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RISS 인기검색어
- 검색키워드
- 저자 : Allen Y. Yi (검색결과 2 건)
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High-throughput 3D spheroid culture and drug testing using a 384 hanging drop array
Tung, Yi-Chung,Hsiao, Amy Y.,Allen, Steven G.,Torisawa, Yu-suke,Ho, Mitchell,Takayama, Shuichi Royal Society of Chemistry 2011 The Analyst Vol.136 No.3
<P>Culture of cells as three-dimensional (3D) aggregates can enhance <I>in vitro</I> tests for basic biological research as well as for therapeutics development. Such 3D culture models, however, are often more complicated, cumbersome, and expensive than two-dimensional (2D) cultures. This paper describes a 384-well format hanging drop culture plate that makes spheroid formation, culture, and subsequent drug testing on the obtained 3D cellular constructs as straightforward to perform and adapt to existing high-throughput screening (HTS) instruments as conventional 2D cultures. Using this platform, we show that drugs with different modes of action produce distinct responses in the physiological 3D cell spheroids compared to conventional 2D cell monolayers. Specifically, the anticancer drug 5-fluorouracil (5-FU) has higher anti-proliferative effects on 2D cultures whereas the hypoxia activated drug commonly referred to as tirapazamine (TPZ) are more effective against 3D cultures. The multiplexed 3D hanging drop culture and testing plate provides an efficient way to obtain biological insights that are often lost in 2D platforms.</P> <P>Graphic Abstract</P><P>A 384-well format hanging drop culture plate that enables spheroid formation, culture, and drug testing using existing high-throughput screening instruments. <IMG SRC='http://pubs.rsc.org/services/images/RSCpubs.ePlatform.Service.FreeContent.ImageService.svc/ImageService/image/GA?id=c0an00609b'> </P>
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Xiongyu Luo,Muxuan Yang,Jinyu Bu,Tiantong Chen,Allen Y. Yi,Weinan Xu 한국고분자학회 2023 Macromolecular Research Vol.31 No.8
The integration of two or more types of metal oxides in the same nanostructures leads to several unique characteristics including tunable optical properties and band gap, enhanced catalytic property and stability. However, current approaches for the generation of those mixed metal oxides are predominantly based on bottom-up synthesis, which have limitations in fabricating precise nanostructures with monodispersed size and shape, and low compatibility with lithography-based micro-/nanofabrication processes. Here, we report a new approach for the precise fabrication of nanostructured mixed metal oxides (NMMOs) by the integration of soft nanoimprinting and sol–gel synthesis. The hybrid ink for nanoimprinting is composed of metal–organic compound and metal oxide colloidal nanoparticles of different metal species, after nanoimprinting, sol–gel reaction, and calcination, NMMOs with well-defined size and geometry are successfully obtained. The morphology, size, composition, and crystalline structure of the NMMOs are systematically tuned and studied, their potential application in photocatalysis is also investigated.
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