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벼 microarray를 이용한 유전자발현 profiling 연구동향
윤웅한,김연기,김창국,한장호,이태호,김동헌,이강섭,박수철,남백희,Yoon, Ung-Han,Kim, Yeon-Ki,Kim, Chang-Kug,Hahn, Jang-Ho,Kim, Dong-Hern,Lee, Tae-Ho,Lee, Gang-Seob,Park, Soo-Chul,Nahm, Baek-Hie 한국식물생명공학회 2010 식물생명공학회지 Vol.37 No.2
As the International Rice Genome Sequencing Project (IRGSP) was completed in 2005 and opened to the public, many countries are making a lot of investments in researches on the utilization of sequence information along with system development. Also, the necessity of the functional genomics researches using microarray is increased currently to secure unique genes related with major agricultural traits and analyze metabolic pathways. Microrarray enables efficient analysis of large scale gene expression and related transcription regulation. This review aims to introduce available microarrays made based on rice genome information and current status of gene expression analysis using these microarrays integrated with the databases available to the public. Also, we introduce the researches on the large scale functional analysis of genes related with useful traits and genetic networks. Understanding of the mechanism related with mutual interaction between proteins with co-expression among rice genes can be utilized in the researches for improving major agricultural traits. The direct and indirect interactions of various genes would provide new functionality of rice. The recent results of the various expression profiling analysis in rice will promote functional genomic researches in plants including rice and provide the scientists involved in applications researches with wide variety of expression informations.
SNP 마커를 이용한 벼 흰잎마름병 저항성 선발 효율 증진
신운철,백소현,서춘순,강현중,김정곤,신문식,이강섭,한장호,김현순,Shin, Woon-Chul,Baek, So-Hyeon,Seo, Chun-Sun,Kang, Hyeon-Jung,Kim, Chung-Kon,Shin, Mun-Sik,Lee, Gang-Seob,Hahn, Jang-Ho,Kim, Hyun-Soon 한국식물생명공학회 2006 식물생명공학회지 Vol.33 No.4
본 연구는 흰잎마름병 K1 레이스에 감수성인 상주찰벼와 저항성인 HR13721-53-3-1-3-3-2-2를 인공교배하여 육성된 F2, F3를 재료로 하천 Kl 레이스에 대한 저항성 검정과 SNP 마커를 이용한 유전자형 분석 및 저항성과의 연관성을 분석하였다. Kl 레이스에 대한 저항성 검정 결과 $F_2,\;F_3$에서 각각 이론적 분리비인 3:1, 1:1의 분리비를 나타냈으며 SNP 마커를 이용한 유전자형 분석은 16PFXa1 primer를 이용하여 유전자를 증폭한 후 Eco RV 제한효소 처리하여 다형성을 분석하여 저항성 및 유전자형을 확인할 수 있었다. K1 레이스에 대한 저항성 검정과SNP마커를 이용한 유전자형의 연관분석 결과 저항성과 마커간에 연관성이 일치하였으며, 특히 SNP 마커를 이용한 유전자형 분석에서는 K1 레이스에 대한 저항성 검정에서 알 수 없었던 $F_2$ 개체가 동형접합체인지 이형접합체인지를 판별할 수 있어 저항성 품종 육종을 위한 선발 효율을 높일 수 있었다. Discovery of single nucleotide polymorphisms (SNPs), including small insertions and deletions, is one of the hot topics in genetic research. The most common type of sequence variant consists of single base differences or small insertions and deletions at specific nucleotide positions. Significance of SNPs in rice is increasing for genetic research, positional cloning and molecular breeding. $F_2$ 170 lines and $F_3$ 194 lines derived from Sangjuchalbyeo/HR13721-53-3-1-3-3-2-2 Were used for Searching SNP markers related to bacterial blight resistance. Sangjuchalbyeo is susceptible to bacterial blight, but HR13721-53-3-1-3-3-2-2 has Xa1 gene resistant to bacterial blight. Individual lines were inoculated with $K_1$ race of bacterial blight and resistant or susceptible was evaluated after 3 weeks from inoculation. The genotypes of population were analysed by PCR-RFLP for SNP marker developing. The segregation of $F_2\;and\;F_3$ population showed almost 3:1, 1:1 ratio, respectively. Analysis of genotype using SNP marker is capable of confirming resistance for $K_1$ race and genotype through amplifying the gene using 16PFXal primer and digested the PCR product with Eco RV. There were close relation between resistance test for $K_1$ race and SNP marker genotype. Especially, DNA analysis using SNP marker is capable of judging homozygote/heterozygote in $F_2$ population compared with resistant test for Kl race. So, it seems to improve the selection efficiency in disease resistant breeding.
연구보문 : 자연과학 ; 자포니카 벼의 종자 저장단백질 유전자 구조 및 발현분석 연구 현황
윤웅한 ( Ung Han Yoon ),이정화 ( Jeong Hwa Lee ),이강섭 ( Gang Seob Lee ),김영미 ( Young Mi Kim ),한장호 ( Jang Ho Hahn ),지현소 ( Hyeon So Ji ),윤성원 ( Sung Won Yoon ),이종렬 ( Jong Yeol Lee ),김태호 ( Tae Ho Kim ) 한국국제농업개발학회 2012 韓國國際農業開發學會誌 Vol.24 No.3
Based on the outcome of the rice genome sequencing, a lot of researches for identification of genes underlying useful traits and their utilization are being carried out recently. Especially, rice seed storage proteins are used as a main nutrition source for human while they are used as the nitrogen source for plant growth during seed germination. Rice seed storage proteins are divided by solubility in solvent into weak acid and alkali soluble glutelin, alcohol soluble prolamin, and salt soluble globulin. The rice genome contains 11 glutelin genes and 33 prolamin genes. In the case of prolamin genes, 15 genes are located in 15 Mb region on the chromosome 5. Clustering of seed storage protein genes on the same chromosomal region indicates that they are evolved from the same chromosome or they share similar gene expression regulatory region. Only single globulin gene existed on the chromosome 5. As a result of tissue- specific expression pattern analysis of seed storage protein genes using microarray, glutelin and most of prolamin genes were expressed only in seed endosperm, while a few prolamin and the globulin genes are expressed in seed endosperm and germinating seed. As a result of isolating promoter regions of the seed storage protein genes and analyzing their expression pattern in seed, glutelin type C1 promoter showed expression in whole seed, and glutelin type B5 and α-globulin promoter showed strong expression. This paper reviewed on the structure and expression profile of rice seed storage protein genes, and will provide information on the recent research status to the researchers who are studying on the improvement of seed traits and production of substances.