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Inactivation of Vibrio parahaemolyticus by Aqueous Ozone
( Lifang Feng ),( Kuo Zhang ),( Mengsha Gao ),( Chunwei Shi ),( Caiyun Ge ),( Daofeng Qu ),( Junli Zhu ),( Yugang Shi ),( Jianzhong Han ) 한국미생물생명공학회(구 한국산업미생물학회) 2018 Journal of microbiology and biotechnology Vol.28 No.8
Vibrio parahaemolyticus contamination causes serious foodborne illness and has become a global health problem. As a disinfectant, aqueous ozone can effectively kill a number of bacteria, viruses, parasites, and other microorganisms. In this study, three factors, namely, the aqueous ozone concentration, the exposure time, and the bacterial density, were analyzed by response surface methodology, and the aqueous ozone concentration was the most influential factor in the sterilization ratio. Under low aqueous ozone concentrations (less than 0.125 mg/l), the bacterial cell membranes remained intact, and the ozone was detoxified by intracellular antioxidant enzymes (e.g., superoxide dismutase and catalase). Under high aqueous ozone concentrations (more than 1 mg/l), cell membranes were damaged by the degree of peripheral electronegativity at the cell surface and the concentration of lactate dehydrogenase released into the extracellular space, and the ultrastructures of the cells were confirmed by transmission electron microscopy. Aqueous ozone penetrated the cells through leaking membranes, inactivated the enzymes, inhibited almost all the genes, and degraded the genetic materials of gDNA and total RNA, which eventually led to cell death.
One-step synthesis of mesoporous sulfated zirconia nanoparticles with anionic template
Hongping Pu,Yongming Luo,Liuyi Zhang,Dongquan Du,Caiyun Han,Hongyin Li,Jiangyan Li 한국화학공학회 2012 Korean Journal of Chemical Engineering Vol.29 No.10
Mesoporous sulfated zirconia nanoparticles (MSZNP) with high surface area have been synthesized by using sodium dodecyl sulfate (C12H25OSO3Na, SDS), both as a template and a sulfating agent via one-step route. On the basis of FT-IR, EDS together with NH3-TPD analyses, the SO42− anion, originated from the hydrophilic head of SDS, can be incorporated into ZrO2 to form MSZNP with super-acidity. The phase transition of ZrO2 from tetragonal to monoclinic phase was effectively inhibited by the presence of SO42−, and the formation mechanism was illustrated in detail. MSZNP(550) achieved far higher activity than CSZ(550) in transesterification of soybean oil with methanol due to the synergistic effect of strong acidity, high BET surface area and the formation of mesostructure.