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전혜진 ( Hye Jin Jeon ),이초롱 ( Chorong Lee ),김범근 ( Bum Keun Kim ),김수미 ( Sumi Kim ),장광일 ( Gwang Il Jang ),이강윤 ( Gahngyoon Rhee ),권혜민 ( Hyemin Kwon ),한지은 ( Jee Eun Han ) 한국수산과학회 2021 한국수산과학회지 Vol.54 No.5
Vibrio parahaemolyticus associated with acute hepatopancreatic necrosis disease (Vp<sub>AHPND</sub>) and Enterocytozoon hepatopenaei (EHP) are the two most important pathogens in shrimp aquaculture and they have caused enormous losses to the shrimp industry worldwide. In ponds, the major target organ for the two pathogens is the hepatopancreas, and infection with EHP is a known potential risk factor for Vp<sub>AHPND</sub> infection. This study aimed to develop a PCR (polymerase chain reaction)-based diagnostic method for simultaneously detecting Vp<sub>AHPND</sub> and EHP. The newly developed PCR diagnostic method could be used to test various samples, such as seawater, shrimp, and feces. The diagnostic method exhibited high sensitivity and specificity for both pathogens. This will help reduce the potential economic losses that may have been caused by the two major shrimp pathogens, Vp<sub>AHPND</sub> and EHP, and will allow for the efforts and time spent combatting them to be dedicated elsewhere.