나노압전소자의 현황과 전망

정성윤,김선영,김연상,Jeong, Seong-Yun,Kim, Seon-Yeong,Kim, Yeon-Sang 한국전기전자재료학회 2012 전기전자재료 Vol.25 No.2

LED조명 기반 가시광통신 기술 동향

정성윤,김현철,Jeong, Seong-Yun,Kim, Hyeon-Cheol 한국광학기기산업협회 2014 光學世界 Vol.149 No.-

적조생물 살조세균 탐색 -IV. 살조세균 Micrococcus sp. LG-5가 생산하는 살조물질의 특성과 해양생물에 미치는 영향-

정성윤,박영태,김무찬,최석철,성희경,김재영,김태운,이원재,JEONG Seong-Youn,PARK Young-Tae,KIM Mu-Chan,CHOI Seok-Cheol,SEONG Hee-Kyung,KIM Jai-Young,KIM Tae-Un,LEE Won-Jae 한국수산과학회 2000 한국수산과학회지 Vol.33 No.4

적조생물을 살조시키는 해양세균 Microcorcus sp. LG-5가 생산하는 세포외 분비 살조물질의 최적 생성조건, 살조물질의 특성과 한외여과 분획별 살조활성 및 해양생물에 미치는 영향을 조사함으로서 자연생태 조화형, 환경 친화적 적조방제 기술개발의 기초 자료를 제공하고자 연구한 결과를 요약하면 다음과 같다. Micrococcus sp. LG-5의 살조물질 생성 최적온도는 $20{\~}30^{\circ}C$로 살조활성이 $100{\%}$였으며, 살조물질 생성 최적 pH는 7.0, 최저 염농도는 $3.0{\%}$였다. 또한 세포외 분비 살조물질의 $IC_(50)$ 값은 $0.482{\%}$였다. 세포외 분비 살조물질의 열 안정성을 실험한 결과, 열처리한 배양여과액 $1{\%}$ 첨가시 12시간 후에 $31.4{\%}$, 24시간 후 $79.7{\%}$, 36시간 후 $94.6{\%}$의 살조활성을 보였고 $5{\%}, 10{\%}$의 경우 각자 36시간, 24시간 후에 $100{\%}$ 살조되었으며, 열처리하지 않은 배양여과액을 넣었을 때와 비교해볼 때 살조력에 유의한 차이는 없었다. 이와 같이 Micrococcus sp. LG-5가 생산, 분비하는 살조물질은 열에 안정한 내열성 물질로 판단된다. 또한 대사산물의 pH 안정성은 $pH 6.0{\~}8.0$에서 거의 $100{\%}$의 살조활성을 보였으며 $pH 5.0{\~}10.0$에서 살조활성이 $85{\%}$ 이상으로 나타나 살조물질은 약산성과 알카리에서 살조활성이 안정하였다. 또한 한외여과의 결과, 분자량 $>10,000, 10,000{\~}1,000, <1,000$의 3개의 분획 모두 살조활성이 있었으며, 각 분획 모두 열에 대단히 안정하였다. 각 분획별 $IC_(50)$ 값은 분자량 1,000 이상에서 강한 살조활성이 나타났으며 분자량 1,000 이하에서는 상대적으로 낮은 살조활성을 보였다. Micrococcus sp. LG-5가 해양세균에 미치는 영향은 E. faecalis, E. coli, K. pneumoniae 및 V. alginolyticus에 대해서는 항균력을 보였으나, P. aeruginosa, P. fluorescens, S. typhi, S. aureus, V. cholerae 및 V parahaemolyticus에 대해서는 전혀 항균력을 보이지 않았다. 그리고 적조생물에 미치는 영향은 A. tamarense, E. gymnastica, G. catenatum, G. niikimotoi, G. sanguineum, G. impudicum, H. triquetra, H akashiwo, P. micans와 Pyramimonas sp.에 대해서는 살조활성을 보였으나 Chlamydomonas sp., C. closterium, P. mininum, P. triestimum, Pseudonitzschia sp.와 S. trochoidea에 대해서는 살조활성을 보이지 않았다. 즉, 적조생물에 따라 살조활성이 다양하게 나타나 종 특이성이 낮았다. 먹이생물에 미치는 영향은 1 galbana를 제외하고 실험한 모든 먹이생물에 별다른 영향이 없거나 오히려 성장을 촉진시켰고, 또한 살조세균 배양여과액을 넙치 사육수에 $10{\%}$까지 투여하였을 때 양식 넙치에 미치는 유해한 생리학적 영향은 없었다. 이상의 연구 결과 살조세균 Micrococcus sp. LG-5의 배양여과액은 조사된 적조생물을 제외한 해양세균, 치${\cdot}$자어의 먹이생물 및 양식 넙치에 유해한 영향은 미치지 않을 것으로 판단된다. An algicidal bacterium, Micrococcus sp. LG-5 against the harmful dinoflagellate, Cochlodinium polykrikoides was isolated. The optimal conditions for the highest algicidal activity of bacterial culture filtrate showed in the range of $20{\~}30^{\circ}C$, at pH 7.0 and $3.0{\%}$ of NaCl concentration. In addition, $IC_(50)(mean of 50{\%} inhibitory concentration)$ of the culture filtrate against C. polykrikoides after incubation of 5 days was $0.482{\%}$. To investigate heat and pH stability of the culture filtrate of Micrococcus sp. LG-5, the culture filtrate ($pore size, 0.1 {\mu}m$) was heated to $121^{\circ}C for 15 min$ and adjusted pH from 2.0 to 10.0. There were no significant changes in algicidal activity by heat treatment and the pH change between pH from 5.0 to 10.0. The algicidal substances produced from Micrococcus sp. LG-5 were mainly detected in the fraction of $10,000{\~}1,000$ MWCO (molecular weight cut-off). The culture filtrate of Micrococous sp. LG-5 showed antimicrobial activity against Enterococcus faecalis, Escheiichia coli, Uebsiella pneunioniae and Vibrio altinolyticus, but did not show against Pseudomonas aeminosa, P. Buorescens, Salmonella typhi, Staphylococcus aureus, V. cholerae and V parahaemolyicus. The culture filtrate of Micrococcus sp. LG-5 was examined against 16 phytoplankton species and showed the algicidal activity against Ajexandzium tuarense, Eutreptiella Drnnastin, Gymnodinium catenatum, G. mikimotoi, G. sanguineum, eyodinium impuaicum, Heterocapsa triquetra, Heterosipa akashiwo, Prorocentrum micans and Pyraminonas sp.. However no algicidal effects of Micrococcus sp. LG-5 were observed against Chlamydomonas sp., Cylindrotheoa closterium, P. mininum, P. triestimum, Pseudonieschia sp. and Sczipuiella trochoidea. On the other hand, algicidal activity on the tested marinelivefood was not detected except for Isochrysis galbana. In addition, physiological responses of cultured olive flounder (Paralichthys oliraceus) exposed to $1 and 10{\%}$ of the culture filtrate of Micrococcus sp. LG-5 were measured. There were no clear changes in AST, GGT, creatinine, urea, total cholesterol, total protein, albumine, $Mg^(+2), Ca^(+2), Na^+, K^+, and Cl^-$. These results indicate that olive flounders were not affected when they were exposed to the culture filtrate of Micrococcus sp. LG-5.

MISO 구조 회분식 공정에서의 중간 저장조 설계

정성윤,이의수 ( Seong Yoon Jeong,Euy Soo Lee ) 한국화학공학회 1996 화학공학의이론과응용 Vol.2 No.2

제품이상을 고려한 회분식 공정에서의 중간저장조 설계

정성윤,이경범,이의수 ( Seong Yoon Jeong,Gyeongbeom Yi,Euy Soo Lee ) 한국화학공학회 1998 Korean Chemical Engineering Research(HWAHAK KONGHA Vol.36 No.5

Batch process whih is operated in unsteady state mode is subject to various process imbalances. Consequently intermediate storage. vessel can take an important role for mitigating parameter variations, increasing plant availability and providing flexibility in sequencing and scheduling. A mathematical model of intermediate storage vessel sizing in parallel batch units subject to batch failure is derived. The case in which the inlet flow rate to the storage vessel is greater than or equal to the outlet flow rate from the storage vessel followed by the opposite case is considered. Two operation mode is considered, that is, in-phase operation and out of-phase operation. In phase operation refers to the delay times in parallel units while out of operation to distintly different delay times in the set of units. Analytical expressions for the limiting volume are obtained for several network configurations, i.e. multiple, input single output(MISO) system, multiple input multiple output(MIMO) system and serial system

적조생물 살조세균 탐색 -III. 유해성 적조생물 Cochlodinium polykrikoides에 대한 Micrococcus sp. LG-5의 살조 효과-

정성윤,박영태,이원재,JEONG Seong-Youn,PARK Young-Tae,LEE Won-Jae 한국수산과학회 2000 한국수산과학회지 Vol.33 No.4

최근 남해안 적조의 주요 원인생물인 C. polykrikoides를 살조 시키는 해양세균을 마산만의 적조발생 해역에서 분리, 동정하고 살조범위를 조사하였다. 분리 균주 중 살조활성이 우수한 Micrococcus sp. LG-5의 성장 특성과 살조효과에 대해 조사하여 해양 미생물을 이용한 자연생태 조화형, 환경 친화적 적조방제 기술 개발의 기초 자료를 제공하고자 연구한 결과를 요약하면 다음과 같다. 살조세균 Micrococcus sp. LG-5의 최적 성장조건은 $25^{\circ}C, NaCl 3.0{\%}, pH 7.0$이었으며, C. polykrikoides ($1.2{\times}10^4 cells/ml$)에 대한 Micrococcus sp. LG-5의 성장단계에 따른 배양여과액의 살조활성은 대수증식기 후기 및 정지기에 강하였다 대수증식기에는 C. polyklikoides를 $100{\%}$, 정지기에는 $82{\~}92{\%}$, 대수증식기 중기에는 $40{\~}80{\%}$, 유도기에는 $5{\%}$ 이하의 살조활성을 보여 Micrococcus sp. LG-5는 유도기를 거쳐 대수증식기에 살조물질을 활발히 생산하는 것으로 밝혀졌다. 또한 $0.4{\mu}m$의 cell culture insert를 삽입한 2조배양계를 이용하여 Micrococcus sp. LG-5의 살조유형을 조사한 결과, Micrococcus sp. LG-5는 $0.4{\mu}m filter$에 의해 C. polykirkoides와 격리된 상태에서도 C. polykirkoides를 살조시켜, 세포외로 물질을 분비하여 살조시키는 살조물질 분비형으로 밝혀졌다. 또한 Micrococcus sp. LG-5의 배양여과액의 최종농도가 $5{\%}, 10{\%}$가 되도록 첨가한 경우, C. polykrikoides의 개체수는 급격히 감소하여 3시간 경과 후에 centrol에 비해 각각 $30{\%}$와 $85{\%}$정도가 살조되었으며, $1{\%}$의 경우, 30시간 후에 $90{\%}$ 이상 살조되었다. 즉, Micrococcus sp. LG-5의 배양여과액의 양에 비례하여 살조효과가 증가하는 경향을 보였으며, 배양여과액 중에 C. polykrikoides를 사멸시키는 살조물질이 존재함을 알 수가 있었다. The algicidal effects of marine bacteria were investigated and a strain, which had the strongest algicidal activity against the harmful dinoflagellate, Cochiodinim polykrikoides was selected. The bacterium was isolated in seawater during the period of blooming of C. polykrikoides in Masan Bay. This algicidal bacterium was identified as Micrococcus sp. LG-5 by means of morphological and biochemical tests. The optimal culture conditions of Micrococcus sp, LG-5 were $25^{\circ}C,\;pH 7.0\;and\;3.0{\%}$ NaCl concentration. The algicidal activity of Micrococcus sp. LG-5 was significantly increased to maximum value in the late of logarithmic phase of cell cuture. In addition, the culture filtrate ($pore size,\;0.1{\mu}m$) of Microcoocus sp. LG-5 showed strong algicidal effects. The cell numbers of C. polykikoides were decreased from $1.2{\times}10^4 cells/ml\;to\;less\;than\;2{\times}10^3\;cells/ml$ within 3, 6, 30 hours at the concentrations of culture filtrate $10{\%},\;5{\%}\;and\;1{\%}$, respectively. These results indicated that the algicidal effect was mediated by certain substances released from Microooccus sp. LG-5.

알코올 의존 환자의 적혈구막 지질과산화 정도와 적혈구 평균 용적 및 간 효소 지표와의 관련성

정성윤,최인근,서국희,강희정,Jeong, Seong Yun,Choi, Ihn-Geun,Suh, Guk-Hee,Kang, Hee Jung 대한생물정신의학회 1998 생물정신의학 Vol.5 No.2

Objectives : Alcohol-induced oxidative stress has been known to injure various tissues or organs. This stress is related with free radicals which are produced as the result of long-term alcohol consumption. Malonyldialdehyde(MDA) is produced by the interaction of free radicals and cell membrane lipids, and indicates the degree of lipid peroxidation indirectly. The purpose of this study was to investigate the relationship between red blood cell(RBC) membrane lipid peroxidation by free radicals, and associated hepatic injuries and hematologic changes. Methods : Thirty-three subjects diagnosed as alcohol dependence according to DSM-IV diagnostic criteria were evaluated within 72 hours after discontinuing alcohol drinking. Clinical characteristics were evaluated by CAGE questionnaire and Korean Michigan Alcoholism Screening Test(MAST). RBC membrane MDA level was measured as the marker of RBC membrane lipid peroxidation. Aspartate aminotransferase(AST), alanine aminotransferase(ALT) and gamma-glutamyltransferase(GGT) were used as the biochemical markers of liver damage due to alcohol ingestion. The alcohol-induced hematologic change was assessed by mean corpuscular volume(MCV). Results : The results were as follows. Clinical characteristics were not different between two groups having normal and abnormal levels of AST, ALT, GGT or MCV. The levels of MDA were not correlated with the clinical characteristics and serum levels of AST, ALT and GGT. However, there was a significant correlation between the levels of MDA and the value of MCV(p=0.017). Conclusions : These findings suggest that oxidative stress in alcohol dependence may not be reflected in liver enzyme markers such as AST, ALT and GGT, but may be reflected in MCV.

BLE 비콘을 이용한 터널구간에서의 안전운행 정보시스템 개발에 관한 연구

정성윤(Seong-Yun Jeong) 한국산학기술학회 2015 한국산학기술학회 학술대회 Vol.2015 No.1

본 논문은 계속해서 증가하고 있는 도로터널에서의 교통사고를 예방하기 위해 터널구간을 통행하는 운전자에게 안전운행정보를 제공할 수 있도록 저전력 블루투스(Bluetooth Low Energy, BLE)방식의 비콘(beacon)을 이용한 안전운행정보시스템 개발을 구상하였다. BLE 비콘은 저전력, 초소형, 넓은 가용거리, No pairing, 1:N 서비스 등의 장점 때문에 차세대 근거리 데이터통신기술로 주목을 받고 있는 최신의 위치기반 서비스(LBS) 기술이다. 아울러 BLE 비콘을 이용한 안전운행정보시스템을 도입할 경우에 경제적 타당성을 분석하였다.

해양 방선균 유래 물질의 정제 및 항균 활성

정성윤(Seong-Yun Jeong) 한국환경과학회 2024 한국환경과학회지 Vol.33 No.3

형방지황탕이 사염화탄소 장기 투여로 유발된 백서 간경변의 예방에 미치는 영향

정성윤 ( Jeong Seong Yun ),김숭모 ( Kim Sung Mo ),임명현 ( Im Myeong Hyeon ),이준희 ( Lee Jun Hui ),박재현 ( Park Jae Hyeon ) 대한본초학회 2003 大韓本草學會誌 Vol.18 No.4

N/A Objective : In order to determine the prevention effects of oral administration with HyeungBungJiHumflangW3JHT) on the liver cirrhosis of rats induced by long time carbon tetrachloride injection, the changes of body weight and gains, absolute and relative liver weight, biochemistry of blood, histopathological changes, urine specific gravity and urine bilirubin levels were monitored after 10 weeks of concomitantly dosing of HBJHT extract(per os) and CCl₄(i.p). Method : The WBJHT extracts were daily dosed at 50(T1), l00(T2) and 200(T3)ms/kg for 10 weeks. Result : The body weight and gains were significantly and dose dependently increased in HBJHT treated group compared to that of CCl₄treated control group. The absolute and relative liver weight were significantly and dose dependently reduced in HBJHT treated group compared to that of CCl₄treated control group. Severe hepatocellular necrosis and ballooning, hyperplasia of connective tissue and subdue reduction of hepatic lobule, hyperplasia of bile duct in portal triad were dramatically decreased in HBJHT treated group compared to that of CCl₄treated control group in histopathological observation.The diameter of hepatic lobules was significantly enlarged, and in HBJHT treated group compared to that of cc14 treated control group, and the numbers of connective tissue, degenerative cell and bile duct were significantly and dose dependently decreased. The levels of serum AST, ALT, LDH, ALP, GGT and total bilirubin were SignificantJy and dose dependently reduced in HBJHT treated group compared to that of CCl₄treated control group. The urine specific gravities were significantly and dose dependently increased, and the urine bilirubin levels were decreased in HBJHT treated group compared to that of CCl₄treated control group. Conclustions : it is considered that HBJWT extracts have some favorable effect to prevent the induce of hepatic cirrhosis by CCl₄injection.