재배국의 (裁培菊) 추정원종에 (推定原種) 관한 연구 (4)

한창열(Chang Yawl Harn),이만상(Man Sang Lee) 한국식물학회 1968 Journal of Plant Biology Vol.11 No.1

Present investigation was carried out in order to make clear the fertility, morphological characters, and chromosome numbers of interspecific hybrids of Chrysanthemum Zavadskii, C. indicum, and C. lacandulaefolium. 1. Hybrids were not self-pollinated. When sib-crossed the fertility was 1.3∼19.3%. 2. F_2 individuals were variable in their morphological Characters. 3. Chromosome numbers of three putative parents were different from those reported previously: C. zawadskii 2n=36, C. indicum 2n=20, C. lavandulaefolium 2n=16, C. indicum × C. zawadskii 2n=28, C. zawadskii × C. indicum-1 2n=28, C. zawadskii × C. indicum-2 2n=28, C. zawadskii × C. indicum-3 2n=28, C. zawadskii × C. indicum-4 2n=28, C. zawadskii × C. indicum-5 2n=28, C. zawadskii × C. lavandulaefolium-1 2n=26, C. zawadskii × C. lavandulaefolium-2 2n=26.

고려인삼의 자성배우체형성에 (雌性配偶體形成) 관한 연구

한창열(Chang Yawl Harn),황종규(Jong Kyu Whang) 한국식물학회 1963 Journal of Plant Biology Vol.6 No.2

HARN, Changyawl and WHANG, Jongkyu(Coll, of Agr., Chonpuk National University) Development of female gametophyte of Panax ginseng. Kor. Jour. Bot. VI(2): 3-6, 1963. As a part of embryological studies of Panax ginseng, megasporangium and megagametophyte formations were investigated. Ovule is found to be anatropous. Small-sized nucellus is surrounded by thick layered single integument. As the embryo sac develops, the nucellus along with some parts of the inner epidermis of integument disintegrates and completely disappers at flowering stage. Embryo sac takes the type of typical Polygonum although antipodal cells disappear and polar nuclei fuse to form secondary nucleus before fertilization, Mature embryo sac consists of egg apparatus and large secondary nucleus lying adjacent to the egg. Besides the normal ovule, tiny incomplete ovule develops near the base of style. Frequently two normal ovules are formed in a single locule. Chromosome number counted is PMC is n=24.

왕벚의 기원에 관한 연구 1. 한라산자생올벚 , 재배왕벚 및 산벚의 전기영동형 (電氣泳動型) 비교

한창열(Chang Yawl Harn),김영진(Yung J . Kim),양서영(Shu Y . Yang),정희준(Hee J . Chung) 한국식물학회 1977 Journal of Plant Biology Vol.20 No.1

Horizontal starch gel electrophoresis in three buffers was used to compare the electrophoretic patterns in three cherry species, wild Prunus subhirtella, cultivated P. yedoensis and P. donarium. Electrophoretic patterns of glutamate oxaloacetate, transaminase-2(GOT-2), malate dehydrogenase-2(MDH-2), and phosphoglucose isomerase(PGI) in three species showed strong evidence that P. yedoensis might be originated by hybridization between P. subhirtella and P. donarium.

양란의 (洋蘭) 생장점배양에 관한 연구 (2) - 배양생장점에서 원괴체의 (原塊體) 발생과정

한창열 (Chang Yawl Harn) 한국식물학회 1968 Journal of Plant Biology Vol.11 No.3

Uging several varieties of Cymbidium, investigations were carried out to make clear how the protocormic tissue develops from the cultured explant. Enplant to be cultured were prepared in several ways: exclusively apical meristem, apical meristem dissected out with the basal part attached, axillary bud primordia in their initial stage of development, or apical or axillary bud dissected out as a whole etc. It was observed that protocorms or protocormic tissues were developed from the explant`s meristematic tissues regardless of where these tissues were located. Apical meristem, leaf primordia, leaf axil, or internodal part of young bud turned easily protocormic, while the scaly leaves of axillary bud or stem tissue of mother shoot turned quickly brwonish and died away. Both in axillary and apical bud explant alike, whether they were cultured whole or divided, some took quickly green color while others were slower, and some developed protocorms easily while others remained unchanged for months. Varietal difference as well as environmental factors seemed to be responsible for it. Further details should be clarified by histogenetical investigations.

양란의 (洋蘭) 생장점배양에 관한 연구

한창열 (Chang Yawl Harn) 한국식물학회 1970 Journal of Plant Biology Vol.13 No.1

Several days after culture, the parts around suspensor turned brown. In about 10 days the embryo started to form protocorm sending out hairs through seed coat. Around 20 days after culture, most of the protocorms emerged out of seed coat and some of them began to tate green color. When observed two months after culture, the protocorm took the characteristic top-shape feature.

Nicotiana tabacum 의 약배양에 (葯培養) 관한 연구 2

한창열(Chang Yawl Harn),김문자(Moon Ja Kim) 한국식물학회 1971 Journal of Plant Biology Vol.14 No.1

After topping, axillary buds of haploid plants derived from cultured anthers were treated with 0.4% aqueous solution of colchicine. Due to the high temperature and dry air at the time of treatment, most of the buds perished. A few months after the colchicine application, however, several shoots arose from the places where the dead buds were originally located. These shoots were mostly diploid. Induction of adventive shoots from the colchicine-treated-axils was supposed to be rather effective method of obtaining diploid shoots from haploid plants. The diploid plants had larger floral organs than the haploid plants, and had good pollen fertility and seed setting. 24 bivalent chromosomes were observed at MI of the PMC`s.

Solanum nigrum L. 의 약배양에 관한 연구 2

한창열(Chang Yawl Harn),김문자(Moon Ja Kim),이병기(Byung Ki Lee) 한국식물학회 1971 Journal of Plant Biology Vol.14 No.2

Haploid calli obta-ined from microspores of Solanum nigrum were cultured on two kinds of medium, Callus-inducing medium and Differentiation medium, in order to conduct histological studies of callus and examine differentiation of plantlets. On the callus-inducing medium the calli grew rapidly. The bulk of callus mass was light brown colored Wet callus covered on the surface with thin layers of rough and gleaming White callus. The wet callus was consisted of parenchyma and meristematic tissues, while the white callus had no meristematic tissues. Large parenchyma cells, by successive divisions, became multicellular or poly nucleate cells which developed later to be meristematic tissues. The calli embedded on the differentiation medium quickly turned to dark brown color. Plantlets, however, came out later from these blackened callus mass. In the callus sectioned about ten weeks after imbedding on the differentiation medium, radially elongated tissue, concentric tissue, epidermis, tracheid-like structure, and plant primordia were observed.

수도단간변이계통의 (水稻短稈變異系統) 형태 및 규산함량에 관한 연구

한창열(Chang Yawl Harn),원종락(Jong Lak Won),최광태(Kwang Tae Choi) 한국식물학회 1971 Journal of Plant Biology Vol.14 No.3

Various types of short culm rice mutants were obtained by means of gamma-ray irradiation. In the present paper morphological observation and analysis of silicate content of the mutants are reported. 1. Short culm type had more useful characters than bushy and dwarf types. 2. In short culm and bushy types the number of nodes both above and under ground was similar to the mother varieties, while in dwarf type it decreased. 3. In short culm types the variation of length of above-ground culm and internodes tended to vary relative to the mother varieties. 4. Positive correlation was found between culm length and the first and fourth internode length in short culm type. 5. Silicate content increased in short culm type - the increment being variety specific.

담배 반수성의 (半數性) 유엽배양에 (幼葉培養) 관한 연구

한창열 (Chang Yawl Harn) 한국식물학회 1972 Journal of Plant Biology Vol.15 No.1

Young haploid leaf derived from the anthers of tobacco plant was cultured and plantlets of various ploidies were obtained. When the leaf was put on the medium supplemented with kinetin as growth regulator, plantlets developed directly from the leaf, and the plants coming out in early stage of culture were all haploid. Plants developing in later stage were mostly haploids with some exception of diploid and aneuploid. Leaves were also cultured on the callus-inducing media supplemented with 2,4-D and kinetion, and the calluses were sub-cultured for six months. Plants developed from these calluses were mostly aneuploids of various chromosome numbers. In view of the fact that the plants directly developed from the leaf were all haploid, the tissue of the original leaf explant was assumed to be uniform as far as chromosome number was concerned. On the other hand, it seemed that the occurrence of various ploidies in the plants derived from the calluses of same origin was the result of the influence of in vitro culture. Apical meristem tissues and various multicellular bodies were formed in the epidermal and inner mesophyll tissues as well as in the sub-epidermal cells.

벼의 원형질체 융합에 관한 연구

한창열(Chang Yawl Harn),최장경(Chang Kyung Choi) 한국식물학회 1972 Journal of Plant Biology Vol.15 No.4

Rice callus was treated with 0.56M sucrose solution mixed with 5% pectinase and 10% cellulase, and the protoplasts isolated were transferred to 0.25M sodium nitrate to induce protoplasmic fusion. Callus tissues were macerated well and degradation of cell walls also proceeded satisfactorily. When the protoplasts were transferred to sodium nitrate solution, many giant roundish protoplasts and some multilobed complex protoplasmic bodies were observed. Most of the fusions took place immediately after the protoplasts were transferred to sodium nitrate. Some multilobed protoplasts which failed to fuse in the initial stage took longer time, about two hours, to get completely fused and rounded-off. Multilobed protoplasmic bodies were invariably multinucleate, while giant round protoplasts had either several nuclei or had one nucleus of large size. Nuclear fusion, also, seemed to occur immediately after the protoplasts were transferred to sodium nitrate.