식품영양학 분야 : 가임기 여성의 분석과 산출에 따른 엽산섭취량 비교

진현옥,이정아,임현숙 한국가정과학회 1999 새 천년의 가정생활 문화 (1999년도 학술대회) Vol.- No.-

가임기 여성의 주요 엽산급원식품 및 동 식품의 엽산함량 분석

진현옥(Hyeon Ok Jin),임현숙(Hyeon-Sook Lim) 한국식품영양과학회 2001 한국식품영양과학회지 Vol.30 No.1

본 연구결과로부터 한국인 가임기 여성의 엽산 섭취에 주로 기여한 식품은 배추김치를 비롯한 콩나물, 열무김치, 상치, 깻잎, 무우 등 채소류군임을 알 수 있었다. 그리고 연령군별로 엽산 섭취에 기여한 식품이 약간 달랐다. 한편 100 g당 100 μg 이상의 엽산을 함유한 고엽산함유식품은 마른 김, 콘푸레이크, 시금치, 삶은 녹두, 볶은 참깨, 메추리알, 총각김치, 강남콩, 부추, 볶은 땅콩 등이었다. 엽산 기여도가 가장 높은 식품인 배추김치의 엽산 함량은 52.2 μg/100 g으로 중엽산함유식품이나 대부분의 연구대상자가 다량을 섭취해 엽산 섭취에 대한 기여도가 가장 높았다. 1회 섭취분량당 엽산함량이 가장 높은 식품은 시금치로 121.4 μg/70 g이었으며 역시 채소류의 엽산 함량이 대체로 많았다. 한국인 상용식품의 엽산영양가표를 완전히 구축하기 위해서는 엽산 함량이 알려지지 않은 식품에 대한 분석이 필요하겠고, 엽산 함량은 식품의 조리방법, 조리시간, 가공상태 및 저장상태에 따라 크게 달라지므로, 앞으로 이에 대한 분석도 이루어져야겠다고 생각된다. In this sudy, we determined major foods and food groups which contributed to the intake of folate in Korean child-bearing women. Folate content of the major foods was also analyzed. A total of 91 healthy women with childbearing potential participated. They were divided into one of three groups by age; A (15~24 yrs), B (25~34 yrs) and C (35~49 yrs). Food consumption data of the subjects were obtained by the 24-hr recall method. The rate of contribution of each food to the total intake of dietary folate was determined. Seventy major foods for folate were selected from the 178 foods they consumed. Two sets of the 70 foods were collected from different markets. After extracting folates from the foods by heating with a HEPES-CHES buffer, the samples were treated with α-amylase, protease and folate conjugase simultaneously. Folate content was determined by a microbiological method using Lactobacillus casei (ATCC 7469). The most contributable food to folate intakes was Korean cabbage kimchi followed by laver, soybean sprout, rice, yolmu kimchi, eggs, lettuce, perilla leaves, sea mustard, and radish root. Food items that contributed to folate intake were slightly different among the age groups. Although the folate content of Korean cabbage kimchi was 51.6 μg/100 g, but due to the high amount of intake, it was ranked the first major food providing folate. High folate foods containing over 100 μg/100 g were laver, corn flakes, spinach, mungbean, sesame, quail's eggs, small radish kimchi, kidney beans, leeks and peanuts. However, based on the serving size, spinach, corn flakes, leeks, sweet potato, pepper leaves, quail's eggs, crown daisy, small radish kimchi and perilla leaves contributed above 50 μg of folate per serving size.

Heme Oxygenase-1 Determines the Differential Response of Breast Cancer and Normal Cells to Piperlongumine

이하나,진현옥,김진희,박진아,김지영,김보라,김원기,홍성은,이윤한,장윤환,홍석일,홍영준,박인철,서영준,이진경 한국분자세포생물학회 2015 Molecules and cells Vol.38 No.4

Piperlongumine, a natural alkaloid isolated from the long pepper, selectively increases reactive oxygen species production and apoptotic cell death in cancer cells but not in normal cells. However, the molecular mechanism underlying piperlongumine-induced selective killing of cancer cells remains unclear. In the present study, we observed that human breast cancer MCF-7 cells are sensitive to piperlongumine-induced apoptosis relative to human MCF- 10A breast epithelial cells. Interestingly, this opposing effect of piperlongumine appears to be mediated by heme oxygenase-1 (HO-1). Piperlongumine upregulated HO-1 expression through the activation of nuclear factorerythroid- 2-related factor-2 (Nrf2) signaling in both MCF-7 and MCF-10A cells. However, knockdown of HO-1 expression and pharmacological inhibition of its activity abolished the ability of piperlongumine to induce apoptosis in MCF-7 cells, whereas those promoted apoptosis in MCF- 10A cells, indicating that HO-1 has anti-tumor functions in cancer cells but cytoprotective functions in normal cells. Moreover, it was found that piperlongumine-induced Nrf2 activation, HO-1 expression and cancer cell apoptosis are not dependent on the generation of reactive oxygen species. Instead, piperlongumine, which bears electrophilic ,-unsaturated carbonyl groups, appears to inactivate Kelch-like ECH-associated protein-1 (Keap1) through thiol modification, thereby activating the Nrf2/HO-1 pathway and subsequently upregulating HO-1 expression, whichaccounts for piperlongumine-induced apoptosis in cancer cells. Taken together, these findings suggest that direct interaction of piperlongumine with Keap1 leads to the upregulation of Nrf2-mediated HO-1 expression, and HO-1 determines the differential response of breast normal cells and cancer cells to piperlongumine.

한국인 가임기 여성의 영양소 섭취량 및 식사 다양성 평가

임현숙,이정아,진현옥 한국가정과학회 2000 한국가정과학회지 Vol.3 No.1

This study was performed to assess the extent of dietary diversity and nutrient intakes of Korean child-bearing women and to investigate the relationships between dietary diversity indices and nutrient intakes. A total of 91 women aged 18 to 48 years old participated. They were divided into four groups by age; 15 subjects in teens, 20 in twenties, 32 in thirties and 24 in forties. Mean energy intake was 1638±471㎉/day and it was low as 81.4% of the Korean RDA for energy. Mean intakes of folate, calcium and iron were also low as 81.7%, 72.6% and 57.2% of the RDAs. On the other hand, protein, vitamin A, thiamin, riboflavin and vitamin C were consumed close to or above the RDAs. It was obvious that the subjects in teens and twenties took less all of the nutrients as well as energy than those in thirties and forties. Mean nutrient adequacy ratio(MNAR) was 0.77 and showed a trend to raise with increasing age. Thus MNAR of the teens was the lowest and that of the forties was the highest among the age groups. The results in this study support the previous findings that nutrient intakes of women in teens and twenties were poorer compared to those in thirties and forties. Mean dietary diversity score(DDS), dietary variety score(DVS) and dietary frequency score(DFS) were 4.1. 11.6 and 14.7, respectively. DVS and DFS of the teens and twenties were lower than those in thirties and forties. DDS significantly correlated to the intakes of energy and calcium only, however. DVS and DFS had significant relationships to the intakes of all nutrients. The MNAR significantly related to DVS and DFS, but not DDS. In conclusion, the intakes of energy, folate, calcium and iron of child-bearing women were not sufficient and their nutrient adequacy highly correlated with dietary diversity. It indicates that to consume various foods helps to enhance the nutritional status.

가임기 여성의 엽산 섭취량 및 엽산영양상태

임현숙(Lim Hyeon-Sook),진현옥(Jin Hyeon-Ok),이정아(Lee Jeong-A) 韓國營養學會 2000 Journal of Nutrition and Health Vol.33 No.3

Increased Expression of FosB through Reactive Oxygen Species Accumulation Functions as Pro-Apoptotic Protein in Piperlongumine Treated MCF7 Breast Cancer Cells

Keun-Cheol Kim,Jin-Ah Park,Han-Heom Na,진현옥 한국분자세포생물학회 2019 Molecules and cells Vol.42 No.12

Piperlongumine (PL), a natural alkaloid compound isolated from long pepper (Piper longum), can selectively kill cancer cells, but not normal cells, by accumulation of reactive oxygen species (ROS). The objective of this study was to investigate functional roles of expression of SETDB1 and FosB during PL treatment in MCF7 breast cancer cells. PL downregulates SETDB1 expression, and decreased SETDB1 expression enhanced caspase 9 dependent-PARP cleavage during PL-induced cell death. PL treatment generated ROS. ROS inhibitor NAC (N-acetyl cysteine) recovered SETDB1 expression decreased by PL. Decreased SETDB1 expression induced transcriptional activity of FosB during PL treatment. PARP cleavage and positive annexin V level were increased during PL treatment with FosB overexpression whereas PARP cleavage and positive annexin V level were decreased during PL treatment with siFosB transfection, implying that FosB might be a pro-apoptotic protein for induction of cell death in PL-treated MCF7 breast cancer cells. PL induced cell death in A549 lung cancer cells, but molecular changes involved in the induction of these cell deaths might be different. These results suggest that SETDB1 mediated FosB expression may induce cell death in PL-treated MCF7 breast cancer cells.

Development Hybrid Scaffold Using Bladder Submucosa Matrix and Alginate

최진현,임현주,정호윤,전소영,권태균,임정옥 한국고분자학회 2011 한국고분자학회 학술대회 연구논문 초록집 Vol.2011 No.1

생체적합성 및 생체안정성이 향상된 이식용 합성 수화젤

최진현(Choe Jin Hyeon),박보형(Park Bo Hyeong),한영아(Han Yeong A),김수정(Kim Su Jeong),임정옥(Im Jeong Og) 한국고분자학회 2004 폴리머 Vol.28 No.1

Bladder Submucosa Matrix-Alginate 융복합 지지체

임현주,최정연,노혜정,정호윤,임정옥,전소영,권태균,최진현 한국조직공학과 재생의학회 2011 조직공학과 재생의학 Vol.8 No.1s

It is known that in bladder submucosa matrix (BSM), lots of bioactive substances which contribute to tissue regeneration such as growth factor, adhesion molecule, and modulators of coagulation are contained. In this study, we prepared BSM-alginate hybrid scaffolds, investigated basic properties such as morphology and cell viability,and evaluated their function with human skeletal muscle cells (hSkMCs) in vivo, considering their utility as scaffolds for muscle regeneration. The 135 proteins such as muscle-specific intermediate filament desmin, cardiac muscle α actin 1, cytoskeletal β‚ actin, keratin 1, decorin, and vimentin were identified in BSM. Porosity of the scaffold was reduced by incorporating BSM, suggesting the density of scaffold was increased. Excellent cell viability and cytocompatibility of alginate scaffold was maintained in the case of BSM-alginate scaffold. The immunofluorescence and immunochemical staining of the BSM-alginate scaffolds implanted with hSkMCs in subcutaneous spaces of balb/c-nude mice confirmed that the presence and the migration of cells and tissues, as well as the structural stability of scaffolds in vivo, were predominant as compared with the alginate scaffolds. Conclusively, BSMalginate scaffold is considered a biomaterial feasible for stem cell tissue engineering.