Histopathological and genetic changes proved the anti‑cancer potential of free and nano‑capsulated sinapic acid

Doaa A. Badr,Mohamed E. Amer,Wagih M. Abd‑Elhay,Mohamed S. M. Nasr,Tamer M. M. Abuamara,Harbi Ali,Aly F. Mohamed,Maha A. Youssef,Nasser S. Awwad,Yi‑Hsu Ju,Ahmed E. Fazary 한국응용생명화학회 2019 Applied Biological Chemistry (Appl Biol Chem) Vol.62 No.5

Cancer is known to be a fierce disease that causes a large percentage of the deaths worldwide. The common cancer treatments; chemotherapy, radiotherapy and surgery are known for their severe side effects; therefore scientists are working on finding solutions to reduce these drawbacks. One of these treatment systems is the sustained released drugs formulations, these systems depend on the encapsulation of the chemotherapy within an emulsifying agent, in order to obtain a slow drug release of low doses over long time intervals. In this study, the anti-cancer effects of free and encapsulated sinapic acid was tested against lung (A549), and colon (CaCo2) cancer cell lines, along with normal fibroblast cells (HFB4) as a negative control. MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay was performed for IC50 evaluation, also cell cycle assay was performed to detect cell cycle arrest status and related anti-apoptotic and pro-apoptotic; Blc-2, BAX, and P53 gene profile fold changes post cellular treatment. Data recorded revealed that encapsulated SA showed a lower toxicity than the free form to both cell lines and also to the normal cells. The cell cycle analysis showed a cell cycle arrest at the G2/M phase post cell treatment with the free and encapsulated sinapic acid accompanied with up regulation of Bax and P53 and a down regulation of Blc-2 genes in both cell lines. The data suggest a promising anti-cancer and anti-proliferative potential of free and encapsulated sinapic acid. Also they show that the anti-cancer effect of free and encapsulated sinapic acid is quite close.

New ursane triterpenoids from Ficus pandurata and their binding affinity for human cannabinoid and opioid receptors

Amgad I. M. Khedr,Sabrin R. M. Ibrahim,Gamal A. Mohamed,Hany E. A. Ahmed,Amany S. Ahmad,Mahmoud A. Ramadan,Atef E. Abd El-Baky,Koji Yamada,Samir A. Ross 대한약학회 2016 Archives of Pharmacal Research Vol.39 No.7

Phytochemical investigation of Ficus pandurataHance (Moraceae) fruits has led to the isolation of two newtriterpenoids, ficupanduratin A [1b-hydroxy-3b-acetoxy-11a-methoxy-urs-12-ene] (11) and ficupanduratin B [21ahydroxy-3b-acetoxy-11a-methoxy-urs-12-ene] (17), alongwith 20 known compounds: a-amyrin acetate (1), a-amyrin(2), 3b-acetoxy-20-taraxasten-22-one (3), 3b-acetoxy-11amethoxy-olean-12-ene (4), 3b-acetoxy-11a-methoxy-12-ursene (5), 11-oxo-a-amyrin acetate (6), 11-oxo-b-amyrinacetate (7), palmitic acid (8), stigmast-4,22-diene-3,6-dione(9), stigmast-4-ene-3,6-dione (10), stigmasterol (12), b-sitosterol(13), stigmast-22-ene-3,6-dione (14), stigmastane-3,6-dione (15), 3b,21b-dihydroxy-11a-methoxy-olean-12-ene (16), 3b-hydroxy-11a-methoxyurs-12-ene (18), 6-hydroxystigmast-4,22-diene-3-one (19), 6-hydroxystigmast-4-ene-3-one (20), 11a,21a-dihydroxy-3b-acetoxy-urs-12-ene(21), and b-sitosterol-3-O-b-D-glucopyranoside (22). Compound21 is reported for the first time from a natural source. The structures of the 20 compounds were elucidated on thebasis of IR, 1D (1H and 13C), 2D (1H–1H COSY, HSQC,HMBC and NOESY) NMR and MS spectroscopic data, inaddition to comparison with literature data. The isolatedcompounds were evaluated for their anti-microbial, antimalarial,anti-leishmanial, and cytotoxic activities. In addition,their radioligand displacement affinity on opioid andcannabinoid receptors was assessed. Compounds 4, 11, and15 exhibited good affinity towards the CB2 receptor, withdisplacement values of 69.7, 62.5 and 86.5 %, respectively. Furthermore, the binding mode of the active compounds inthe active site of the CB2 cannabinoid receptors was investigatedthrough molecular modelling.

Secreted Phosphoprotein 1 Promoter Genetic Variants Are Associated with the Response to Pegylated Interferon α Plus Ribavirin Combination Therapy in Egyptian Patients with Chronic Hepatitis C Virus Infection

( Fahmy T Ali ),( Mohamed A M Ali ),( Mayada M A Elgizawy ),( Ahmed M Elsawy ) 대한소화기학회 2015 Gut and Liver Vol.9 No.4

Background/Aims: The T-helper 1 (TH1) immune reaction is essential for the eradication of hepatitis C virus (HCV) during pegylated interferon α (PEG-IFN-α)- and ribavirin (RBV)-based therapy in chronic HCV patients. Secreted phosphoprotein 1 (SPP1) was shown to be a crucial cytokine for the initiation of a TH1 immune response. We aimed to investigate whether SPP1 single nucleotide polymorphisms (SNPs) may influence sustained virological response (SVR) rates. Methods: Two SNPs in the promoter region of SPP1 at the .443 C>T and .1748 G>A loci were genotyped in 100 patients with chronic HCV genotype 4 infection using a TaqMan SNP genotyping assay. Results: Sixty-seven patients achieved a SVR, and 33 patients showed no SVR. Patients carrying the T/T genotype at the .443 locus showed a significantly higher SVR rate than those carrying the C/T or C/C genotype (83.67% vs 50.98%, p<0.001). At the .1748 locus, the SVR rate was significantly higher in patients with the G/G genotype than in those with the A/A genotype (88.89% vs 52.63%, p=0.028) and in patients with the G/A genotype than in those with the A/A genotype (85.29% vs 52.63%, p=0.001). Conclusions: SPP1 SNPs at .443 C>T and .1748 G>A loci may be useful markers for predicting the response to PEG-IFN-α-2b plus RBV therapy in Egyptian patients with chronic HCV genotype 4 infection. (Gut Liver 2015;9:516-524)

Stem cell-derived exosomes for dentin-pulp complex regeneration: a mini-review

Hammouda Dina A.,Mansour Alaa M,Saeed Mahmoud A.,Zaher Ahmed R.,Grawish Mohammed E. 대한치과보존학회 2023 Restorative Dentistry & Endodontics Vol.48 No.2

This mini-review was conducted to present an overview of the use of exosomes in regenerating the dentin-pulp complex (DPC). The PubMed and Scopus databases were searched for relevant articles published between January 1, 2013 and January 1, 2023. The findings of basic in vitro studies indicated that exosomes enhance the proliferation and migration of mesenchymal cells, as human dental pulp stem cells, via mitogen-activated protein kinases and Wingless-Int signaling pathways. In addition, they possess proangiogenic potential and contribute to neovascularization and capillary tube formation by promoting endothelial cell proliferation and migration of human umbilical vein endothelial cells. Likewise, they regulate the migration and differentiation of Schwann cells, facilitate the conversion of M1 pro-inflammatory macrophages to M2 anti-inflammatory phenotypes, and mediate immune suppression as they promote regulatory T cell conversion. Basic in vivo studies have indicated that exosomes triggered the regeneration of dentin-pulp–like tissue, and exosomes isolated under odontogenic circumstances are particularly strong inducers of tissue regeneration and stem cell differentiation. Exosomes are a promising regenerative tool for DPC in cases of small pulp exposure or for whole-pulp tissue regeneration.

Optimization Driven MapReduce Framework for Indexing and Retrieval of Big Data

( Hemn Barzan Abdalla ),( Awder Mohammed Ahmed ),( M. A. Al Sibahee ) 한국인터넷정보학회 2020 KSII Transactions on Internet and Information Syst Vol.14 No.5

With the technical advances, the amount of big data is increasing day-by-day such that the traditional software tools face a burden in handling them. Additionally, the presence of the imbalance data in big data is a massive concern to the research industry. In order to assure the effective management of big data and to deal with the imbalanced data, this paper proposes a new indexing algorithm for retrieving big data in the MapReduce framework. In mappers, the data clustering is done based on the Sparse Fuzzy-c-means (Sparse FCM) algorithm. The reducer combines the clusters generated by the mapper and again performs data clustering with the Sparse FCM algorithm. The two-level query matching is performed for determining the requested data. The first level query matching is performed for determining the cluster, and the second level query matching is done for accessing the requested data. The ranking of data is performed using the proposed Monarch chaotic whale optimization algorithm (M-CWOA), which is designed by combining Monarch butterfly optimization (MBO) [22] and chaotic whale optimization algorithm (CWOA) [21]. Here, the Parametric Enabled-Similarity Measure (PESM) is adapted for matching the similarities between two datasets. The proposed M-CWOA outperformed other methods with maximal precision of 0.9237, recall of 0.9371, F1-score of 0.9223, respectively.

Synergistic activity of Bacillus thuringiensis δ‐endotoxin and TMOF against Culex pipiens and Spodoptera littoralis larvae

Ahmed M.A. Mohammed,Mervat R. DIAB,Sayed M.S. KHALIL 한국곤충학회 2018 Entomological Research Vol.48 No.3

Trypsin Modulating Oostatic Factor (TMOF) is a decapeptide hormone that inhibits the biosynthesis of digestive enzymes in the mosquito midgut. The hormone inhibits food digestion and ultimately leads to starvation and death. It has been used as a biological insecticide to control mosquitoes. In an attempt to increase the insecticidal activity of TMOF, a combination of CryIC (δ‐endotoxin from Bacillus thuringiensis) and TMOF was determined. Eight recombinant proteins fused with GST (glutathione‐S‐transferase) were expressed in Escherichia coli cells. Their insecticidal activities were determined against Culex pipiens and Spodoptera littoralis larvae. Purified GST‐TMOF and its analogue GST‐YDPAS exhibited a moderate toxicity on C. pipiens larvae with LC50 of 145.9 and 339.9 μg/mL, respectively. Unexpectedly, no mortality was observed in first instar larvae of S. littoralis. Puirified GST‐TMOF and GST‐YDPAS together with Bt toxin showed a synergistic toxic effect on both Culex and Spodoptera larvae. In the presence of 100 μg/mL GST‐TMOF and GST‐YDPAS, the median lethal concentration of entomocidus on culex larvae decreased from 52.1 to 16.7 and 31.9 μg/mL, respectively. Likewise, GST‐TMOF and GST‐YDPAS incorporated with 0.07 μg/cm2 of enotmocidus showed insecticidal activity against S. littoralis with LC50 of 16.4 and 21.9 μg/cm2. The E. coli lysates containing GST‐CryIC and its 3′‐truncated version showed low toxicity against the lepidopteran insect (10.8 and 16.6 μg/cm2) compared to 0.15 μg/cm2 of the native crystalline form of CryIC. Similarly, the mosquitocidal activity of the recombinant Bt toxins was low.

A target‐specific feeding toxicity of <i>β</i>₁ integrin dsRNA against diamondback moth, Plutella xylostella

Mohamed, Ahmed A. M.,Kim, Yonggyun Wiley Subscription Services, Inc., A Wiley Company 2011 Archives of Insect Biochemistry and Physiology Vol.78 No.4

<P>Integrin is a cell-surface protein consisting of a and beta heterodimers. A predicted amino acid sequence of an integrin subunit of the diamondback moth, Plutella xylostella, was highly homologous to other lepidopteran beta 1 subunits and possessed essential functional domains. The beta 1 integrin of P. xylostella (beta Px1) was expressed in all developmental stages of P. xylostella. It was also expressed in all tested tissues including hemocyte, fat body, gut, and epidermis of last instar. When beta Px1 expression was suppressed by injection of dsRNA specific to beta Px1 (dsRNA beta Px1), the treated larvae exhibited significant suppression in immune response and also suffered significant larval mortality. When dsRNA beta Px1 was orally fed to young larvae, it suppressed the expression of aPx1 and resulted in a significant mortality. By contrast, a dsRNA specific to beta 1 subunit of Spodoptera exigua gave little adverse effects on beta Px1 expression and larval development when it was treated by injection or oral administration, though these two genes showed 71 parts per thousand sequence homology. These results suggest a target-specific RNA interference of dsRNA beta Px1, which causes significant mortality to P. xylostella by feeding treatment. (c) 2011 Wiley Periodicals, Inc.</P>

Modifying the aggregated wind farm model with a controller

Badr, Mohammed. A.,Atallah, Ahmed. M.,Bayoumi, Mona A. Techno-Press 2015 Advances in energy research Vol.3 No.3

A large wind farm can be simplified by the aggregated wind farm models for load flow, steady and transient stability studies. When a fault (such as a short circuit) happens in a large wind farm, some of wind turbines trip while others do not. This paper is to design a controller to modify the aggregated wind farm model in the case of one or more unit removed or added from the complete model. This is without stopping the simulation process during performing the steady state and transient analysis of the whole system. This controller can modulate the status of the wind turbines in the aggregated model in a given farm according to any change in this farm. By this controller, we save effort and time to change the status of wind turbines in the aggregated model. The proposed wind farm is composed of some smaller farms of permanent magnet synchronous generators (PMSGs) and others of squirrel cage induction generators (SCIGs).

Heat transfer characteristics and entropy generation for wing-shaped-tubes with longitudinal external fins in cross-flow

Sayed Ahmed E. Sayed Ahmed,Osama M. Mesalhy,Mohamed A. Abdelatief 대한기계학회 2016 JOURNAL OF MECHANICAL SCIENCE AND TECHNOLOGY Vol.30 No.6

A numerical study is conducted to clarify heat transfer characteristics, effectiveness and entropy generation for a bundle of wingshaped-tubes attached to Longitudinal fins (LF) at downstream side. The air-side Re a ranged from 1.8 x 10 3 to 9.7 x 10 3 . The fin height (h f ) and fin thickness (δ) have been changed as: (2 mm ≤ h f ≤ 12 mm) and (1.5 mm ≤ δ ≤ 3.5 mm). The analysis of entropy generation is based on the principle of minimizing the rate of total entropy generation that includes the generation of entropy due to heat transfer and friction losses. The temperature field around the wing-shaped-tubes with (LF) is predicted using commercial CFD FLUENT 6.3.26 software package. Correlations of Nu a , St a , and Bejan number (Be), as well as the irreversibility distribution ratio (Φ) in terms of Re a and design parameters for the studied bundle are presented. Results indicated that, installing fins with heights from 2 to 12 mm results in an increase in Nu a from 11 to 36% comparing with that of wing-shaped tubes without fins (NOF). The highest and lowest values of effectiveness (ε) at every value of the considered Re a range are occurred at h f = 6 mm and (NOF), respectively. The wing-shaped-tubes heat exchanger with h f = 6 mm has the highest values of (ε), efficiency index (η) and area goodness factor (G a ) and also the lowest values of Φ and hence the best performance comparing with other arrangements. The minimum values of Φ are occurred at h f = 6 mm. (Be) decreases with increasing Re a for all studied h f . The heat transfer irreversibility predominates for (1800 ≤ Re a ≤ 4200) while the opposite is true for (6950 < Re a ≤ 9700). δ has negligible effect on Nu a and heat transfer irreversibility. Comparisons between the experimental and numerical results of the present study and those, previously, obtained for similar available studies showed good agreements.

A New Soft-Fusion Approach for Multiple-Receiver Wireless Communication Systems

Ashraf M. Aziz,Ahmed M. ElBakly,Mohamed H.A. Azeem,Gamal A. Hamid 한국전자통신연구원 2011 ETRI Journal Vol.33 No.3

In this paper, a new soft-fusion approach for multiple-receiver wireless communication systems is proposed. In the proposed approach, each individual receiver provides the central receiver with a confidence level rather than a binary decision. The confidence levels associated with the local receiver are modeled by means of soft-membership functions. The proposed approach can be applied to wireless digital communication systems, such as amplitude shift keying, frequency shift keying, phase shift keying, multi-carrier code division multiple access, and multiple inputs multiple outputs sensor networks. The performance of the proposed approach is evaluated and compared to the performance of the optimal diversity, majority voting, optimal partial decision, and selection diversity in case of binary noncoherent frequency shift keying on a Rayleigh faded additive white Gaussian noise channel. It is shown that the proposed approach achieves considerable performance improvement over optimal partial decision, majority voting, and selection diversity. It is also shown that the proposed approach achieves a performance comparable to the optimal diversity scheme.