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육홍선(Hong-Sun Yook),이현자(Hyun-Ja Lee),임성일(Sung-Il Im),김성(Sung Kim),변명우(Myung-Woo Byun) 한국식품영양과학회 1997 한국식품영양과학회지 Vol.26 No.6
감마선 조사선량에 따른 단백질의 변성정도를 단백질분해효소의 활성변화를 측정하여 단백질식품의 감마선 조사 허용선량기준의 기초자료를 마련하고자 하였다. 수용상태의 포유류형 serine protease와 키위 및 파인애플의 단백질분해효소활성은 5kGy 조사로 10%내의 단백질활성이 소실되었으나, 30 kGy에서는 30~65%가 소실되었다. 한편 건조상태의 pancreatin의 경우 5kGy에서 6%, 40 kGy에서 14%의 단백질분해 활성이 감소하였고, lysozyme에 있어서는 각각 10%, 20%의 항미생물활성이 감소하였다. 이러한 결과로부터 건조상태의 단백질이 수용상태보다 감마선에 대하여 더 안정하며, 5kGy내의 조사선량에서는 수용상태나 건조상태 모두 안정한 것으로 나타났다. 또한, 수용상태로 10 kGy 이상 조사된 API, α-chymotrypsin, pancreatin은 SDS-PAGE 패턴에서 단백질의 peptide 결합의 절단과 저분자화가 일어났다. Skim milk, hammastein casein, lysozyme의 in vitro 소화율과 아미노산 조성은 40 kGy 조사선량 범위내에서는 비조사군과 큰 차이를 나타내지 않았다. Effects of gamma irradiation on the activity and the properties(amino acid compositions, in vitro digestibility and SDS-PAGE pattern) of proteolytic enzymes were investigated. The proteolytic activity of soluble human serine protease, enzyme in kiwi and pineapple decreased 10% and 30~65% by 5 kGy and 30 kGy, respectively. In dried pancreatin and lysozyme, the proteolytic and antimicrobial activities decreased 6~14% and 10~20% by 5 kGy and 40 kGy, respectively. The analysis of above 10 kGy-irradiated soluble human serine protease by SDS-PAGE revealed radiolysis of the enzyme into protein or peptides of lower molecular weights. The irradiation of skim milk, hammastein casein, and lysozyme up to 40 kGy had no deleterious effect on either the in vitro digestibility or amino acid compositions.
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안정형 협심증과 급성 관상동맥 증후군 환자에서 경동맥의 재형성
박정랑 ( Jeong Rang Park ),권태정 ( Tae Jung Kwon ),강영란 ( Young Ran Kang ),박소라 ( So Ra Park ),고진신 ( Jin Sin Kho ),임성일 ( Sung Il Im ),박성지 ( Sung Ji Park ),곽충환 ( Chung Hwan Kwak ),황진용 ( Jin Yong Hwang ) 대한내과학회 2006 대한내과학회지 Vol.71 No.3
Background: Acute adaptive vascular remodeling occurs in active and unstable inflammatory plaques. It has been suggested that the adaptive coronary vascular remodeling, in patients with acute coronary syndrome (ACS), may be systemic and may show similar vascular remodeling in the carotid arteries. We investigated the ultrasonographic features of the common carotid artery (CCA) to determine whether the arterial expansive remodeling found in the coronary artery occurs in the carotid arteries of patients with ACS. Methods: We measured lumen diameter (LD), interadventitial diameter (IAD) and intima media thickness (IMT) using a B-mode ultrasound in both common carotid arteries in patients with ACS (N=74) and chronic stable angina (CSA) (N=31). Positive remodeling was arbitrarily defined as an IMTmax>1 mm and IAD>8 mm and negative remodeling as an IMTmax>1 mm and IAD<7 mm. Other values were defined as no remodeling. Results: There were no significant differences in LD IAD and maximal IMT of the right CCA and the left CCA in comparisons between the ACS and the CSA patient groups. There were no differences for number of cases with no remodeling or differences in positive and negative remodeling in the right common carotid artery and left common carotid artery in comparisons between the ACS and CSA patient groups. . Presence of plaque in both common carotid arteries showed similar frequency in the ACS and CSA patient groups. The characteristics of carotid artery plaques were not different in the two groups. The remodeling index (IAD/LD) was correlated with IMTmax (right CCA r=0.797, p<0.001; left CCA r=0.860, p<0.001). Conclusions: The common carotid arterial structure of ACS patients was not different from that of CSA patients. Therefore, these results suggest that the expansive arterial remodeling, due to coronary inflammatory plaques, appears to take place locally rather than systemically.(Korean J Med 71:266-275, 2006)
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