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- 저자 : Bonggyu Min (검색결과 5 건)
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김봉규(Bonggyu Kim),이성민(Seong-min Lee),김영근(Y. G. KIM) 한국자동차공학회 2007 한국자동차공학회 춘 추계 학술대회 논문집 Vol.- No.-
Natural gas has been reported to have good potential for alternative vehicle fuel due to its economical and clean characteristics. Thus, we converted decrepit IDI Diesel engine into a dedicated natural gas engine to reduce the exhaust emissions in a retrofit of a diesel-fueled vehicle, and got a certificate of quality and emission form MOE(Ministry of Environment Republic of Korea). The objective of this paper is to clarify the combustion characteristics and emissions of prechamber type small class CNG engine. This paper presents combustion characteristics obtained from running a 2.5L, 4-cylinder CNG engine retrofitted IDI diesel engine with engine dynamometer. BSFC, emissions, fuel consumption and combustion pressure were measured under steady state operating conditions especially at partial load for CNG engine. From the experimental results, we found out that combustion characteristics of prechamber type CNG engine is quite different from that of normal CNG engine especially at low load - low speed condition.
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김병용,Bonggyu Min,Yoo-Jin Kwon,SUNG-YURB PARK,Jong Hyun Lim,Chang-Hun Shin,Byoung Kook Kim 건강기능식품미래포럼 2022 건강기능식품미래포럼 학술지 Vol.2 No.4
Lactiplantibacillus plantarum CKDB008 was isolated from a Korean fermented food, kimchi. In order to investigate its potential as a probiotic in the food industry, the genome sequence of L. plantarum CKDB008 was analyzed to confirm its probiotic properties and safety at the genome level. The CKDB008 strain has one circular chromosome of 3.2 Mbp, with 44.6% guanine-cytosine content. A comparative genomic analysis confirmed that its genome was similar to those of 19 L. plantarum strains used as probiotics. Pan-genome analysis of 20 L. plantarum strains identified 6,236 genes, including 2,006 core, 255 soft core, 1,485 shells, and 2,490 cloud genes. These results reveal the ‘open’ nature of L. plantarum pan-genome. Among the predicted 3,129 protein coding genes, the genome contains genes associated with important characteristics of probiotics such as protease system, amino acid biosynthesis, major carbohydrate-active enzymes, stress resistance, and cell adhesion. In addition, genes encoding bacteriocins, immunoregulatory, and gamma-aminobutyric acid biosynthesis were also identified, but safety-related genes related to antibiotic resistance and virulence factors were not. The results obtained strongly suggest that CKDB008 strain is genetically safe and has the desired properties required for a probiotic as well as support that L. plantarum CKDB008 strain has a potential as a probiotic that can be used for the industrial purpose.
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Kim Myung-Soo,Min Bonggyu,Kim Insu,Kwon Yoo Jin,Park Sung Yurb,Kim Byung-Yong,Kim Byoung Kook,박성선 건강기능식품미래포럼 2021 건강기능식품미래포럼 학술지 Vol.1 No.3
Streptococcus thermophilus is a bacterial species widely used in the food industry for producing dairy fermented foods as well as probiotics. In this study, the whole genome of the S. thermophilus CKDB027 strain used in probiotic products was sequenced, and the genetic features related to its safety and functionality were determined. The CKDB027 strain has a single circular chromosome of approximately 1.88 Mb with 39.0% GC content. The genome study has shown the absence of antibiotic resistance and virulence related genes. To identify the unique genetic features of CKDB027, genomic comparisons with other 18 S. thermophilus used in probiotic industry were conducted. The results showed that the CKDB027 strain shared a common ancestor with several strains isolated from milk or yogurt. The CKDB027 strain possesses genes related to lactose catabolism, proteolysis, stress resistance, defense system, and adherence that are major function of probiotics. In addition, a gene cluster producing exopolysaccharides was detected. These findings indicate that the S. thermophilus CKDB027 strain is genetically safe and has beneficial genetic features for human health when it is used in dairy industry as a health functional food.
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Jang You Jin,Min Bonggyu,Lim Jong Hyun,Kim Byung-Yong 한국미생물·생명공학회 2023 Journal of microbiology and biotechnology Vol.33 No.9
Changes in the gut microbiome cause recolonization by pathogens and inflammatory responses, leading to the development of intestinal disorders. Probiotics administration has been proposed for many years to reverse the intestinal dysbiosis and to enhance intestinal health. This study aimed to evaluate the inhibitory effects of two newly designed probiotic mixtures, Consti-Biome and SensiBiome, on two enteric pathogens Staphylococcus aureus and Escherichia coli that may cause intestinal disorders. Additionally, the study was designed to evaluate whether Consti-Biome and Sensi-Biome could modulate the immune response, produce short-chain fatty acids (SCFAs), and reduce gas production. Consti-Biome and Sensi-Biome showed superior adhesion ratios to HT-29 cells and competitively suppressed pathogen adhesion. Moreover, the probiotic mixtures decreased the levels of pro-inflammatory cytokines, such as tumor necrosis factor-α, interleukin (IL)-6 and IL1β. Cell-free supernatants (CFSs) were used to investigate the inhibitory effects of metabolites on growth and biofilms of pathogens. Consti-Biome and Sensi-Biome CFSs exhibited antimicrobial and anti-biofilm activity, where microscopic analysis confirmed an increase in the number of dead cells and the structural disruption of pathogens. Gas chromatographic analysis of the CFSs revealed their ability to produce SCFAs, including acetic, propionic, and butyric acid. SCFA secretion by probiotics may demonstrate their potential activities against pathogens and gut inflammation. In terms of intestinal symptoms regarding abdominal bloating and discomfort, Consti-Biome and Sensi-Biome also inhibited gas production. Thus, these two probiotic mixtures have great potential to be developed as dietary supplements to alleviate the intestinal disorders.
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