http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
油菜의 葯培養 硏究 Ⅰ. 基本培地 選定 및 葯의 適正 置床時期 究明
Jae Gyun Gwag(郭在均),Gun Sik Chung(鄭根植),Hyung Soo Suh(徐亨洙),Deog Sueng Lee(李德承),Bong Ho Lee(李奉鎬) 한국육종학회 1987 한국육종학회지 Vol.19 No.4
This study was conducted to confirm the effect of basal medium and to determine the adequate anther stage in anther culture of Brassica napus. Embryos were obtained only from Keller et al. medium and were detected in responding anthers after 13 days of culture and virtually completed by the day 38. The range of developmental stages varying from globular types to mature forms with developed cotyledons was observed. Microspore-derived haploids were characterized by slender flower bud and small flower size with poorly developed anther. Bud in which petal length varies from one-fourth to one-half of the length of the anther contained pollen was detected as the optimal culture stage.
양세준(Sae Jun Yang),이수관(Soo Kwan Lee),정근식(Gun Sik Chung) 한국육종학회 1992 한국육종학회지 Vol.24 No.3
Rice leaf discoloration in latest season planting under field condition proposed as a screening method for cold tolerance and its mode of inheritance detected here. The salient findings were summarized as follows; Rice leaf discoloration in latest season planting showed from the begining of cool temperature below margin to just before frost falls under field condition at adult stage. Components value, showing the dispersion of population, in rice leaf discoloration of 44 genotypes at weekly reading under two different condition indicated appropriate time to distinguish varietal difference. Highly significant correlation(r=0.7536) recognized between adult stage in latest season planting under field and seedling stage under cool water tank. Clear difference in rice leaf discoloration recognized among varietal groups. Indica having cold tolerance and Tongil type rice showed more greater degree and variance value in leaf discoloration than that of Japonica rice. Any significant varietal difference in leaf discoloration could not recognize in the Japonica rice. Based on the screening test, Milyang 83 as susceptible parent, and Iri 371, IR20654-RRR-15-1 as resistant parents were selected, then crossed reciprocally to detect inheritance mode of leaf discoloration. The mode of inheritance for rice leaf discoloration revealed that resistant characteristics was showed to be partial dominance, and the maternal effects could not be recognized.
벼 組織培養을 利用한 突然變異誘發 硏究 Ⅰ. 半數體에 대한 化學物質의 突然變異 誘起效果
Sae Jun Yang(楊世準),Byeong Geun Oh(吳秉根),Soo Kwan Lee(李壽寬),Gun Sik Chung(鄭根植) 한국육종학회 1988 한국육종학회지 Vol.20 No.3
This experiment was conducted to broaden the scope of mutation breeding and to introduce new source of variation by in vitro technology. Node tissue of haploid plants, derived from rice anther culture (CV. Dashukei 2) were cultured on the N6-Y1 basic medium supplemented with 10⁻⁵M NAA, 0.5 x 10⁻⁵M kinetin, and 5x10⁻⁵M ABA. Chemical mutagens, DES, EMS, Sodium Azide, and MNNG were applied in vitro system through this experiment. Diploidization of’haploid plants regenerated from node culture with mutagens were conducted. The progeny of regenerated diploid plants, M₂ generation, were evaluated in their major agronomic characters, and they were compared with A₂ generation derived from anther culture. Haploid plants directly regenerated from node tissue of haploid without callus formation. The ratio of plant regeneration was decreased when the concentration of chemical mutagens were increased. Autodiploidization ratio by tiller separation was 3.1% while that of 0.2% colchicine treatment for 12 hours was 23.0% under the field condition. Phenotypic patterns of 197 M₂ lines derived from node culture with mutagens showed that 80.2% M₂ lines were similar to their parental variety, but the others were different in some characters. For days to heading and culm length the means did not change but the range of variance was larger in M₂ lines than in parental variety. Distribution of days to heading in 146 M₂ lines derived from DES treatmat was similar to 137 A₂ lines derived from anter culture, but that of culm length between two populations showed a significant difference.
Sae Jun Yang(楊世準),Byeong Geun Oh(吳秉根),Gun Sik Chung(鄭根植),Jae Keun Sohn(孫再根) 한국육종학회 1988 한국육종학회지 Vol.20 No.1
The present study was focused on variability of qualitative characters in anther-dervied plants to obtain basic information for rice breeding through anther culture. Variability in the progeny of antherderived plants, A₂ lines, were compared with F₂ generation of the same cross combination. The frequency of tall in culm length was 79% in 594 F₂ plants, but 56% in 175 A₂ lines derived from Milyang 74/Dashukei 2 F₁ hybrids. The ratio of tall to short was 3:1 in F₂ population, and 1:1 in A₂ lines. Short culm of Milyang 74 was controlled by a recessive gene. There was no variants related to glaborous leaf blade of Dashukei 2 and resistant reaction to bacterial leaf blight, pathotype K₁, of Milyang 74 in A₂ lines. The glaborous leaf blade blade of Dashukei 2 controlled by a recessive gene and resistant reaction to bacterial leaf blight, pathotype K₁, of Milyang 74 controlled by a dominant gene were domonstrated in both F₂ population and A₂ lines derived from Milyang 74/Dashukei 2 F₁ hybrids.
十字花科植物의 原形質體培養과 融合에 關한 硏究 Ⅱ. 山東菜와 갓의 原形質體培養에 의한 植物體 再生
Byung Jun Park(朴炳俊),Hyung Soo Suh(徐亨洙),Gun Sik Chung(鄭根植),Jae Keun Sohn(孫再根) 한국육종학회 1987 한국육종학회지 Vol.19 No.3
The experiments were conducted to determine optimum concentration of various growth regulators in fresh and regeneration medium on which affect callus formation and plant regeneration from mesophyll protoplasts of Brassica campestris CV. “Koonweesandongchae” and Brassica juncea CV. “Kyungsangot”. The formation of microcallus from protoplast could be more successfully achieved when added weekly MS liquid medium containing 1.0mg/l NAA and 0.2mg/l BA in Brassica campestris and 0.2 mg/l 2.4-D, 1.0mg/l NAA and 0.2mg/l BA in Brassica juncea. Shoot differentiation from callus, 0.3cm in diameter was accomplished in MS medium containing 0.1mg/l IAA and 2.0mg/l Zeatin in Brassica campestris and 0.1mg/l IAA and 2.0mg/l Zeatin or 0.1mg/l NAA and 1.0mg/l BA in Brassica juncea.
Sae Jun Yang(陽世準),Byeong Geun Oh(吳秉根),Gun Sik Chung(鄭根植),Jae Keun Sohn(孫再根) 한국육종학회 1987 한국육종학회지 Vol.19 No.4
The present study was focused on variability of anther-derived plants in quantitative characters to obtain basic information for rice breeding through anther culture. Variability of progeny of anther-derived plants, A₂ Lines, were compared with F₂ generation of the same combination. The salient findings obtained are summarized as follows; The segregation and recombination of major characters such as days to heading, panicle length, and panicles per plant both 175 anther-derived A₂ lines and 594 F₂ plants derived from Milyang 74/Dashukei 2 F₁ hybrids presented normal distribution curves and refered to the inheritance of quantitative characters controlled by multiple genes. Expression of gene recombinants related to culm length of 48 anther-derived A₂ lines from singwangbyeo/Eunhatyeo F₁ hybrids showed similar to those of 406 F₂ plants, although average culm length of A₂ population was shorter than that of F₂ population. The segregation and recombination of fertility of 71 anther-derived A₂ lines from Milyang 62/Akibare F₁ hybids as remote cross showed clearly different from those of 578 F₂ plants. The curve of A₂ population appeared more centered, and tilted to higher fertility. This tendency was also appeared both anther-derived A₂ lines and F₂ population derived from Singwangbyeo/Eunhabyeo F₁ hybrids.
벼 葯培養 分化植物體의 變異性 Ⅳ. 통일형 / 자포니카형 F₁ 葯培養系統의 分類
Sae Jun Yang(楊世準),Byeong Geun Oh(吳秉根),Soo Kwan Lee(李壽寬),Gun Sik Chung(鄭根植) 한국육종학회 1989 한국육종학회지 Vol.21 No.1
Anthers of a cross between Tongil rice CV., Milyang 23 and Japonica rice CV., Nagdongbyeo were cultured on N6-Y1 medium, which Japonica rice was better adapted than Indica, to confirm a certain degree of screening effect of culture medium on the genetic expression of pollen plants. The salient findings obtained are summarized as follows; The majority of progeny of pollen plants, A2 lines, had horizontal leaf angle as intermediated type between parental varieties. Most of A2 lines also had the same leaf color of their parental varieties, but transgressive variation was expressed. Although most of A2 lines in grain shape distributed within parental range, transgressive variation in grain shape was also observed. The phenol color reaction of brown rice was coincide with seed integuments. The ratio of postive(+) phenol reaction to negative(-) was 1:1 in A2 population. It was considered that inheritance of the phenol color reaction was controlled by single dominant gene. The coloration of phenol solution after soaking seed integuments in 2% phenol for 5 days at 30℃ was more clearly than in brown rice. Transgressvie variation to milyang 23 observed both brown rice and seed integuments. Based on the results described above, the pollen grain of different genotype probably have the same totipotency into intact pollen plants. No significant selection effects was found during the process of anther culture. This results also provide useful information of rice breeding for Indica/Japonica hybrids through anther culture.
Sae Jun Yang(楊世準),Jae Keun Sohn(孫再根),Gun Sik Chung(鄭根植) 한국육종학회 1985 한국육종학회지 Vol.17 No.4
This experiment was studied to determine the effects of low temperature treatment on culturability and ploidy level of anther-derived plants in rice anther culture. N₆ basic medium supplemented with 2mg/ℓ of NAA and 1mg/ℓ of Kinetin used for callus induction, 0.2mg/ℓ of IAA and 1mg/ℓ of Kinetin for plant differentiation. Rice panicle with flag leaf sheath was pretreated by low temperature at 10℃ for 15 days. Cold-shocked anthers produced more callus induction and plant differentiation about 3 fold than those without pretreatment. Callus induced from cold-shocked anthers produced more haploid plants and less diploid plants than the callus induced from anthers without pretreatment. This tendency was recognized in a variety as well as some F₁ crosses.
十字花科 植物의 原形質體 培養과 融合에 관한 硏究 Ⅰ. 油菜의 原形質體 培養과 植物體의 再生
Byung Jun Park(朴炳俊),Bong Ho Lee(李奉鎬),Jae Kyun Shon(孫再根),Hyung Soo Suh(徐亨洙),Gun Sik Chung(鄭根植) 한국육종학회 1986 한국육종학회지 Vol.18 No.3
The experiments were conducted to identify several factors influencing mesophyll protoplast isolation, culture and plant regeneration of rape(Brassica napus cv. ‘Hanrayuchae’) The results obtained were summarized as follows ; It was required to preplasmolyse the mesophyll tissue before enzyme treatment to increase yield and stability of protoplasts. The most healthy protoplasts could be obtained when the tissue was treated with enzymes containing macerozyme 1.0% and cellulase 2.0% for 4 hours. Sustained cell division was obtained after culturing the protoplasts in liquid 8P-KM medium supplemented with 2.4-D 0.2mg/ℓ, NAA 1.0mg/ℓ, BAP 0.5mg/ℓ, glucose 0.4M, and mannitol 0.1M. First cell division was observed 5 days after plating and was sustained to eventually produce cell colonies after another 16 days. When micro-calli 4-5 weeks after plating were transferred to B₅ basal medium with NAA 1.0mg/ℓ, BAP 0.5mg/ℓ, sucrose 30g/ℓ and agarose 6g/ℓ, green calli were proliferated to be at least 0.5-1.0cm in diameter 8 weeks after initial plating. Multiple shoots were induced on MS medium supplemented with BAP 1.0mg/ℓ and NAA 0.01mg/ℓ. After transferring differentiated shoots onto MS medium without hormones, intact plants were eventually produced.