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      • USE OF FLOW-THROUGH SYSTEMS TO STUDY ANAEROBIC MICROBIAL PROCESSES WITHIN COASTAL LAGOON SEDIMENT

        MARTY, Danielle,CAUMETTE, Pierre,BERTRAND, Jean Claude 영남대학교 해양과학연구소 1997 Marine Nature Vol.5 No.1

        ABSTRACTExperimental continuous flow-through systems were used to investigate the effect of continuous nutrient inputs into sediments collected from a brackish coastal lagoon. Mixed anoxic surface sediments were percolated (40 ml·h-¹) in reactors with selected substrates (sucrose, acetate or trimethylamine) that enter the anaerobic food chain at different levels. After two months of continuous flow conditions, the number and activity of fermentative, denitrifying, sulfate-reducing and methanogenic bacteria were determined. Sulfate-reduction was the dominant terminal process in natural sediment and remained so in the different reactors, exhibiting rates one to three and one to two orders of magnitude greater than denitrification and methanogenesis rates, respectively. Sulfate-reduction was limited by electron donor availability in natural sediment. Continuous supply of sucrose allowed Sulfate-reduction as high as observed when acetate was a suitable energy source for sulfate-reducers. Denitrification was markedly limited by the low availability of nitrate. Methane production was essentially derived from trimethylamine, whereas acetate and hydrogen were primarily metabolized by sulfate-reducers. Maximal Sulfate-reduction resulted in methane production and denitrification markedly reduced, and the concomitant and maximal activities of sulfate-reducers and fermenters led to the near exclusion of methanogens and denitrifiers in the mediation of carbon flow.

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