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- 저자 : Catia Bellucci (검색결과 1 건)
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IGF2 and IGF1R mRNAs Are Detectable in Human Spermatozoa
Rossella Cannarella,Rosita A. Condorelli,Sandro La Vignera,Catia Bellucci,Giovanni Luca,Riccardo Calafiore,Aldo E. Calogero 대한남성과학회 2020 The World Journal of Men's Health Vol.38 No.4
Purpose: Oligozoospermia is highly prevalent worldwide. Studies have reported a lower methylation rate in the H19 differentially methylated region at the sperm level in oligozoospermic patients than in controls. IGF2/H19 are the best-known pair of imprinted genes. However, no studies have yet evaluated whether they are transcribed in human sperm. To assess whether IGF2 and IGF1R mRNAs are present in human sperm and if their levels are correlated with sperm concentration and total sperm count. Materials and Methods: Sperm samples (n=22) underwent reverse-transcription quantitative polymerase chain reaction using specific primers to detect IGF2 and IGF1R mRNA levels. They were then correlated with patients’ conventional sperm parameters using the Spearman (τ) and Kendall (ρ) rank correlation coefficients. Results: Statistically significant positive correlations were found between IGF2 mRNA levels and sperm concentration (τ= 0.403, p<0.01; ρ=0.587, p<0.005) and total sperm count (τ=0.347, p<0.024; ρ=0.509, p<0.015). IGF1R mRNA levels were positively correlated with sperm concentration (τ=0.595, p<0.001; ρ=0.774, p<0.001) and total sperm count (τ=0.547, p< 0.001; ρ=0.701, p<0.001). Apart from IGF1R mRNA and sperm morphology (τ=0.325, p<0.05; ρ=0.461, p<0.05), no additional correlations were found between the levels of these transcripts and other conventional sperm parameters. Conclusions: IGF2 and IGF1R mRNAs were found to be present in human spermatozoa and their transcription levels were positively correlated with sperm concentration and total sperm count. Spermatozoa are the only source of IGF2 mRNA since IGF2 is a paternally-inherited gene. Further studies are needed to evaluate its role in human fertilization.
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