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Nested-PCR법과 Competitive PCR법을 이용한 뽕나무 오갈병(MD) Phytoplasma의 검출과 밀도변화
채승민 ( Seung Min Chae ),이솔 ( Sol Lee ),차병진 ( Byeong Jin Cha ),이혁인 ( Hyok In Lee ),한상섭 ( Sang Sub Han ) 한국산림과학회 2011 한국산림과학회지 Vol.100 No.4
The detectable levels and population fluctuations of phytoplasmas infecting dwarf mulberry trees were investigated using nested-PCR and competitive-PCR methods. Samples of five different types were studied : A. petiole of a leaf that displays dwarf symptoms, B. petiole from apparently healthy leaf residing on a branch also supports a leaf with dwarf symptoms, C. the branch portion that supports a leaf with dwarf symptoms, D. the leaf petiole from healthy appearing leaves on branch with no dwarf symptoms, and branch portion of branch with no dwarf symptoms, E. the rootlets of trees with dwarf symptoms. These 5-parts were collected from each tree during June - April, once in every two months. The phytoplasma was detected from all parts of collected mulberry samples during all seasons using nested-PCR with AS-1/AS-2 primer pairs. The phytoplasma was detected until 104 dilution using direct-PCR method, but it was detected until 1013 dilution by the nested-PCR method. The density of pytoplasma was found to be 7.94×1018-1012copies/μL in mulberry trees. The density of phytoplasma was observed throughout the year in all samples of mulberry trees. The highest rates of phytoplasma was found in the samples B and C during the early growing season followed by the sample A and D during the dormant season. Samples C and E displayed the highest phytoplasma density followed sample D. The density of phytoplasma appeared stable during all the seasons for samples C and A. The result of the present study demonstrates the utility of nested-PCR and competitive-PCR for detection and determination of population fluctuations of phytoplasmas in plant tissues.
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Pseudomonas syringae pv. syringae에 의한 수박 잎점무늬병의 발생
박경수(Kyoung-Soo Park),이지혜(Ji-Hye Lee),김영탁(Young-Tak Kim),김혜성(Hye-Seong Kim),이준우(June-woo Lee),이현수(Hyun-Su Lee),이혁인(Hyok-In Lee),차재순(Jae-Soon Cha) 한국식물병리학회 2021 식물병연구 Vol.27 No.4
Typical bacterial symptoms, water-soaking brown and black leaf spots with yellow halo, were observed on watermelon seedlings in nursery and field of Gyeongnam and Jeonnam provinces. Bacterial isolates from the lesion showed strong pathogenicity on watermelon and zucchini. One of them was rod-shaped with 4 polar flagella by observation of transmission electron microscopy. They belonged to LOPAT group 1. The phylogen- ical trees with nucleotide sequences of 16S rRNA and multi-locus sequencing typing with the 4 house-keep- ing genes (gapA, gltA, gyrB, and rpoD) of the isolates showed they were highly homologous to Pseudomonas syringae pv. syringae and grouped together with them, indicating that they were appeared as P. syringae genomospecies group 1. Morphological, physiological, and genetical characteristics of the isolates suggested they are P. syringae pv. syringae. We believe this is the first report that P. syringae pv. syringae caused leaf spot disease on watermelon in the Republic of Korea.
ScienceON
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