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- 저자 : 장림림 ( Linlin Zhang ) (검색결과 2 건)
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잠재적인 당뇨병 생체지표로서의 일반타액단백질(CSP1)
장림림 ( Linlin Zhang ),한미희 ( Mi Hee Han ),오상욱 ( Sang Wook Oh ) 전북대학교 과학교육연구소 2018 과학과 과학교육 논문지 Vol.43 No.1
Saliva is a bodily fluid secreted by three pairs of major salivary glands and by many of minor salivary gland. The human common salivary protein 1(CSP1) is one of variety of molecules in saliva and its function remains to be determined. The saliva CSP1 levels of 17 DM patients and 9 normal adults were quantified by ELISA system. As a probe, the mAb-CSP1 was used for Western blotting of human saliva and immunohistochemistry of various human tissues. The study showed that the saliva CSP1 concentration of diabetes mellitus(DM) patients was much higher than of healthy controls. The salivary gland was the only tissue stained with mAb-CSP1 by immunohistochemistry among the various human tissues. Quantification of CSP1 concentration by ELISA showed that the median values of DM patients and healthy adults were 4.65(3.36-6.90)μg/ml and 3.74(2.86-5.02)μg/ml, respectively. Student t-test results indicated that there was a statistically significant difference between the two groups(p<0.026). Therefore, the significant difference of CSP1 levels between the two groups indicated that CSP1 is a potential biomarker for detection or screenig of DM patients.
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성병을 유발하는 N. gonorrhoeae에 대한 단일클론 항체의 생산
장림림 ( Linlin Zhang ),최안나 ( Anna Choi ),오상욱 ( Sang Wook Oh ) 전북대학교 과학교육연구소 2016 과학과 과학교육 논문지 Vol.41 No.2
Gonorrhea, one of the most common adult diseases of human society, is caused by infection with gonococcus (Neisseria gonorrhoea) through sexual contact between humans and humans, such as sexual intercourse. Gonorrhea causes inflammation of the urogenital system in both males and females, and if not treated at the right time, it can cause a large aftereffect such as infertility or blindness. Therefore, in recent years, the importance of early diagnosis that accurately diagnoses and prescribes at an appropriate time is emphasized. The purpose of this study was to produce monoclonal antibody (mAb) against to N. gonorrhoea and to use for quick diagnosis of whether people were infected with N. gonorrhoea. Spleen from immunized mouse was extracted after N. gonorrhoea was injected as an immunogen. The spleen cell was fused with myeloma SP2 cell, and then hybridoma cells producing mAb against N. gonorrhoea specifically were generated by classical method for mAb. The generated 33 mAbs will be utilized for research material as well as for diagnostic reagent for diseases.
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