Effects of cyclosporin A treatment on the pathogenesis of avian leukosis virus subgroup J infection in broiler chickens with Marek’s disease virus exposure

김용백,Thomas P. Brown,Mary J. Pantin-Jackwood 대한수의학회 2003 Journal of Veterinary Science Vol.4 No.3

In this study, we investigated the effects of T-cell suppression on the pathogenesis of subgroup J avian leukosis virus (ALV-J). Chickens were treated with cyclosporin A (CSP) 50 mg/Kg body weight or a corresponding volume of olive oil per every three days after hatching until the end of experiment. Some of the chickens from each treatment group were infected with an isolate of ALV-J, ADOL-7501, at 2 weeks of age. The effects of viral infection were compared to uninfected birds in same treatment group. Intramuscular injection of CSP induced significant T-cell specific immunosuppression determined by decreased cutaneous basophilic hypersensitivity response and decreased lymphocyte mitogenic activity using concanavalin A. Most of the chickens examined had Marek’s disease virus infection prior to 3 weeks of age. The percentage of antibody-positive birds and antibody titers were similar in infected chickens between both treatment groups. The ratio of viremic chickens was significantly higher in CSP treated group than that of the Oil treated group. Microscopically, one CSP treated chicken had a nephroblastoma at 10 weeks post infection. At 7 and 10 weeks post-infection, more chickens had myeloid cell infiltrations in multiple organs including heart, liver and occasionally lung. Expression of ALV-J viral antigen determined by immunohistochemical staining was significantly higher in CSP treated chickens than Oil treated chickens at 10 weeks post-infection. This study indicated that chemically-induced T-cell suppression may enhance pathogenicity of the AVL-J virus in broilers.

Axial Load Testing of Hybrid Concrete Columns Consisting of UHPFRC Tube and Normal-Strength Concrete Core

Xiangguo Wu,Thomas H.-K. Kang,Issa Brown Mpalla,Chang-Soo Kim 한국콘크리트학회 2018 International Journal of Concrete Structures and M Vol.12 No.4

To investigate the axial load behavior of hybrid concrete columns consisting of an ultra-high performance fiber-reinforced concrete tube (20 mm thick, 92.6 MPa or 143.3 MPa) and normal-strength concrete core (28.2 MPa), concentric axial load tests were performed for five column specimens. The UHPFRC tube can function as a form during core-filling and as a cover having high performance at service and ultimate load conditions. Axial loading was applied to the core only, and the tube was indirectly loaded by bearing of transverse threaded bars. Test parameters included the volume fraction of steel fibers, volumetric ratio of transverse bars, and usage of wire-mesh in the tube. Test results showed that structural performance such as initial stiffness, peak load, displacement ductility, and energy absorption capacity varied greatly according to each test parameter. During the course of axial loading applied on the core only, the axial load behavior of the hybrid concrete columns generally corresponded to the full composite action at the initial loading stage and then changed to that of non-composite action (core only) after the failure of the threaded bars.

Comparison study of methane emissions from landfills with different landfill covers

Park, Soyoung,Brown, K. W.,Thomas, J. C.,Lee, In-cheol,Sung, Kijune Springer-Verlag 2010 Environmental Earth Sciences Vol.60 No.5

Development of Efficient Dynamic Aeroelasticity Model for High Fidelity Pointing Accuracy Assessment of VLBI Earth-Based Radio Antennas

Nieto Michelle Guzman,Thomas Paul V.,ElSayed Mostafa S. A.,Saad Mohamed,Brown Gary L.,Hilliard Lawrence M. 한국항공우주학회 2020 International Journal of Aeronautical and Space Sc Vol.21 No.3

Modern Earth-based radio antennas of very-long-baseline interferometry system are furnished with robust control systems for their pointing control. Their pointing accuracy is critical to the quality of the radio wave-front captured. External disturbances, particularly those of wind gusts, produce a non-negligible dynamic aeroelastic response that degrades its pointing accuracy, and yet are not mitigated by the antenna’s control system. In this paper, a high fidelity and efficient dynamic aeroelastic model of an earth-based antenna is developed which is used to study the effects of wind gusts on the antenna’s pointing accuracy. Model order reduction of the antenna structural model is carried out using Craig–Bampton method taking into consideration the dominant modal characteristics of the antenna. The aerodynamic forces are approximated using the 2D Doublet-Lattice Method. The Davenport Spectrum is used to model aerodynamic turbulences near the earth surface. The developed dynamic aeroelastic model is employed to investigate the effects of discrete and random gusts on the pointing accuracy of the antenna. It is found that the deviation in the pointing angle is more prominent in the z (azimuth) direction and it displays a quadratic dependency with respect to the mean wind speed. This behavior is attributed to the inertial component of the aeroelastic response solution represented by the gravitational field acting on the center of gravity of the main reflector and the counterweights. The developed efficient aeroelastic model can be integrated into the antenna control system for its response prediction and mitigation.

The effects of cyclophosphamide treatment on the pathogenesis of subgroup J avian leukosis virus(ALV-J)infection in broiler chickens with Marek's disease virus exposure

Yong-baek Kim,Thomas P. Brown,Mary J. Pantin-Jackwood 대한수의학회 2004 Journal of Veterinary Science Vol.5 No.1

Studies were performed to determine the effects of Bcell suppression on the pathogenesis of Subgroup J avian leukosis virus (ALV-J) in broiler chickens. Neonatal chickens were treated with cyclophosphamide (CY) or PBS, and then infected with ALV-J (ADOL-7501) at 2 weeks of age. CY treatment induced B cell specific immunosuppression throughout the experiment confirmed by decreased bursal weight, intact lymphocyte mitogenetic activity stimulated by Con A and increased relative subpopulation of CD3-positive cells as measured by flow cytometry. Chickens in this experiment had Mareks disease virus exposure prior to three weeks of age as determined by the presence of lymphocytic infiltration and antibody. Virus neutralizing antibody against ALV-J was first observed at 6 weeks post-infection in some of the infected chickens in the PBS group. As expected, none of the chickens from the CY group and uninfected chickens developed virus-neutralizing antibody. The viremic status was measured by real time RT-PCR using SYBR green I dye. The percentage of viremic chickens was significantly higher, and more chickens had high titered viremia, in the CY treated group. No neoplastic foci consistent with ALVJ infection were observed in any of the experimental chickens. The frequency and intensity of viral antigen expression determined by immunohistochemistry was significantly higher in tissues from CY treated birds than those of PBS treated chickens at 3 weeks post-infection. This study showed that B cell specific immunosuppression with CY treatment in chickens resulted in increase in viremia and viral antigen load in tissues.

Characteristics of the Clouds near the North Polar Vortex of Saturn Observed by Cassini/VIMS

Joo-Hyeon Kim,Kevin H. Baines,Thomas W. Momary,Bonnie J. Buratti,Robert H. Brown,Roger N. Clark,Phillip D. Nicholson,Cassini/VIMS Science Team 한국천문학회 2009 天文學會報 Vol.34 No.1

Utilization of cryopreserved sperm cells based on the indication for storage

Graham Luke Machen,Stephanie E. Harris,Erin T. Bird,Monica L. Brown,Dale A. Ingalsbe,Milaida M. East,Michelle Reyes,Thomas J. Kuehl 대한비뇨의학회 2018 Investigative and Clinical Urology Vol.59 No.3

Purpose: Cryopreservation of sperm from human semen has been available since the 1950s. The actual utilization of available cryopreservation technology has been infrequently reported. We set out to examine the utilization and outcomes of cryopreserved sperm cells based on the indication for storage. Materials and Methods: A dataset was developed from retrospective review. The purposes for cryopreservation, eventual utilization, and outcomes of use for insemination were recorded. The types of utilization were accumulated as proportions for different purposes. The timing for use of samples for insemination procedures was evaluated using survival statistics. The frequency of patients arranging to destroy samples was also reported. Results: From September 1988 through March 2015, 1442 samples were cryopreserved. Samples were cryopreserved for four primary purposes: infertility treatments focused on intrauterine insemination (IUI) or in vitro fertilization/intracellular injection (IVF/ICSI), for fertility preservation related to cancer treatment, or prior to military deployment. Total utilization rates were 19.3%. Samples cryopreserved for IUI were more likely to be used (64.3%), while samples cryopreserved as backup for IVF/ICSI were more likely to be destroyed (29.8%). Pregnancy rates varied based on the indication and ART used. Pregnancies per cycle were 35% for IVF/ICSI and were 10% for IUI. Conclusions: Cryopreservation of sperm is a valuable and underutilized resource, particularly amongst male cancer patients. This technology can facilitate infertility treatments based on a variety of indications, including deployment—a patient cohort unique to our dataset.

Meeting Report: Translational Advances in Cancer Prevention Agent Development Meeting

Mark Steven Miller,Peter J. Allen,Powel H. Brown,Andrew T. Chan,Margie L. Clapper,Roderick H. Dashwood,Shadmehr Demehri,Mary L. Disis,Raymond N. DuBois,Robert J. Glynn,Thomas W. Kensler,Seema A. Khan 대한암예방학회 2021 Journal of cancer prevention Vol.26 No.1

The Division of Cancer Prevention of the National Cancer Institute (NCI) and the Office of Disease Prevention of the National Institutes of Health co-sponsored the Translational Advances in Cancer Prevention Agent Development Meeting on August 27 to 28, 2020. The goals of this meeting were to foster the exchange of ideas and stimulate new collaborative interactions among leading cancer prevention researchers from basic and clinical research; highlight new and emerging trends in immunoprevention and chemoprevention as well as new information from clinical trials; and provide information to the extramural research community on the significant resources available from the NCI to promote prevention agent development and rapid translation to clinical trials. The meeting included two plenary talks and five sessions covering the range from pre-clinical studies with chemo/immunopreventive agents to ongoing cancer prevention clinical trials. In addition, two NCI informational sessions describing contract resources for the preclinical agent development and cooperative grants for the Cancer Prevention Clinical Trials Network were also presented.

Low versus standard central venous pressure during laparoscopic liver resection: A systematic review, meta-analysis and trial sequential analysis

Mina Stephanos,Christopher M. B. Stewart,Ameen Mahmood,Christopher Brown,Shahin Hajibandeh,Shahab Hajibandeh,Thomas Satyadas 한국간담췌외과학회 2024 Annals of hepato-biliary-pancreatic surgery Vol.28 No.2

To compare the outcomes of low central venous pressure (CVP) to standard CVP during laparoscopic liver resection. The study design was a systematic review following the PRISMA statement standards. The available literature was searched to identify all studies comparing low CVP with standard CVP in patients undergoing laparoscopic liver resection. The outcomes included intraoperative blood loss (primary outcome), need for blood transfusion, mean arterial pressure, operative time, Pringle time, and total complications. Random- effects modelling was applied for analyses. Type I and type II errors were assessed by trial sequential analysis (TSA). A total of 8 studies including 682 patients were included (low CVP group, 342; standard CVP group, 340). Low CVP reduced intraoperative blood loss during laparoscopic liver resection (mean difference [MD], –193.49 mL; 95% confidence interval [CI], –339.86 to –47.12; p = 0.01). However, low CVP did not have any effect on blood transfusion requirement (odds ratio [OR], 0.54; 95% CI, 0.28–1.03; p = 0.06), mean arterial pressure (MD, –1.55 mm Hg; 95% CI, –3.85–0.75; p = 0.19), Pringle time (MD, –0.99 minutes; 95% CI, –5.82–3.84; p = 0.69), operative time (MD, –16.38 minutes; 95% CI, –36.68–3.39; p = 0.11), or total complications (OR, 1.92; 95% CI, 0.97–3.80; p = 0.06). TSA suggested that the meta-analysis for the primary outcome was not subject to type I or II errors. Low CVP may reduce intraoperative blood loss during laparoscopic liver resection (moderate certainty); however, this may not translate into shorter operative time, shorter Pringle time, or less need for blood transfusion. Randomized controlled trials with larger sample sizes will provide more robust evidence.

Titan at 3 microns: Newly identified spectral features and an improved analysis of haze opacity

Seo, Haingja,Kim, Sang Joon,Kim, Joo Hyeon,Geballe, Thomas R.,Courtin, Ré,gis,Brown, Linda R. Elsevier 2009 Icarus Vol.199 No.2

<P><B>Abstract</B></P><P>We have reanalyzed the high-resolution spectrum of Titan between 2.87 and 3.12 μm observed with NIRSPEC/Keck II on 2001 Nov. 21 in southern summer, using updated CH<SUB>3</SUB>D and C<SUB>2</SUB>H<SUB>6</SUB> line-by-line models. From new synthetic spectra, we identify all but a few of the previously unidentified significant absorption spectral features in this wavelength range as due to these two species, both of which had been previously detected by Voyager and ground-based observations at other wavelengths. We also derive opacities and reflectivities of haze particles as functions of altitude for the 2.87–2.92 μm wavelength range, where Titan's atmosphere is partially transparent down to the surface. The extinction per unit altitude is observed to increase from 100 km (∼8 mbar) toward lower altitude. The derived total optical depth is approximately 1.1 for the 2.87–2.92 μm range. At wavelengths increasing beyond 2.92 μm the haze layers become much more optically thick, and the surface is rapidly hidden from view. These conclusions apply to equatorial and southern-temperate regions on Titan, excluding polar regions. We also find it unlikely that there is a large enhancement of the tropospheric CH<SUB>4</SUB> mole fraction over the value reported from analysis of the Huygens/GCMS observations.</P>