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소절편 조직의 신속보고 체계에 대한 연구 -새로운 조직 고정액과 표본제작-
장영진 ( Young Chin Chang ),김덕래 ( Duck Lae Kim ),이기헌 ( Ki Hyun Lee ),서동환 ( Dong Hwan Suh ),유건부 ( Keon Boo You ) 대한임상검사과학회 1999 대한임상검사과학회지(KJCLS) Vol.31 No.2
Background : Generally and traditionally surgical specimens are fixed in 10% buffered formalin at room temperature. But when we used 10% buffered formalin for tissue fixatives, Toxic gas and vapors are take place in laboratory. by this toxic gas and vapors we can``t breathing and working for long time, and more, tissue fixation procedures require various amount of reagents and time depending on specimen size and type. 80, we introduced new equipment and fixatives such as Microwave and Histochoice-formalin. Microwave technology can be used to enhance specimen fixation by promoting chemical bonding and penetration of reagents into tissues. As such microwaves have been used to decrease the time of tissue fixation. This study was undertaken to develop a rapid universal fixation procedure using a low concentration of formalin and microwave technology. Design : Microwave oven(Pe1co, 3440, 700 W) was used for ftxation, and this made monitoring and controlling of time and feasible temperature. A various tissue specimens such as brain, colon, lung, spleen, stomach and uterus were fIxed in 3% formalin in Histochoice (Amresco, 8010n OH). The specimens were processed at a various condition of powers, times and temperature in the specimens tissue processor that have vacuum function (Pathentreⓡ, 8handon, U8A). A comparative study for 10% neutral buffered formalin with or without microwave irradiation was done. We performed H&E stain, histochemistry, immunohistochemistry, and insitu hybridization using the rapidly fixed tissue sections and compared with routinely processed tissue sections. Results : The optimal temperature and irradiation time for suitable ftxation was determined to be 60 =1=30C at 300 =1=30 sec at 700 W. The optimal concentration for tissue ftxatives is 3% Histochoice-formalin, which composed of Histochoice-tissue frxative ; 200 m1, Sodium acetate 3 gm, Sodium hydroxide 8 gm, Sodium phosphate monobasic 10.5 gm, Sodium phosphate dibasic 19.5 gm, 40% Formaline. The rapid ftxaton in 3 % formalin in Histochoice by microwave has similar quality and sensitiviψ in H&E, histochemistry, immunohistochemistry and in situ hybridization, compared with 10% buffered formalin with or without irradiation. Total consumed time for tissue process, focus on small specimen, is 1h 37 min. Conclusion : The ftxation time was signiftcantly reduced, and rapid pathological diagnosis could be performed. The sensitivity for immunohistochemistη was qualitatively increased. The environment of gross cutting room was comfortable due to the low formalin concentration. This new method was very useful tools with simple and rapid for tissue processing and pathologic diagnosis.