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C57BL6 마우스에서 복합생약제인 황금궁의 육모 효과
허진주(Jin Joo Hue),리란(Lan Li),유설혜(Sul Hye Lyu),백인정(In Jeoung Baek),연정민(Jung Min Yon),남상윤(Sang Yoon Nam),윤영원(Young Won Yun),황석연(Seock Yeon Hwang),홍진태(Jin Tae Hong),이범준(Beom Jun Lee) 대한약학회 2005 약학회지 Vol.49 No.6
Hwanggumgung (HGG) is a hair-care product which is composed of several plant extracts used in oriental medicine. This study was carried out to investigate effect of HGG on hair regrowth in a shaving model of C57BL6 mice. Five-week-old mice were acclimated for 1 week under 23±3℃, 50±10% relative humidity, and 12h of a light/dark cycle before beginning experiment. There were four experimental groups including distilled water (D.W., control), 10% ethanol (EtOH, vehicle control), a positive control of 3% minoxidil (MXD), and HGG for female and male mice, respectively. Six-weeks old mice were trimmed by electric clippers so as not to damage the skin. The next day, mice without visible scraches were selected, randomized and separated in groups of 11 mice. The test compounds were topically treated with 0.15㎖ per mouse per day for 21 days. The hair regrowth was photographically and histologically determined during the experimental period of 21 days. Enzyme activities of γ-glutamyl transpeptidase and alkaline phosphatase were also determined using a rate assay method. There were no clinical signs in all experimental groups. The topical application of 3% MXD and HGG in female mice promoted hair regrowth earlier and faster than the control groups. In male mice, the topical application of 3% MXD and HGG also accelerated hair growth compared with the controls. Ten percent ethanol also promoted hair growth faster than D.W group. The histology of hair growth in experimental groups was strongly associated with the hair regrowth. 3% MXD and HGG promoted elongation of hair follicles compared with the controls in both female and male mice. Activities of alkaline phosphatase and γ-glutamyl transpeptidase, enzymes related to hair growth, significantly increased after treatments of 3% MXD and HGG for 2 weeks in both female and male mice (p<0.05). These results suggest that HGG has hair growth promoting activities and it can be for treatment for alopecia.
Streptozotocin으로 유도된 당뇨 마우스에서 L-carnosine의 혈당강하 효과
허진주,김종수,김준형,남상윤,윤영원,정재황,이범준,Hue, Jin-Joo,Kim, Jong-Soo,Kim, Jun-hyeong,Nam, Sang Yoon,Yun, Young Won,Jeong, Jae-Hwang,Lee, Beom Jun 대한수의학회 2010 大韓獸醫學會誌 Vol.50 No.2
Carnosine is a dipeptide $(\beta-alanyl-L-histidine)$ found in mammalian brain, eye, olfactory bulb and skeletal muscle at high concentrations. Its biological functions include antioxidant and anti-glycation activities. The objectives of this study were to investigate anti-diabetic effects of carnosine as determined by blood glucose levels, glucose tolerance test (GTT), glycosylated hemoglobin, and serum biochemical and lipid levels in streptozotocin-induced diabetic mice. There were five experimental groups including normal (ICR mice), control (saline), and three groups of carnosine at doses of 6, 30, and 150 mg/kg b.w.. Carnosine was orally administered to the diabetic mice everyday for 12 weeks. There was no significant difference in body weight changes in carnosine-treated groups compared to the control. The treatments of carnosine at the dose of 6 mg/kg significantly decreased the blood glucose level compared with the control at 2 and 4 weeks. The treatments of carnosine at the doses of 6 and 30 mg/kg significantly decreased the blood glucose levels in GTT and glycosylated hemoglobin compared with the control. Carnosine significantly increased total proteins compared with the control. Carnosine at the dose of 6 mg/kg significantly decreased total cholesterol and triglyceride in the serum compared to the control. These results suggest that carnosine at a low level has a hypoglycermic effect resulting from reduction of blood glucose and that a carnosine-containing diet or drug may give a benefit for controlling diabetes mellitus in humans.
C57BL/6J db/db생쥐에서 여주 (Momordica Charantia)의 항당뇨 효과
정재황,이상화,허진주,이기남,남상윤,윤영원,정성훈,이영호,이범준,Jeong, Jae-Hwang,Lee, Sang-Hwa,Hue, Jin-Joo,Lee, Ki-Nam,Nam, Sang Yoon,Yun, Young Won,Jeong, Seong-woon,Lee, Young Ho,Lee, Beom Jun 대한수의학회 2008 大韓獸醫學會誌 Vol.48 No.3
Many herbal extracts have been reported to have a preventive or therapeutic effect of on diabetes mellitus. Momordica Charantia commonly known as bitter melon or karela has been reported to be a medicinal plant for treating various diseases including cancers and diabetes. The objectives of this study were to investigate anti-diabetic effects of bitter melon (BM) as determined by blood glucose levels, glucose tolerance test (GTT), insulin tolerance test (ITT), insulin and HbA1C activities in serum, serum biochemical and lipid levels, histopathology, immunohistochemistry and AMPK-${\alpha}2$ expression of skeletal muscle in male C57BL/6J db/db mice. There were four experimental groups including vehicle control, BM 10 mg/kg, BM 50 mg/kg, and BM 250 mg/kg. BM at doses of 10, 50, and 250 mg/kg was orally administered to the diabetic mice everyday for 8 weeks. The treatments of BM 10, 50, and 250 mg/kg significantly decreased the blood glucose level in the diabetic mice compared with vehicle control (p < 0.05). The treatments of BM 10 and 50 mg/kg significantly decreased the GTT, ITT and HbA1c levels in the diabetic mice compared with vehicle control (p < 0.05). All BM groups significantly decreased GOT, GPT, BUN, LDL and glucose levels in the diabetic mice compared with the vehicle control mice (p < 0.05). The livers of mice treated with the BM 10, 50, and 250 mg/kg showed a remarkable decrease in the number of lipid droplets compared with the vehicle control. The pancreas of mice treated with the BM 10, 50, and 250 mg/kg showed a remarkable increase in insulin concentration of ${\beta}$-cells compared with the vehicle control. In addition, the treatments of BM 10, 50, and 250 mg/kg actually increased the expression of AMPK-${\alpha}2$ compared with vehicle control. These results suggest that BM has a respectable anti-diabetic effect resulting from inhibition of blood glucose level and lipid level in serum and that consumption of BM may give a benefit for controlling diabetes mellitus in humans.
랫드의 간에서 다양한 농도의 아플라톡신 투여에 의한 DNA Adduct의 형성과 Ras의 발현양상
김태명(Tae Myoung Kim),허진주(Jin Joo Hue),리란(Lan Li),김대중(Dae Joong Kim),남상윤(Sang Yoon Nam),윤영원(Young Won Yun),이범준(Beom Jun Lee) 한국독성학회 2005 Toxicological Research Vol.21 No.4
Aflatoxins are produced by Aspergillus flavus, parasiticus that grows in improperly stored cereals. Aflatoxin B₁ (AFB₁) is a potent hepatocarcinogen in a variety of experimental animals including human beings. In spite of a high attention to the hepatocarcinogenecity of aflatoxins, the relative toxicity of other types (AFB₂, AFG₁ and AFG₂) of the toxins is not fully clarified. Sprague-Dawley male rats were orally administered with AFB₁, AFB₂, AFG₁ and AFG₂ at the dose of 250, 1250, and 2500 μg/kg body weight. Animals were then killed at 12, 24 or 48 hrs following aflatoxin adminstration. Subsequently the relative weight of liver was measured and histopathological examination on the liver was performed. Level of 8-OxodG and expression of ras gene in the liver was determined. The relative liver weights at high doses of AFB₁ and AFG₁ was significantly low. The treatment of AFB₁ at the high dose of 2500 ㎍/㎏ showed vacuolar degeneration and centrilobular hepatic necrosis with inflammatory cells. The pathological changes by AFB₂, AFG₁ and AFG₂ were not clearly found. The formation of 8-OxodG by AFB₁ increased in a dose-dependent manner up to 24 hrs after a single treatment of AFB₁ thereafter decreased to the level of the control. The treatments of AFB₂, AFG₁ and AFG₂ showed an inconsistent pattern in the formation of 8-OxodG in the liver of rats with increasing time. The expression of ras oncogene in the liver by AFB₁ at the dose of 1250 ㎍/㎏ was increased twice compared to the control. The treatments of AFB₂, AFG₁ and AFG₂ at all doses decreased the expression of ras in the liver. These results in the present study indicate that AFB₁ among aflatoxins with low comparable levels is the most toxic as determined by early biomarkers such as 8-OxodG formation and ras expression. However, the levels of 8-OxodG and ras as biomarkers were not useful to predict the relative hepatocarcinogenicity of aflatoxins to AFB₁ in the present model. Further studies are required to look for other biomarkers to predict carcinogenic potency of aflatoxins.
원저 : 철분 결핍 마우스의 대장암 형성에 대한 셀레늄의 효과
김준형 ( Jun Hyeong Kim ),조민행 ( Min Haeng Cho ),허진주 ( Jin Joo Hue ),강봉수 ( Bong Su Kang ),박현지 ( Hyun Ji Park ),남상윤 ( Sang Yoon Nam ),윤영원 ( Young Won Yun ),김종수 ( Jong Soo Kim ),이범준 ( Beom Jun Lee ) 한국수의공중보건학회 2010 예방수의학회지 Vol.34 No.4
Selenium (Se) obtained from dietary sources is an essential micronutrient for normal body function and it functions as an essential constituent of selenoproteins. We investigated the influence of Se on the formation of colonic aberrant crpyt foci (ACF) and tumor formation induced by azoxymethane (AOM) and dextran sodium sulfate (DSS) in male ICR mice. Five-week old ICR mice were acclimated for one week and fed on the low iron diet (LFe, 4.5 ppm) and different Se diet [Lse (0.02 ppm), Normal Se (0.1 ppm), HSe (0.5 ppm)] for 12 weeks. Animals received intraperitoneal injections of AOM (10㎎/㎏B.W. in saline weekly for 3 weeks), followed by 2% DSS (molecular weight 36,000~50,000) in the drinking water for a week. There were five experimental groups, including a normal control group, AOM/DSS, LFe+AOM/DSS, LFe+AOM/DSS+LSe, LFe+AOM/DSS+HSe. After sacrifice of animals, the total numbers of AC and ACF were measured in the colonic mucosa. The number of mice bearing tumors was expressed as tumor incidence rate. The iron and selenium liver concentration was measured using ICP-AES. Glutathione peroxidase (GPx) activity was determined using a GPx assay kit in the liver and colon. TUNEL and proliferating cell nuclear antigen (PCNA) staining were performed to examine the cell apoptosis and cell proliferation. In addition, immunohistochemistry of β-catenin was also performed on the mucous membrane tissue of colon. In AOM/DSS-induced colon carcinogenesis animal model, LFe diet decreased the number of 2.95±2.5 ACF/cm2 to 1.85±1.1 ACF/cm2 but it increased the total number of 5.06±4.2 AC/cm2 to 6.19±4.8 AC/cm2 compared with normal iron diet. In the iron-deficient mice, selenium did not affect the either the number of ACF or AC. The tumor incidence rate was higher in LFe diet groups than in normal iron diet group and high selenium diet weakly reduced the tumor incidence. Low selenium diet decreased the activity of GPx in the liver and colon. Apoptotic positive cells were decreased in the low selenium diet group. In addition, on the β-catenin staining, positive cells were increased in the low selenium diet group while they were decreased in the high selenium diet group. These findings indicate that the dietary levels of selenium was not highly enough to exhibit a significant protection against colon carcinogenesis in the iron-deficient mice. However, our results also indicate that dietary selenium might exert a protecting effect against colon cancer by increasing GPx activity and apoptosis and by inhibiting cell proliferation and β-catenin over-expression.