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하승모(Seung-mo Ha),한병욱(Byoung wook Han),김희봉(Hee bong Kim),안유민(Yoomin Ahn) 대한기계학회 2010 대한기계학회 춘추학술대회 Vol.2010 No.11
Microfluidic fuel cells are defined a group of fuel cells capable of operation within the framework of a microfluidic chip. This type of fuel cell operates without a physical barrier, such as a membrane, to separate the anode and the cathode, and can use both metallic and biological catalysts. In this study, we propose the microfluidic fuel cell integrated groove shape electrodes for improvement of the power density. The microfluidic fuel cell, composed of polydimethylsiloxane (PDMS) layer over a Pyrex glass substrate that contains a platinum groove shape electrodes, was designed and fabricated. Hydrogen peroxide is employed both as fuel and oxidant and dissolved in dilute sodium hydroxide and sulfuric acid solutions, respectively. Experiments were performed on the microfluidic fuel cells integrated various length electrodes. The maximum power density of groove shape electrode fuel cell higher than non-groove shape electrode fuel cell.
하승모(Seung-Mo Ha),조웅(Woong Cho),안유민(Yoomin Ahn),황승용(Seung Yong Hwang) 대한기계학회 2010 大韓機械學會論文集A Vol.34 No.12
중합효소 연쇄반응(PCR)을 수행하려면 세포 용해(cell lysis)와 DNA추출(DNA purification)과정이 포함된 시료 전처리 과정을 거쳐야 한다. 종래의 시료 전처리 과정은 계면활성제와 같은 세포용해 버퍼를 이용하거나 열 또는 전기적 방법으로 세포막 파열을 유도하여 세포벽을 깬 후에 잔여물 처리과정을 거쳐 DNA를 추출하게 된다. 본 연구에서는 마이크로 비드와 PDMS 기둥을 이용한 필터가 있는 시료 전 처리용 바이오칩을 설계 및 제작하였다. 또한 제작된 바이오칩을 사용하여 80℃ 에서 2분간 세포용해를 수행하고 DNA를 추출하였다. 칩에서 전처리과정을 거친 시료내의 DNA농도와 순도를 측정하고 DNA PCR과 겔 전기영동을 통해 시료 전처리용 바이오칩의 성능을 평가하였다. This paper describes a separable microfluidic device fabricated with PDMS (polydimethylsiloxane) and glass. The device is used for sample preparation involving cell lysis and the DNA purification process. The cell lysis was performed for 2 min at 80°C in a serpentine-type microreactor (20 μl) using a Au microheater that was integrated with a thermal microsensor on a glass substrate. The DNA that was mixed with other residual products during the cell lysis process was then filtered through a new filtration system composed of microbeads (diameter: 50 ㎛) and PDMS pillars. Since the entire process (sample loading, cell lysis reaction, DNA purification, and sample extraction) was performed within 5 min in a microchip, we could reduce the sample preparation time in comparison with that for the conventional methods used in biochemistry laboratories. Finally, we verified the performance of the sample preparation chip by conducting PCR (polymerase chain reaction) analysis of the chip product.
임현승(Hyeonseung Im),이수민(Sumin Lee),안유민(Yoomin Ahn),하승모(Seung-mo Ha),정성환(Sunghwan Jung),이성호(Sungho Lee) 대한기계학회 2010 대한기계학회 춘추학술대회 Vol.2010 No.11
Fluidic self-assembly is attractive solution to overcome packaging and integration challenges. This paper shows a new method of fluidic self-assembly with bubble of drag force. We fabricated the Si wafer with Cu micro pattern of 650㎛ x 650㎛ using photo lithography. Solder balls are attached on the Cu micro pattern. In this experiments, we used the solder ball size of 400㎛, 500㎛ and 600㎛ to measure the effects of self-alignment and capillary force. Solder ball was made by Sn96.5/Ag3/Cu0.5. Experiment was carried out in the liquid GALDEN D40 at 230℃which is higher solder ball melting point. Capillary forces from molten solder ball can be used to bond micro-scale block (950㎛ x 950㎛) in a self-assembly process. Bubble made by tube size is 1mm and velocity is 0.11㎧.