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RISS 인기검색어
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- 저자 : 金禮和(Rea Hwa Kim) (검색결과 2 건)
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國內 野生茶의 잎과 줄기 절편 培養에 따른 Callus 誘導
李善河(Seon Ha Lee),金禮和(Rea Hwa Kim),盧一燮(Ill Sup Nou) 한국차학회 1996 한국차학회지 Vol.2 No.1
For callus induction of Korean wild tea plants, leaf and stem segments were cultured on MS medium supplemented with different phytohormones. 1) The stem segments were heavily contaminated than the leaf segments, however, survival rates of the callus induced from stem segments were higher than those from leaf segments. 2) The callus induction rates from leaf segment were 42.2%, 35.2%, and 30.0% for 1st, 2nd, and 3rd leaf, respectively. 3) The callus induction rates from stem segment were higher in younger stems than in older ones. The induction rates were 58.4%, 56.0%, 50.0%, 45.2%, and 30.0% for 1st, 2nd, 3rd, 4th, stem and epidermal layer, respectively. 4) The callus induction rates from the epidermal layer were less than 60% of those from the intact stems. 5) The callus was more effectively induced when MS medium was supplemented with a combination of IBA and BA than with 2, 4-D and NAA.
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RAPD marker를 利用한 韓國 野生 茶나무 및 日本 茶品種의 同定
李善河(Seon Ha Lee),崔鶴淳(Hak Soon Choi),金禮和(Rea Hwa Kim),李孝淵(HyoYeon Lee),盧一燮(Ill Sup Nou) 한국차학회 1995 한국차학회지 Vol.1 No.1
The genetic similarity was evaluated by RAPD analysis between Kore-an wild tea plants and Japanese green tea cultivars. The optimal ampli-fication conditions were 50ng of template DNA, 0.5U of Taq DNA poly-merase, 2mM of Mg²+ in 25ul reaction buffer and the annealing tempera-ture of 45˚C. A total of 212 bands were generated by 20 primers of ar-bitrary sequence. The size of the amplified fragments ranged from about 250bp to 3000bp. Korean wild plant- and Japanese cultivar-specific RAPD markers comprised 18.9%(40 fragments) of the 212 fragments. The genetic distance value was measured as Euclidian value for pairwise comparisions among 23 Korean wild tea plants, or among all of the 48 samples. Among of the Korean wild plants, the minimum Euclidian dis-tance value(14.0) was obtained between sample no. 4(Songgwangsa) and no. 9(Daeheungsa), and the largest value(46.5) between sample no. 1(Baegyangsa) and no. 2(Cheoneunsa). And in case of all among the total of 48 samples, the minimum value(13.0) was obtained between sam-ple no. 24(Yabukita) and no. 48(Yaeho) of Japanese cultivars, the larg-est value(78.8) between sample no. 1(Baegyangsa) and no. 8(Geumsansa) of Korean plants. By Ward method, when the Euclidian distance value of 45 or less was used as the criterion, all of the 48 sam-ples can be grouped into 5. The dendrograms generated by Ward meth-od of cluster analysis, suggested that the Korean wild plants and Japa-nese cultivars have a close genetic relationship, and suggested that the genetic base of Japanese cultivars was narrower than that of Korean wild plants. Among the 44 RAPD products detected in this genetic study, 34 markers(77.3%) were found to be inherited to F₁progeny.
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