Development of Methodologies and Techniques for Transfer of Queen Bees and Influx

Cheon Rak Kwon,In Seck Kim,Su Jin Lee,Gyeong Choi 한국응용곤충학회 2018 한국응용곤충학회 학술대회논문집 Vol.2018 No.04

본 연구는 서양종꿀벌 여왕벌을 택배나 차량 등으로 이동 시킬 때, 여왕벌의 폐사, 훼손 등을 줄이고 농가의 벌통에 잘 유입하여 여왕벌이 정상적으로 산란할 수 있는 최적의 방법을 찾고자, 2013년 5월 25일부터 8월 2일까지 경북 예천군 소재 2개 양봉농가에서 여왕벌 60마리를 대상으로 총 3회에 걸쳐 수행되었다. 여왕벌 이동기구는 양봉농가 및 국외 연구소에서 주로 사용하고 있는 소형왕롱과 소형벌통 2종류를 대상으로 시험 하였다. 시험 결과, 1~2일 정도의 짧은 기간에는 이동왕롱의 종류에 따라 여왕벌의 생존율 및 산란일에서 차이가 없었다. 유입 성공율을 보면, 소형왕롱으로 이동시킨 여왕벌은 30% 정도의 유입에 실패 하였으나, 소형벌통은 유입실패율이 10% 이하로 나타났다. 산란능력 검사를 위한 봉개된 육아방수 조사에서는, 소형벌통을 이용한 여왕벌 유입군이 소형왕롱을 이용한 여왕벌 유입군 보다 봉개된 육아방수가 4~75%까지 많게 나타났다. 한편 소형벌통은 소비 1매를 그대로 무왕군에 합봉하여 모두 여왕벌 유입이 잘 되었으나, 소형왕롱을 이용한 여왕벌의 직접 소문유입의 경우 여왕벌이 일벌의 공격을 여러 마리가 죽었다. 따라서 소형왕롱을 저밀소비에 하루 정도 부착하여 두어다가 여왕벌을 풀어주는 간접유입이 더 효과적이며, 유밀기, 봉충소비의 발췌, 먹이의 풍부, 무왕군의 벌세력 등이 유입 성공율에 영향을 미쳤다.

섬과 내륙지역의 기상 환경에 따른 교미 여왕벌 생산 효율 규명

이수진(Su Jin Lee),권천락(Cheon Rak Kwon),김인석(In Seok Kim),윤광순(Gwang Sun Youn),이병일(Byeong Il Lee),이장범(Jang Beom Lee),최경(Gyeong Choi),최효열(Hyo Yeol Choi),홍호진(Ho Jin Hong) 한국양봉학회 2014 韓國養蜂學會誌 Vol.29 No.4

The ideal mating season of queen bees and the production efficiency of mating queen bees in island and inland areas were explored on an island called Wido which is 15km away from an inland region. This research was conducted so as to preserve honeybee lineages. In order to compare a mating season of queen bees on an island with that in an inland area, 10 drone colonies, 10 organized queenless colonies, and 60 queen bees were raised. The rates of grafting queen cells, the rates of oviposition, and the priod needed for the first oviposition was examined from March 26th to May 20th. In addition, with a view to find out an ideal mating season of queen bees in an island, 10 drone colonies, 10 organized queenless colonies, and 480 queen bees were raised. The rates of grafting queen cells, the rates of oviposition and the number of days needed for the first oviposition were examined on the basis of daily and monthly weather environments. Because of weather differences between island and inland areas, research on the mating season of queen bees resulted in different rates of grafting queen cells as follows; 93% on the island, and 100% in the inland area. This finding resulted from a lower temperature and stronger winds in an island. The rate of oviposition on the island was 50% and that of the inland area was 86.7%. The priod needed for the first oviposition was 21 days on the island and 10 days in an inland area, respectively, which shows a huge discrepancy between them. The first oviposition dates on the island were all on May 1st, but those in the inland area varied depending on time and seasons. As for the perfect mating season of queen bees, the rates of grafting queen cells were 91% in the first experiment, 96% in the second, and 98% in the third. Meanwhile, the rates of oviposition were 37% in the first, 63% in the second, and 71% in the third, which indicates large differences, depending on maximum temperature and wind speed. The numbers of days needed for the first oviposition were 27 days in the first, 21 days in the second, and 16 days in the third. The later dates of grafting queen cells was getting, the shorter the priod needed for the first oviposition weas. The oviposition dates were all on May 1st, as mentioned above. In conclusion, even though the rate of oviposition on the island was 36% lower than that of the inland area, islands would be better for isolated mating spaces for the purpose of preserving honeybee lineages. According to the findings of these experiments, when islands are used as isolated mating spaces, it is advisable that mating should be done under the following conditions; wind speed less than 1.5m/s, maximum temperature above 20°C and moving hives should be done after April 25th.

사상체질 병증별 월경 양상의 임상 연구

임성락,김구,오승윤,권영미,주종천,Lim, Sung-Rak,Kim, Koo,Oh, Seung-Yun,Kwon, Young-Mi,Joo, Jong-Cheon 사상체질의학회 2009 사상체질의학회지 Vol.21 No.1

1. Objective To investigate characteristics of menstrual patterns according to Sasang Constitutional disease, and to utilize for diagnosis and treatment of Sasang Constitutional disease. 2. Method The recruited subjects were 430 women who convinced their Sasang Constitution by taking herbal medicine and improving of chief complaint. The items of data contain age at menarche, age at menopause, menstrual cycle, menstrual duration, cyclic regularity of menstruation, severity of menstrual pain, the worst day of menstrual pain, type of menstrual pain, amount of menstruation. 3. Results 1) Severity of menstrual pain, the worst day of menstrual pain, menstrual pain accompanied with nausea were significant among Sasang Constitutional diseases. 2) In Bae-chu-pyo-byeong disease (背椎表病證), Wi-wan-han disease (胃院寒證), So-eum disease (少陰證) and Tae-eum disease (太陰證), menstrual pain was severer than other diseases. 3) In Hae-yeok disease and So-yang-sang-pung disease (少陽傷風證), the worst day of menstrual pain was a day before menstruation. In Hyung-gyeok-yeol disease (胸膈熱證), Tae-eum disease (太陰證), So-eum disease (少陰證), the frequency of a day after menstruation in the worst day of menstrual pain was higher than other diseases. In Wi-wan-han disease (胃院寒證), Mang-yang disease (亡陽證), the frequency of two day after menstruation in the worst day of menstrual pain was higher than other diseases. 4) In So-yang-sang-pung disease (少陽傷風證), Mang-eum disease (亡陰證), Eum-heo-o-yeol diasese (陰虛午熱證), Ul-gwang disease (鬱狂證), Tae-eum disease (太陰證), So-eum disease (少陰證), the frequency of menstrual pain accompanied with nausea were higher than other diseases. 4. Conclusion Menstrual patterns, especially menstrual pain is different among Sasang Constitutional diseases. Assessing menstrual pain can be used for diagnosing Sasang Constitutional diseases, deciding treatment course, determinating prognosis.

왕사마귀(Tenodera aridifolia)의 발육 및 산란 특성

문형철,임주락,권석주,소순영,전형권,Moon, Hyung cheol,Lim, Ju Rak,Kwon, Suk Ju,So, Sun Young,Cheon, Hyung Kwon 한국잠사학회 2019 한국잠사곤충학회지 Vol.55 No.1

왕사마귀 실내 사육을 위하여 발육 및 산란 특성을 조사한 결과, 왕사마귀 약충은 5 ~ 6회 탈피 후 성충으로 우화하였으며, 6회 탈피하는 약충의 비율은 73.5~77.3%이었다. 왕사마귀 약충 기간은 25, 28, $30^{\circ}C$에서 각각 71.9일, 53.5일, 54.7일로 약충 발육적온은 $28{\sim}30^{\circ}C$이었으며, 탈피 횟수에 따른 온도별 약충 총 발육기간은 차이가 없었다. 왕사마귀의 알 기간은 $25^{\circ}C$에서 41.3일이었다. 왕사마귀 암컷 수명은 평균 82.0일로 평균 3.2개의 난괴를 낳았으며, 처음 산란된 왕사마귀의 난괴 크기가 $40.5mm{\times}22.0mm{\times}17.3mm$로 가장 컸다. $28^{\circ}C$에서 왕사마귀의 난괴당 평균 알 수는 178.6개이었다. This study was carried out to investigate the development and oviposition characteristics of Tenodera aridifolia. The nymphs of T. aridifolia grew to adults through 6 and 7 nymphal instars. The percentage of 7 nymphal instars (7-instar type) at 25, 28, and $30^{\circ}C$ were 77.3, 76.2, and 73.5%, respectively. In the 7 instars type, total nymphal periods at 25, 28, and $30^{\circ}C$ were 71.9, 53.5, and 54.7 days, respectively. And, there was no difference in deveolpmental periods between 6- and 7-instar types at all temperatures. Egg periods were 41.3 days at $25^{\circ}C$. Adult longevity of females were 82.0 days, deposited average 3.2 oothecae at intervals of 11.7~18.7 days at $28^{\circ}C$ during her life span. The first ootheca was the largest and $40.5mm{\times}22.0mm{\times}17.3mm$ in size. The number of average eggs per oothecae was 178.6 at $28^{\circ}C$.

Distribution and Ecological Characteristics of Cocoons of the Solitary Bees Osmia cornifrons and O. pedicornis (Hymenoptera: Megachilidae)

Hyung Joo Yoon,Kyeong Yong Lee,Sun Young Kim,Yeong Mi Kim,Cheon Rak Kwon 한국양봉학회 2016 한국양봉학회 학술대회 자료집 Vol.2016 No.10

전국 사과원 주산지에서의 뿔가위벌류 사용실태 조사

이경용(Kyeong Yong Lee),윤형주(Hyung Joo Yoon),박인균(In Gyun Park),권천락(Cheon Rak Kwon),이상철(Sang Cheol Lee) 한국양봉학회 2010 韓國養蜂學會誌 Vol.25 No.1

Mason bees, Osmia spp., are used mainly to pollinate Korean and Japanese apple orchards. We surveyed the current status of mason bees to augment Osmia bees use as pollinators of fruit tree. The number of Osmia spp. produced in 2007 was 500,000. The time of mason bees supply was from 1996 to 2003 and 71.1% of apple farmers was firstly used in 2000 to 2003. The most apple farmers were a positive intention in the use of mason bees for apple pollination. The advantages in mason bees use were improvement of regular shape and seed setting percentage, which was 62.1% and 23.7%, respectively. The problems were shortage of supply and low activity at bad weather. And also, 79.1% of farmers have an intention to use mason bees for apple pollination if mason bees supply is sufficient.

Development of microsatellite markers through next-generation sequencing for bee breeding in Apis mellifera

Hye-Kyung Kim,Myeong-Lyeol Lee,Man-Young Lee,Yong-Soo Choi,Ha-Sik Shim,In Seok Kim,Cheon Rak Kwon,Hyo Yeol Chol,Sun Gwang Youn 한국양봉학회 2015 한국양봉학회 학술대회 자료집 Vol.2015 No.3

머리뿔가위벌(Osmia cornifrons)과 뿔가위벌(O. pedicornis)의 분포 및 고치의 생태적 특성

윤형주(Hyung Joo Yoon),이경용(Kyeong Yong Lee),김선영(Sun Young Kim),김영미(Yeong Mi Kim),권천락(Cheon Rak Kwon) 한국양봉학회 2016 韓國養蜂學會誌 Vol.31 No.3

Osmia cornifrons and O. pedicornis are a cavity-nesting solitary species used mainly as an apple pollinator in Korea. To elucidate the characteristics of those bees for the efficient the pollination of agricultural crops, we investigated the distribution, the number of cocoons per straw, sex ratio, and characteristics of cocoon at different locations in 2010. We collected four species of Osmia spp. consisting of O. cornifrons, O. pedicornis, O. taurus and O. satoi in Yeongwol, Bonghwa and Yechon locations. O. pedicornis was the dominant species, which accounts for 59.6% to 76.4% in the three locations. The mean number of Osmia spp. was varied in different 3 locations. In the number of cocoons per bamboo straw, there were from 7.6 to 8.5 in O. cornifrons and from 6.8 to 7.1 in O. pedicornis at one side straw, and from 16.3 to 16.5 in O. cornifrons and from 13.3 to 13.5 in O. pedicornis at both sides straw. The sex ratio of Osmia spp. were from 1:1 to 1: 2.8 in O. cornifrons and from 1:2.8 to 1:5.5 in O. pedicornis. The sex ratio of O. cornifrons and O. pedicornis was different in 3 locations. The female and male cocoons of O. cornifrons were the long elliptical-shaped and grey-brown color. The width, length and weight of the female cocoon were 5.6±0.6㎜, 10.2±0.8㎜ and 89.5±20.0㎎, respectively. The width, length and weight of the male cocoon were 5.0±0.4㎜, 9.0±0.6㎜ and 62.1±11.3㎎, respectively. The female and male cocoons of O. pedicornis were the long elliptical-shaped and polished dark-brown color. The female cocoon had the width of 6.5±0.6㎜, length of 12.9±1.0㎜ and weight of 89.5±20.0㎎. In case of the male cocoon, the width, length and weight of were 5.0±0.4㎜, 9.0±0.6㎜ and 151.5±29.4㎎, respectively. In both species of O. cornifrons and O. pedicornis, the cocoon size of the females was significantly larger than that of the male. Between those two species, the latter had a relatively bigger size than the former.

Superior triple-crossed hybrid line of honey bees, Apis mellifera L., in South Korea

Myeong-lyeol Lee,Man-young Lee,Ha-sik Sim,Yong-soo Choi,Hye-kyung Kim,In-suk Kim,Cheon-rak Kwon,Hyo-yeol Choi 한국양봉학회 2015 한국양봉학회 학술대회 자료집 Vol.2015 No.3

센서감지 및 빅데이터를 활용한 다목적기능 스마트 미러

Eun-Ji Lee,Su-Bin Noh,Hun-Geun Jo,So-Yeong Kim,Se-Hoon Cheon,Goo-Rak Kwon 한국차세대컴퓨팅학회 2021 한국차세대컴퓨팅학회 학술대회 Vol.2021 No.05

빅데이터 또는 적외선 감지 센서 등을 활용한 다양한 사물인터넷 기술은 평소 어디에서나 쉽게 접 할 수 있다. 적외선 센서를 통한 무인 출입감지 기술, 지문인식 기술을 통한 사물함 보안 시스템, 기 상 정보 및 다양한 센서를 통해 수집된 지하철 빅데이터를 기반으로 한 DB정보 시각화, 음성인식 기능을 이용한 AI서비스 제공 등의 기술을 스마트 미러라는 디스플레이 한 곳에 담아 한정적인 장 소가 아닌 다양한 상황에서 범용적으로 사용할 수 있도록 구현한다.