0.204 or 0.409 mJ/㎠ UVB light, it was thought that further experiments would be necessary to elucidate the optimum dose of UVB light less than 0.204 mJ/㎠.

Attenuation of oxidative neuronal cell death by coffee phenolic phytochemicals

Cho, E.S.,Jang, Y.J.,Hwang, M.K.,Kang, N.J.,Lee, K.W.,Lee, H.J. Elsevier 2009 Mutation research Vol.661 No.1

Neurodegenerative disorders such as Alzheimer's disease (AD) are strongly associated with oxidative stress, which is induced by reactive oxygen species (ROS) including hydrogen peroxide (H<SUB>2</SUB>O<SUB>2</SUB>). Recent studies suggest that moderate coffee consumption may reduce the risk of neurodegenerative diseases such as AD, but the molecular mechanisms underlying this effect remain to be clarified. In this study, we investigated the protective effects of chlorogenic acid (5-O-caffeoylquinic acid; CGA), a major phenolic phytochemical found in instant decaffeinated coffee (IDC), and IDC against oxidative PC12 neuronal cell death. IDC (1 and 5μg/ml) or CGA (1 and 5μM) attenuated H<SUB>2</SUB>O<SUB>2</SUB>-induced PC12 cell death. H<SUB>2</SUB>O<SUB>2</SUB>-induced nuclear condensation and DNA fragmentation were strongly inhibited by pretreatment with IDC or CGA. Pretreatment with IDC or CGA also inhibited the H<SUB>2</SUB>O<SUB>2</SUB>-induced cleavage of poly(ADP-ribose) polymerase (PARP), and downregulation of Bcl-X<SUB>L</SUB> and caspase-3. The accumulation of intracellular ROS in H<SUB>2</SUB>O<SUB>2</SUB>-treated PC12 cells was dose-dependently diminished by IDC or CGA. The activation of c-Jun N-terminal protein kinase (JNK) and p38 mitogen-activated protein kinase (MAPK) by H<SUB>2</SUB>O<SUB>2</SUB> in PC12 cells was also inhibited by IDC or CGA. Collectively, these results indicate that IDC and CGA protect PC12 cells from H<SUB>2</SUB>O<SUB>2</SUB>-induced apoptosis by blocking the accumulation of intracellular ROS and the activation of MAPKs.

Inhibitory effects of sulfur compounds on methane oxidation by a methane-oxidizing consortium

Lee, E.H.,Moon, K.E.,Kim, T.G.,Lee, S.D.,Cho, K.S. Society for Bioscience and Bioengineering, Japan ; 2015 Journal of bioscience and bioengineering Vol.120 No.6

Kinetic and enzymatic inhibition experiments were performed to investigate the effects of methanethiol (MT) and hydrogen sulfide (H<SUB>2</SUB>S) on methane oxidation by a methane-oxidizing consortium. In the coexistence of MT and H<SUB>2</SUB>S, the oxidation of methane was delayed until MT and H<SUB>2</SUB>S were completely degraded. MT and H<SUB>2</SUB>S could be degraded, both with and without methane. The kinetic analysis revealed that the methane-oxidizing consortium showed a maximum methane oxidation rate (V<SUB>max</SUB>) of 3.7 mmol g-dry cell weight (DCW)<SUP>-1</SUP> h<SUP>-1</SUP> and a saturation constant (K<SUB>m</SUB>) of 184.1 μM. MT and H<SUB>2</SUB>S show competitive inhibition on methane oxidation, with inhibition values (K<SUB>i</SUB>) of 1504.8 and 359.8 μM, respectively. MT was primary removed by particulate methane monooxygenases (pMMO) of the consortium, while H<SUB>2</SUB>S was degraded by the other microorganisms or enzymes in the consortium. DNA and mRNA transcript levels of the pmoA gene expressions were decreased to ~10<SUP>6</SUP> and 10<SUP>3</SUP>pmoA gene copy number g-DCW<SUP>-1</SUP> after MT and H<SUB>2</SUB>S degradation, respectively; however, both the amount of the DNA and mRNA transcript recovered their initial levels of ~10<SUP>7</SUP> and 10<SUP>5</SUP>pmoA gene copy number g-DCW<SUP>-1</SUP> after methane oxidation, respectively. The gene expression results indicate that the pmoA gene could be rapidly reproducible after methane oxidation. This study provides comprehensive information of kinetic interactions between methane and sulfur compounds.

Rapid expression of RASD1 is regulated by estrogen receptor-dependent intracellular signaling pathway in the mouse uterus

Kim, H.R.,Cho, K.S.,Kim, E.,Lee, O.H.,Yoon, H.,Lee, S.,Moon, S.,Park, M.,Hong, K.,Na, Y.,Shin, J.E.,Kwon, H.,Song, H.,Choi, D.H.,Choi, Y. North-Holland 2017 Molecular and cellular endocrinology Vol.446 No.-

<P>Dexamethasone-induced RAS-related protein 1 (RASD1) is a signaling protein that is involved in various cellular processes. In a previous study, we found that RASD1 expression was down-regulated in the uterine endometrium of repeated implantation failure patients. The study aim was to determine whether RASD1 is expressed in the endometrium of mouse uterus and how it is regulated by steroid hormones during the estrous cycle. In this study, we investigated RASD1 expression and regulation in an ovariectomized female mouse model. Rasdl mRNA was highly expressed in mouse reproductive tissues, including the uterus. Rasdl expression was detected exclusively in the endometrial epithelium at the proestrus stage of the estrous cycle. Rasd1 expression in uteri increased with administration of estradiol, but not progesterone. Its expression was rapidly induced within 2 h after E2 treatment. Pretreatment with ICI 182,780, an estrogen receptor antagonist, reduced RASD1 protein expression. In addition, we identified that rapid expression of Rasd1 was mediated by the estrogen intracellular signaling including both p38-mitogen-activated protein kinase and the extracellular signal-regulated kinase. These findings suggest that RASD1 acts as a novel signaling molecule and plays an important role in regulating dynamic uterine remodeling during the estrous cycle in the uterus. (C) 2017 Elsevier B.V. All rights reserved.</P>

Role of n-type seed-layers in microstructural evolution of intrinsic nanocrystalline silicon and solar cell performance

Lee, J.E.,Ahn, S.,Park, J.H.,Yoo, J.,Yoon, K.H.,Kim, D.,Cho, J.S. Elsevier 2013 Current Applied Physics Vol.13 No.7

Nanocrystalline silicon (nc-Si:H) thin-film n-i-p solar cells were constructed on flexible stainless steel substrates by plasma-enhanced chemical vapor deposition. Influence of the n-type seed-layer on the microstructural evolution of the subsequent intrinsic nc-Si:H absorbers and the resultant performance of nc-Si:H solar cells was investigated. The crystalline volume fraction of the seed-layer can be effectively controlled by varying the hydrogen (H<SUB>2</SUB>) to silane (SiH<SUB>4</SUB>) gas flow ratio. Defect-dense amorphous regions were observed at the initial growth stage of the i-layers deposited on low crystalline volume fraction (X<SUB>c</SUB><SUP>n</SUP>) n-type seed-layers. Increasing the X<SUB>c</SUB><SUP>n</SUP> reduced the amorphous region at the n/i interface of the i nc-Si:H layers, evidenced by Raman scattering and transmission electron microscopy (TEM) measurements. Elimination of the defect-rich amorphous region within the i-layer by depositing the nc-Si:H solar cells on highly crystalline seed-layer caused significant improvements in the short circuit current density (J<SUB>sc</SUB>) and fill factor (FF). This is mainly due to the enhancement of long-wavelength light response and extraction efficiency of photo-carrier charges. The nc-Si:H solar cells prepared on a highly crystalline seed-layer (X<SUB>c</SUB><SUP>n</SUP>=73%) exhibited a 65.6% higher conversion efficiency than those on the n-type amorphous layers (X<SUB>c</SUB><SUP>n</SUP>=0%).

Divergences in morphological changes and antioxidant responses in salt-tolerant and salt-sensitive rice seedlings after salt stress

Lee, M.H.,Cho, E.J.,Wi, S.G.,Bae, H.,Kim, J.E.,Cho, J.Y.,Lee, S.,Kim, J.H.,Chung, B.Y. Gauthier-Villars ; Elsevier Science Ltd 2013 Plant physiology and biochemistry Vol.70 No.-

Salinization plays a primary role in soil degradation and reduced agricultural productivity. We observed that salt stress reversed photosynthesis and reactive oxygen scavenging responses in leaves or roots of two rice cultivars, a salt-tolerant cultivar Pokkali and a salt-sensitive cultivar IR-29. Salt treatment (100 mM NaCl) on IR-29 decreased the maximum photochemical efficiency (Fv/Fm) and the photochemical quenching coefficient (qP), thereby inhibiting photosynthetic activity. By contrast, the salt treatment on Pokkali had the converse effect on Fv/Fm and qP, while increasing the nonphotochemical quenching coefficient (NPQ), thereby favoring photosynthetic activity. Notably, chloroplast or root cells in Pokkali maintained their ultrastructures largely intact under the salt stress, but, IR-29 showed severe disintegration of existing grana stacks, increase of plastoglobuli, and swelling of thylakoidal membranes in addition to collapsed vascular region in adventitious roots. Pokkali is known to have higher hydrogen peroxide (H<SUB>2</SUB>O<SUB>2</SUB>)-scavenging enzyme activities in non-treated seedlings, including ascorbate peroxidase, catalase, and peroxidase activities. However, these enzymatic activities were induced to a greater extent in IR-29 by the salt stress. While the level of endogenous H<SUB>2</SUB>O<SUB>2</SUB> was lower in Pokkali than in IR-29, it was reversed upon the salt treatment. Nevertheless, the decreased amount of H<SUB>2</SUB>O<SUB>2</SUB> in IR-29 upon the salt stress didn't result in a high scavenging activity of total cell extracts for H<SUB>2</SUB>O<SUB>2</SUB>, as well as O<SUB>2</SUB>?<SUP>-</SUP> and ?OH species. The present study suggests that the tolerance to the moderate salinity in Pokkali derives largely from the constitutively maintained antioxidant enzymatic activities as well as the induced antioxidant enzyme system.

Association and functional relevance of E237G, a polymorphism of the high-affinity immunoglobulin E-receptor β chain gene, to airway hyper-responsiveness

Kim, Y.-K.,Park, H.-W.,Yang, J.-S.,Oh, S.-Y.,Chang, Y.-S.,Shin, E.-S.,Lee, J.-E.,Kim, S.,Gho, Y. S.,Cho, S.-H.,Min, K.-U.,Kim, Y.-Y. Blackwell Scientific Publications 2007 Clinical and experimental allergy Vol.37 No.4

<P>Summary</P><P>Background</P><P>The hyper-sensitivity reaction of IgE, with its high-affinity receptors (FcϵRI), is central to the phenomenon of atopic diseases.</P><P>Objective</P><P>To evaluate the genetic effects of non-synonymous single-nucleotide polymorphisms (SNPs) of FcϵRI on intermediate phenotypes of asthma, i.e. atopy and airway hyper-responsiveness (AHR), in the Korean general population.</P><P>Subjects and methods</P><P>Atopy and AHR were evaluated in a cohort of 2055 subjects, aged 10–18 years, using skin prick tests (SPTs) for common aeroallergens and total serum IgE and methacholine bronchial provocation tests. All FcϵRI-α, FcϵRI-β, and FcϵRI-γ gene exons of 24 healthy subjects were sequenced to locate informative non-synonymous SNPs (minor allele frequency >2%). Informative SNPs were then scored, using the high-throughput single base extension method. Relative risk (RR) was determined by multiple logistic regression analysis, after adjusting for confounding factors. The functional relevance of non-synonymous SNPs was analysed using the sorting intolerant from tolerant (SIFT) program.</P><P>Results</P><P>The SNP search found only one informative non-synonymous SNP in FcϵRI-β: E237G (minor allele frequency=0.21). The positive rate of AHR was lower among subjects with the 237<SUP>*</SUP>E allele than among those with 237<SUP>*</SUP>G [RR (95% confidence interval)=0.41 (0.19–0.89); <I>P</I>=0.01]. However, the E237G substitution was not associated with either a positive SPT response or total serum IgE levels. Sequence evolution analysis predicted that the E237G variation is an intolerant amino acid substitution, with functional importance.</P><P>Conclusion</P><P>In the Korean general population, AHR is significantly associated with the E237G polymorphism of FcϵRI-β, which results in an intolerant amino acid substitution.</P>

Characterization of Interface States in MOS Systems by Using Photonic High-Frequency Capacitance-Voltage Responses

S.J.Song,H.T.Kim,S.S.Chi,M.S.Kim,W.S.Chang,S.D.Cho,H.T.Shin,T.E.Kim,H.J.Kang,D.J.Kim,D.M.Kim 한국물리학회 2002 THE JOURNAL OF THE KOREAN PHYSICAL SOCIETY Vol.41 No.6

Based on the photonic high-frequency capacitance-voltage response of Metal-Oxide-Semiconductor capacitors, we report an improved characterization method for the analyzing of interface states in MOS systems. An optical source with a photonic energy $E_{ph}$ = 0.943 eV ($\lambda$ = 1314.5 nm) is employed for photonic deep-depletion (fast sweep rate) high-frequency Capacitance-Voltage (photonic DD HF-CV) characterization of interface states distributed in the photo-responsive energy band. Using the photonic DD HF-CV characterization, we obtained a U-shaped distribution of $D_{it}$ over ($E_V + E_g/2 - q\phi_f) < E_t < (E_V + E_g/2 - q\phi_f + q\phi_S$) for N-type Metal-Oxide-Semiconductor capacitors and ($E_C - E_g/2 - q\phi_f + q\phi_S) < E_t < (E_C - E_g/2 - q\phi_f$) for P-type Metal-Oxide-Semiconductor capacitors.