Assessment of physical stability and photoprotective activity of topical formulations added with calendula oil

Mishra, Arun K.,Mishra, Amrita,Chattopadhyay, Pronobesh 경희한의학연구센터 2012 Oriental Pharmacy and Experimental Medicine Vol.12 No.1

In the present work, the changes on physical properties (pH, viscosity, flow index and tixotropy) of topical formulations were evaluated following addition of calendula oil containing flavonoids. Also the photoprotective effect of these topical formulations were evaluated against ultra violet rays (UVR) induced sunburn in terms of sun protection factor (SPF). Formulation added with sun flower oil was used to compare the physical stability and photoprotective activity. Formulations added with calendula oil exhibited an average pH 7.2 and pseudoplastic behavior. The tixotropy values for formulations F3 and F4, after addition with calendula oil were found to be statistically decreased when compared with FS and Fb. The rheological parameters were constant during the study and the rheograms exhibited no sign of instability when studied at $4^{\circ}$, $27^{\circ}$ and $40^{\circ}C$ on different days of storage. The F3 and F4 samples showed statistically higher SPF (14 and 15 respectively) when compared with Fb (without active sunscreening agent). The highest SPF value 16 was recorded for FS (control formulation added with sun flower oil) while Fb showed lowest SPF vale 5.We concluded that F3, F4 and FS showed higher SPF and physical stability. This study of physical stability and photoprotectice activity of topical formulation can help in the development of sunscreen formulation.

Quantification of the secondary metabolites by HPTLC, analgesic and antipyretic activity evaluation of Ficus racemosa L. leaves

Amit Kumar,Amrita Mishra,Arun Kumar Mishra,Harpreet Singh 경희대학교 융합한의과학연구소 2019 Oriental Pharmacy and Experimental Medicine Vol.19 No.1

There have been no reports of simultaneous quantification of kaempferol and quercetin in Ficus racemosa L. Objective of the present work included to perform extraction using petroleum ether (PEE) and ethanol (EE), to perform preliminary phytochemical test, quantitative estimation of phytochemicals and simultaneous estimation of kaempferol and quercetin in Ficus racemosa L. using high performance thin layer chromatography (HPTLC) method and to assess the analgesic and antipyretic activity of the extracts. PEE and EE obtained from cold maceration technique. Extracts were subjected to qualitative test and estimated quantitatively. Extracts were applied on silica gel G 60 F254 plate; solvent-Toluene: Chloroform: Acetone 4.5:4.5:1 (v/v) and scanned at 254 nm. The extracts were also subjected to analgesic activity (hot plate method and tail immersion method) and antipyretic (Brewer’s yeast induced pyrexia model and Lipopolysaccharide induced pyrexia mode) activity. Findings of preliminary phytochemical test and quantitative estimation of phytochemicals confirms the presence of flavonoids in both the extracts (PEE and EE). The amount of flavonoids in PEE and EE were found to be 24.58 ± 0.60 mg/100 g and 21.01 ± 0.58 mg/100 g respectively. HPTLC system resulted well resolved bands for quercetin (Rf 0.38) and kaempferol (Rf 0.55). The validated HPTLC method was found suitable for kaempferol and quercetin quantification in Ficus racemosa L. Hot plate reaction time response was improved in a dose-dependent manner in a group of rats treated with PEE suggesting its analgesic potential. In view of analgesic potential by tail immersion method, PEE (400 mg/kg b.w.) showed no significant difference in tail flick reaction time as shown by standard treated animal (Aspirin 100 mg/kg b.w.). The findings of antipyretic activity suggested that PEE (400 mg/kg b.w.) have no significant difference in treating pyrexia when compared with Paracetamol (100 mg/kg). PEE (400 mg/kg b.w.) was found to be most potent. It may be due to the presence of comparatively high amount of flavonoids in PEE confirmed by the preliminary phytochemical test, quantitative estimation of phytochemicals and HPTLC quantification the PEE was found to be more effective when compared to EE.

Antibacterial activity of ZnO nanoparticles prepared via non-hydrolytic solution route

Wahab, Rizwan,Mishra, Amrita,Yun, Soon-Il,Kim, Young-Soon,Shin, Hyung-Shik Springer-Verlag 2010 Applied microbiology and biotechnology Vol.87 No.5

An up-to-date review on chemistry and biological activities of Senna occidentalis (L.) Link Family: Leguminosae

Singh Harpreet,Chahal Piyush,Mishra Amrita,Mishra Arun Kumar 경희대학교 융합한의과학연구소 2020 Oriental Pharmacy and Experimental Medicine Vol.20 No.3

Senna occidentalis (L.) Link Family: Leguminosae, one of the important Ayurvedic plants, to treat various ailments. The current review embarks on the detected secondary metabolites, biological properties and toxicological aspects of extracts of S. occidentalis (L.) Link. With an aim to encourage the recent and advanced research on extracts, the present review is presented. The traditional medicinal importance of S. occidentalis (L.) Link has been identified in tropical and subtropical regions to treat various ailments. All the available informations on S. occidentalis (L.) Link was collected via electronic search (using Pubmed, SciFinder, Scirus, Google Scholar, Agricola and Web of Science) and literature study done at library. About 38 phytocompounds have been isolated from S. occidentalis (L.) Link; the most important includes anthraquinones, flavonoid glycosides, xanthones, tetrahydro anthracenes, terpenes and phytosterol etc. In vivo biological studies of the extracts show that S. occidentalis (L.) Link possesses analgesic, anthelmintic, antidiabetic, anticonvulsant, antitrypanosomal, antioxidant, hepatoprotective, myeloprotective, wound healing, anticancer, anti-inflammatory and larvicidal activity. The extracts need to be further investigated to describe the extent of the biological effects, most advantageous dosage required, and mechanisms of action behind biological effects, safety profile and probable side effects. Moreover, clinical studies are required to support the curative potential of S. occidentalis (L.) Link.

Percolation bacterial leaching of low-grade chalcopyrite using acidophilic microorganisms

Karanam Srinivasa Rao,Amrita Mishra,Devbrata Pradhan,Gautam Roy Chaudhury,Birendra Kumar Mohapatra,Trupti Das,Lala Bihari Sukla,Barada Kanta Mishra 한국화학공학회 2008 Korean Journal of Chemical Engineering Vol.25 No.3

Bioleaching studies were carried out in percolation columns using low-grade copper containing rock (granite). The lixiviant consisted of acidified ferric sulfate containing acidophilic microorganisms. The iron oxidizing strain was isolated from Malanjkhand mine water and after adaptation the iron oxidation observed to be 500 mg/L/h. Leaching parameters studied were lixiviant flow rate, particle size and bed height. It was observed that leaching efficiency increased with decrease of particle size and lixiviant flow rate. The precipitation of iron during leaching observed to be low as pH was maintained at 2. Based on the leaching kinetics, a unified rate equation was developed and shown as ra(dp)[−0.61 (F)−0.76 (Hb)0.97.]수식

Formation of ZnO Micro-Flowers Prepared via Solution Process and their Antibacterial Activity

Wahab, Rizwan,Kim, Young-Soon,Mishra, Amrita,Yun, Soon-Il,Shin, Hyung-Shik Springer 2010 NANOSCALE RESEARCH LETTERS Vol.5 No.10

<P>This paper presents the fabrication and characterization of zinc oxide micro-flowers and their antibacterial activity. The micro-flowers of zinc oxide composed of hexagonal nanorods have been prepared via solution process using precursor zinc acetate di-hydrate and sodium hydroxide in 3 h of refluxing time at ~90°C. The antibacterial activities of grown micro-flowers were investigated against four pathogenic bacteria namely <I>S. aureus, E. coli, S. typhimurium and K. pneumoniae</I> by taking five different concentrations (5–45 μg/ml) of ZnO micro-flowers (ZnO-MFs). Our investigation reveals that at lowest concentration of ZnO-MFs solution inhibiting the growth of microbial strain which was found to be 5 μg/ml for all the tested pathogens. Additionally, on the basis of morphological and chemical observations, a chemical reaction mechanism of ZnO-MFs composed of hexagonal nanorods was also proposed.</P>

Management of patients with allergy to local anesthetics: two case reports

Arya, Varun,Arora, Geetanjali,Kumar, Sanjeev,Kaur, Amrita,Mishra, Santosh The Korean Dental Society of Anesthsiology 2021 Journal of Dental Anesthesia and Pain Medicine Vol.21 No.6

Lidocaine is the most commonly used local anesthetic (LA) agent in various dental as well as oral and maxillofacial procedures. Although rare, adverse effects and allergic reactions to lidocaine have been reported. In patients with suspected allergy to LA or a history of such reaction, careful history-taking and allergy testing should be performed to choose an alternative LA agent to avoid any adverse effects. Here, we present two cases of delayed hypersensitivity reaction to lidocaine, wherein the patients presented with erythema, edema, and itching. Intradermal testing confirmed allergic reaction to lidocaine, and the patients underwent successful dental treatment using an alternative LA agent. This report highlights the importance of allergy testing prior to LA use considering the serious consequences of allergy to these agents and describes the management of such patients using an alternative LA agent.